A subtype of opioid κ-receptor is coupled to inhibition of Gil-mediated phospholipase C activity in the guinea pig cerebellum

Hidemi Misawa, Hiroshi Ueda, Toshiaki Katada, Michio Ui, Masamichi Satoh

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

PLC activity was stimulated either by 1-100 μM of GTP or by 100-3,000 μM Ca2+ in lysed synaptosomal membranes of the guinea pig cerebellum. The κ-opioid receptor agonist selectively inhibited the PLC activity stimulated by 100 μM GTP, but not by 100-3,000 μM Ca2+. Pretreatment of membranes with PTX abolished such a κ-agonist-induced inhibition of PLC activity. The reconstitution of Gi1, but not of Go purified from porcine brains with PTX-treated membranes showed a complete recovery of the κ-agonist-inhibition of PLC activity. These findings suggest that a novel subtype κ-receptor mediates inhibition of PLC through inhibiting the intrinsic activity of PTX-substrate G-proteins.

Original languageEnglish
Pages (from-to)106-110
Number of pages5
JournalFEBS Letters
Volume361
Issue number1
DOIs
Publication statusPublished - 1995 Mar 13
Externally publishedYes

Fingerprint

Opioid Receptors
Type C Phospholipases
Programmable logic controllers
Cerebellum
Guinea Pigs
Guanosine Triphosphate
Membranes
GTP-Binding Proteins
Swine
Brain
Recovery
Substrates

Keywords

  • Gi1-type G-protein
  • Opioid κ-receptor
  • Phospholipase C

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

A subtype of opioid κ-receptor is coupled to inhibition of Gil-mediated phospholipase C activity in the guinea pig cerebellum. / Misawa, Hidemi; Ueda, Hiroshi; Katada, Toshiaki; Ui, Michio; Satoh, Masamichi.

In: FEBS Letters, Vol. 361, No. 1, 13.03.1995, p. 106-110.

Research output: Contribution to journalArticle

Misawa, Hidemi ; Ueda, Hiroshi ; Katada, Toshiaki ; Ui, Michio ; Satoh, Masamichi. / A subtype of opioid κ-receptor is coupled to inhibition of Gil-mediated phospholipase C activity in the guinea pig cerebellum. In: FEBS Letters. 1995 ; Vol. 361, No. 1. pp. 106-110.
@article{300e3e96ea954ea391e8c350808340cf,
title = "A subtype of opioid κ-receptor is coupled to inhibition of Gil-mediated phospholipase C activity in the guinea pig cerebellum",
abstract = "PLC activity was stimulated either by 1-100 μM of GTP or by 100-3,000 μM Ca2+ in lysed synaptosomal membranes of the guinea pig cerebellum. The κ-opioid receptor agonist selectively inhibited the PLC activity stimulated by 100 μM GTP, but not by 100-3,000 μM Ca2+. Pretreatment of membranes with PTX abolished such a κ-agonist-induced inhibition of PLC activity. The reconstitution of Gi1, but not of Go purified from porcine brains with PTX-treated membranes showed a complete recovery of the κ-agonist-inhibition of PLC activity. These findings suggest that a novel subtype κ-receptor mediates inhibition of PLC through inhibiting the intrinsic activity of PTX-substrate G-proteins.",
keywords = "Gi1-type G-protein, Opioid κ-receptor, Phospholipase C",
author = "Hidemi Misawa and Hiroshi Ueda and Toshiaki Katada and Michio Ui and Masamichi Satoh",
year = "1995",
month = "3",
day = "13",
doi = "10.1016/0014-5793(95)00162-3",
language = "English",
volume = "361",
pages = "106--110",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - A subtype of opioid κ-receptor is coupled to inhibition of Gil-mediated phospholipase C activity in the guinea pig cerebellum

AU - Misawa, Hidemi

AU - Ueda, Hiroshi

AU - Katada, Toshiaki

AU - Ui, Michio

AU - Satoh, Masamichi

PY - 1995/3/13

Y1 - 1995/3/13

N2 - PLC activity was stimulated either by 1-100 μM of GTP or by 100-3,000 μM Ca2+ in lysed synaptosomal membranes of the guinea pig cerebellum. The κ-opioid receptor agonist selectively inhibited the PLC activity stimulated by 100 μM GTP, but not by 100-3,000 μM Ca2+. Pretreatment of membranes with PTX abolished such a κ-agonist-induced inhibition of PLC activity. The reconstitution of Gi1, but not of Go purified from porcine brains with PTX-treated membranes showed a complete recovery of the κ-agonist-inhibition of PLC activity. These findings suggest that a novel subtype κ-receptor mediates inhibition of PLC through inhibiting the intrinsic activity of PTX-substrate G-proteins.

AB - PLC activity was stimulated either by 1-100 μM of GTP or by 100-3,000 μM Ca2+ in lysed synaptosomal membranes of the guinea pig cerebellum. The κ-opioid receptor agonist selectively inhibited the PLC activity stimulated by 100 μM GTP, but not by 100-3,000 μM Ca2+. Pretreatment of membranes with PTX abolished such a κ-agonist-induced inhibition of PLC activity. The reconstitution of Gi1, but not of Go purified from porcine brains with PTX-treated membranes showed a complete recovery of the κ-agonist-inhibition of PLC activity. These findings suggest that a novel subtype κ-receptor mediates inhibition of PLC through inhibiting the intrinsic activity of PTX-substrate G-proteins.

KW - Gi1-type G-protein

KW - Opioid κ-receptor

KW - Phospholipase C

UR - http://www.scopus.com/inward/record.url?scp=0028987260&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028987260&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(95)00162-3

DO - 10.1016/0014-5793(95)00162-3

M3 - Article

C2 - 7890026

AN - SCOPUS:0028987260

VL - 361

SP - 106

EP - 110

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 1

ER -