A stimulatory effect of recombinant murine interleukin-7 (IL-7) on B-cell colony formation and an inhibitory effect of IL-1α

T. Suda, S. Okada, J. Suda, Y. Miura, M. Ito, T. Sudo, S. I. Hayashi, S. I. Nishikawa, H. Nakauchi

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Abstract

Using a clonal culture system, we investigated the lymphohematopoietic effects of recombinant interleukin-7 (IL-7) obtained from conditioned media of transfected COS 1 cells. IL-7 alone acted on murine bone marrow cells and supported the formation of B-cell colonies. These colony cells were positive for B220, and some of them were also found to have either IgM or Thy-1. B220+, IgM- cells, but not B220- cells sorted from fresh bone marrow cells were able to form B cell colonies in the presence of IL-7. Thus, IL-7 supported the differentiation of B220+, IgM- cells to B220+, IgM+ cells. B220+, IgM+ cells did not proliferate in the presence of IL-7. IL-7 did not affect the myeloid colony formation supported by IL-3, IL-5, IL-6, granulocyte macrophage colony stimulating factor (GM-CSF), and G-CSF. On the other hand, lymphocyte colony formation was not affected by IL-2, IL-3, IL-4, IL-5, IL-6, GM-CSF, or G-CSF. Interestingly, IL-1α inhibited IL-7-induced B cell colony formation in a dose-dependent manner, while the same concentration of IL-1α enhanced the myeloid colony formation by IL-3. This reciprocal effect of IL-1α may act on hematopoietic progenitor cells without accessory cells. These data show that IL-7 is a B cell growth factor and that IL-1α may play an important role in differentiation of myeloid and lymphoid lineages.

Original languageEnglish
Pages (from-to)1936-1941
Number of pages6
JournalBlood
Volume74
Issue number6
Publication statusPublished - 1989

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Interleukin-7
Interleukin-1
B-Lymphocytes
Cells
Immunoglobulin M
Interleukin-3
Interleukin-5
Granulocyte Colony-Stimulating Factor
Granulocyte-Macrophage Colony-Stimulating Factor
Bone Marrow Cells
Interleukin-4
Interleukin-6
Bone
Lymphocytes
COS Cells
Accessories
Cell growth
Conditioned Culture Medium
Hematopoietic Stem Cells
Interleukin-2

ASJC Scopus subject areas

  • Hematology

Cite this

Suda, T., Okada, S., Suda, J., Miura, Y., Ito, M., Sudo, T., ... Nakauchi, H. (1989). A stimulatory effect of recombinant murine interleukin-7 (IL-7) on B-cell colony formation and an inhibitory effect of IL-1α. Blood, 74(6), 1936-1941.

A stimulatory effect of recombinant murine interleukin-7 (IL-7) on B-cell colony formation and an inhibitory effect of IL-1α. / Suda, T.; Okada, S.; Suda, J.; Miura, Y.; Ito, M.; Sudo, T.; Hayashi, S. I.; Nishikawa, S. I.; Nakauchi, H.

In: Blood, Vol. 74, No. 6, 1989, p. 1936-1941.

Research output: Contribution to journalArticle

Suda, T, Okada, S, Suda, J, Miura, Y, Ito, M, Sudo, T, Hayashi, SI, Nishikawa, SI & Nakauchi, H 1989, 'A stimulatory effect of recombinant murine interleukin-7 (IL-7) on B-cell colony formation and an inhibitory effect of IL-1α', Blood, vol. 74, no. 6, pp. 1936-1941.
Suda, T. ; Okada, S. ; Suda, J. ; Miura, Y. ; Ito, M. ; Sudo, T. ; Hayashi, S. I. ; Nishikawa, S. I. ; Nakauchi, H. / A stimulatory effect of recombinant murine interleukin-7 (IL-7) on B-cell colony formation and an inhibitory effect of IL-1α. In: Blood. 1989 ; Vol. 74, No. 6. pp. 1936-1941.
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abstract = "Using a clonal culture system, we investigated the lymphohematopoietic effects of recombinant interleukin-7 (IL-7) obtained from conditioned media of transfected COS 1 cells. IL-7 alone acted on murine bone marrow cells and supported the formation of B-cell colonies. These colony cells were positive for B220, and some of them were also found to have either IgM or Thy-1. B220+, IgM- cells, but not B220- cells sorted from fresh bone marrow cells were able to form B cell colonies in the presence of IL-7. Thus, IL-7 supported the differentiation of B220+, IgM- cells to B220+, IgM+ cells. B220+, IgM+ cells did not proliferate in the presence of IL-7. IL-7 did not affect the myeloid colony formation supported by IL-3, IL-5, IL-6, granulocyte macrophage colony stimulating factor (GM-CSF), and G-CSF. On the other hand, lymphocyte colony formation was not affected by IL-2, IL-3, IL-4, IL-5, IL-6, GM-CSF, or G-CSF. Interestingly, IL-1α inhibited IL-7-induced B cell colony formation in a dose-dependent manner, while the same concentration of IL-1α enhanced the myeloid colony formation by IL-3. This reciprocal effect of IL-1α may act on hematopoietic progenitor cells without accessory cells. These data show that IL-7 is a B cell growth factor and that IL-1α may play an important role in differentiation of myeloid and lymphoid lineages.",
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