Abstract
Few studies have been conducted to investigate biological factors that affect postoperative knee motion after total knee arthroplasty (TKA). The purpose of this study is to test the hypothesis that range of knee motion (ROM) at 4 weeks after TKA is correlated with the concentration of extracellular matrix metalloproteinase inducer (EMMPRIN) and transforming growth factor (TGF)-beta1 in the exudative fluid harvested from the joint after surgery. A prospective measurement study was conducted with 20 osteoarthritis patients who underwent TKA. At 48 h after surgery, the exudate was harvested from a closed drainage system. Enzyme-linked immunosorbent assay was performed to measure the concentration of TGF-beta1, EMMPRIN, MMP-1, 2, 9, tissue inhibitor of metalloproteinase-1, and Hyalunonan. Knee flexion angle was measured before and at 4 weeks after surgery. There was a significant correlation between the EMMPRIN levels and knee flexion angle (r = 0.557, p = 0.0148). Western blot analysis of the exudate showed a prominent band for EMMPRIN at 27 kDa. On the other hand, there was no correlation between the TGF-beta1 levels and the knee flexion angle. This study showed that EMMPRIN levels after TKA affect the postoperative ROM. As to clinical relevance, EMMPRIN in the exudate after TKA is a promising biological indicator to predict difficulty in restoring postoperative ROM.
| Original language | English |
|---|---|
| Pages (from-to) | 298-304 |
| Number of pages | 7 |
| Journal | Knee Surgery, Sports Traumatology, Arthroscopy |
| Volume | 17 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2009 Mar |
| Externally published | Yes |
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Keywords
- EMMPRIN
- Fibrosis
- MMP
- Range of knee motion
- Total knee arthroplasty
- Transforming growth factor
ASJC Scopus subject areas
- Orthopedics and Sports Medicine
- Surgery
Cite this
A soluble factor (EMMPRIN) in exudate influences knee motion after total arthroplasty. / Onodera, Jun; Onodera, Shin; Kondo, Eiji; Betsuyaku, Tomoko; Yasuda, Kazunori.
In: Knee Surgery, Sports Traumatology, Arthroscopy, Vol. 17, No. 3, 03.2009, p. 298-304.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A soluble factor (EMMPRIN) in exudate influences knee motion after total arthroplasty
AU - Onodera, Jun
AU - Onodera, Shin
AU - Kondo, Eiji
AU - Betsuyaku, Tomoko
AU - Yasuda, Kazunori
PY - 2009/3
Y1 - 2009/3
N2 - Few studies have been conducted to investigate biological factors that affect postoperative knee motion after total knee arthroplasty (TKA). The purpose of this study is to test the hypothesis that range of knee motion (ROM) at 4 weeks after TKA is correlated with the concentration of extracellular matrix metalloproteinase inducer (EMMPRIN) and transforming growth factor (TGF)-beta1 in the exudative fluid harvested from the joint after surgery. A prospective measurement study was conducted with 20 osteoarthritis patients who underwent TKA. At 48 h after surgery, the exudate was harvested from a closed drainage system. Enzyme-linked immunosorbent assay was performed to measure the concentration of TGF-beta1, EMMPRIN, MMP-1, 2, 9, tissue inhibitor of metalloproteinase-1, and Hyalunonan. Knee flexion angle was measured before and at 4 weeks after surgery. There was a significant correlation between the EMMPRIN levels and knee flexion angle (r = 0.557, p = 0.0148). Western blot analysis of the exudate showed a prominent band for EMMPRIN at 27 kDa. On the other hand, there was no correlation between the TGF-beta1 levels and the knee flexion angle. This study showed that EMMPRIN levels after TKA affect the postoperative ROM. As to clinical relevance, EMMPRIN in the exudate after TKA is a promising biological indicator to predict difficulty in restoring postoperative ROM.
AB - Few studies have been conducted to investigate biological factors that affect postoperative knee motion after total knee arthroplasty (TKA). The purpose of this study is to test the hypothesis that range of knee motion (ROM) at 4 weeks after TKA is correlated with the concentration of extracellular matrix metalloproteinase inducer (EMMPRIN) and transforming growth factor (TGF)-beta1 in the exudative fluid harvested from the joint after surgery. A prospective measurement study was conducted with 20 osteoarthritis patients who underwent TKA. At 48 h after surgery, the exudate was harvested from a closed drainage system. Enzyme-linked immunosorbent assay was performed to measure the concentration of TGF-beta1, EMMPRIN, MMP-1, 2, 9, tissue inhibitor of metalloproteinase-1, and Hyalunonan. Knee flexion angle was measured before and at 4 weeks after surgery. There was a significant correlation between the EMMPRIN levels and knee flexion angle (r = 0.557, p = 0.0148). Western blot analysis of the exudate showed a prominent band for EMMPRIN at 27 kDa. On the other hand, there was no correlation between the TGF-beta1 levels and the knee flexion angle. This study showed that EMMPRIN levels after TKA affect the postoperative ROM. As to clinical relevance, EMMPRIN in the exudate after TKA is a promising biological indicator to predict difficulty in restoring postoperative ROM.
KW - EMMPRIN
KW - Fibrosis
KW - MMP
KW - Range of knee motion
KW - Total knee arthroplasty
KW - Transforming growth factor
UR - http://www.scopus.com/inward/record.url?scp=65049089498&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=65049089498&partnerID=8YFLogxK
U2 - 10.1007/s00167-008-0688-6
DO - 10.1007/s00167-008-0688-6
M3 - Article
VL - 17
SP - 298
EP - 304
JO - Knee Surgery, Sports Traumatology, Arthroscopy
JF - Knee Surgery, Sports Traumatology, Arthroscopy
SN - 0942-2056
IS - 3
ER -