A solid-phase affinity labeling method for target-selective isolation and modification of proteins

Daichi Kuwahara, Takahiro Hasumi, Hajime Kaneko, Madoka Unno, Daisuke Takahashi, Kazunobu Toshima

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Solid-phase affinity labeling of a target protein, peanut agglutinin (PNA), with the specifically designed chemical tool 1 selectively and effectively furnished the labeled PNA. Furthermore, this method was applicable to native human carbonic anhydrase II in red blood cell lysate using the chemical tool 2 without the need for tedious manipulations. This journal is

Original languageEnglish
Pages (from-to)15601-15604
Number of pages4
JournalChemical Communications
Volume50
Issue number98
DOIs
Publication statusPublished - 2014 Dec 21

Fingerprint

Peanut Agglutinin
Labeling
Carbonic anhydrase
Carbonic Anhydrase II
Proteins
Blood
Cells

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Ceramics and Composites
  • Electronic, Optical and Magnetic Materials
  • Surfaces, Coatings and Films
  • Materials Chemistry
  • Metals and Alloys

Cite this

A solid-phase affinity labeling method for target-selective isolation and modification of proteins. / Kuwahara, Daichi; Hasumi, Takahiro; Kaneko, Hajime; Unno, Madoka; Takahashi, Daisuke; Toshima, Kazunobu.

In: Chemical Communications, Vol. 50, No. 98, 21.12.2014, p. 15601-15604.

Research output: Contribution to journalArticle

Kuwahara, Daichi ; Hasumi, Takahiro ; Kaneko, Hajime ; Unno, Madoka ; Takahashi, Daisuke ; Toshima, Kazunobu. / A solid-phase affinity labeling method for target-selective isolation and modification of proteins. In: Chemical Communications. 2014 ; Vol. 50, No. 98. pp. 15601-15604.
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