Abstract
PLC activity was stimulated either by 1-100 μM of GTP or by 100-3,000 μM Ca2+ in lysed synaptosomal membranes of the guinea pig cerebellum. The κ-opioid receptor agonist selectively inhibited the PLC activity stimulated by 100 μM GTP, but not by 100-3,000 μM Ca2+. Pretreatment of membranes with PTX abolished such a κ-agonist-induced inhibition of PLC activity. The reconstitution of Gi1, but not of Go purified from porcine brains with PTX-treated membranes showed a complete recovery of the κ-agonist-inhibition of PLC activity. These findings suggest that a novel subtype κ-receptor mediates inhibition of PLC through inhibiting the intrinsic activity of PTX-substrate G-proteins.
Original language | English |
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Pages (from-to) | 106-110 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 361 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1995 Mar 13 |
Externally published | Yes |
Keywords
- Gi1-type G-protein
- Opioid κ-receptor
- Phospholipase C
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology