A Statistical Method of Identifying Interactions in Neuron–Glia Systems Based on Functional Multicell Ca2+ Imaging

Ken Nakae, Yuji Ikegaya, Tomoe Ishikawa, Shigeyuki Oba, Hidetoshi Urakubo, Masanori Koyama, Shin Ishii

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Crosstalk between neurons and glia may constitute a significant part of information processing in the brain. We present a novel method of statistically identifying interactions in a neuron–glia network. We attempted to identify neuron–glia interactions from neuronal and glial activities via maximum-a-posteriori (MAP)-based parameter estimation by developing a generalized linear model (GLM) of a neuron–glia network. The interactions in our interest included functional connectivity and response functions. We evaluated the cross-validated likelihood of GLMs that resulted from the addition or removal of connections to confirm the existence of specific neuron-to-glia or glia-to-neuron connections. We only accepted addition or removal when the modification improved the cross-validated likelihood. We applied the method to a high-throughput, multicellular in vitro Ca2+ imaging dataset obtained from the CA3 region of a rat hippocampus, and then evaluated the reliability of connectivity estimates using a statistical test based on a surrogate method. Our findings based on the estimated connectivity were in good agreement with currently available physiological knowledge, suggesting our method can elucidate undiscovered functions of neuron–glia systems.

Original languageEnglish
JournalPLoS Computational Biology
Volume10
Issue number11
DOIs
Publication statusPublished - 2014 Nov 1
Externally publishedYes

Fingerprint

Neuroglia
Statistical method
connectivity
Statistical methods
statistical analysis
Imaging
image analysis
Neurons
Imaging techniques
calcium
Neuron
Connectivity
neurons
surrogate method
Interaction
Likelihood
information processing
Hippocampus
brain
Maximum a Posteriori

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Modelling and Simulation
  • Ecology
  • Molecular Biology
  • Genetics
  • Cellular and Molecular Neuroscience
  • Computational Theory and Mathematics

Cite this

A Statistical Method of Identifying Interactions in Neuron–Glia Systems Based on Functional Multicell Ca2+ Imaging. / Nakae, Ken; Ikegaya, Yuji; Ishikawa, Tomoe; Oba, Shigeyuki; Urakubo, Hidetoshi; Koyama, Masanori; Ishii, Shin.

In: PLoS Computational Biology, Vol. 10, No. 11, 01.11.2014.

Research output: Contribution to journalArticle

Nakae, Ken ; Ikegaya, Yuji ; Ishikawa, Tomoe ; Oba, Shigeyuki ; Urakubo, Hidetoshi ; Koyama, Masanori ; Ishii, Shin. / A Statistical Method of Identifying Interactions in Neuron–Glia Systems Based on Functional Multicell Ca2+ Imaging. In: PLoS Computational Biology. 2014 ; Vol. 10, No. 11.
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