A subtype of opioid κ-receptor is coupled to inhibition of Gil-mediated phospholipase C activity in the guinea pig cerebellum

Hidemi Misawa, Hiroshi Ueda, Toshiaki Katada, Michio Ui, Masamichi Satoh

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

PLC activity was stimulated either by 1-100 μM of GTP or by 100-3,000 μM Ca2+ in lysed synaptosomal membranes of the guinea pig cerebellum. The κ-opioid receptor agonist selectively inhibited the PLC activity stimulated by 100 μM GTP, but not by 100-3,000 μM Ca2+. Pretreatment of membranes with PTX abolished such a κ-agonist-induced inhibition of PLC activity. The reconstitution of Gi1, but not of Go purified from porcine brains with PTX-treated membranes showed a complete recovery of the κ-agonist-inhibition of PLC activity. These findings suggest that a novel subtype κ-receptor mediates inhibition of PLC through inhibiting the intrinsic activity of PTX-substrate G-proteins.

Original languageEnglish
Pages (from-to)106-110
Number of pages5
JournalFEBS Letters
Volume361
Issue number1
DOIs
Publication statusPublished - 1995 Mar 13
Externally publishedYes

Keywords

  • Gi1-type G-protein
  • Opioid κ-receptor
  • Phospholipase C

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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