A subtype of opioid κ-receptor is coupled to inhibition of Gil-mediated phospholipase C activity in the guinea pig cerebellum

Hidemi Misawa; Hiroshi Ueda; Toshiaki Katada; Michio Ui; Masamichi Satoh

doi:10.1016/0014-5793(95)00162-3

A subtype of opioid κ-receptor is coupled to inhibition of Gil-mediated phospholipase C activity in the guinea pig cerebellum

Hidemi Misawa, Hiroshi Ueda, Toshiaki Katada, Michio Ui, Masamichi Satoh

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

PLC activity was stimulated either by 1-100 μM of GTP or by 100-3,000 μM Ca²⁺ in lysed synaptosomal membranes of the guinea pig cerebellum. The κ-opioid receptor agonist selectively inhibited the PLC activity stimulated by 100 μM GTP, but not by 100-3,000 μM Ca²⁺. Pretreatment of membranes with PTX abolished such a κ-agonist-induced inhibition of PLC activity. The reconstitution of Gi1, but not of Go purified from porcine brains with PTX-treated membranes showed a complete recovery of the κ-agonist-inhibition of PLC activity. These findings suggest that a novel subtype κ-receptor mediates inhibition of PLC through inhibiting the intrinsic activity of PTX-substrate G-proteins.

Original language	English
Pages (from-to)	106-110
Number of pages	5
Journal	FEBS Letters
Volume	361
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(95)00162-3
Publication status	Published - 1995 Mar 13
Externally published	Yes

Keywords

Gi1-type G-protein
Opioid κ-receptor
Phospholipase C

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(95)00162-3

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title = "A subtype of opioid κ-receptor is coupled to inhibition of Gil-mediated phospholipase C activity in the guinea pig cerebellum",

abstract = "PLC activity was stimulated either by 1-100 μM of GTP or by 100-3,000 μM Ca2+ in lysed synaptosomal membranes of the guinea pig cerebellum. The κ-opioid receptor agonist selectively inhibited the PLC activity stimulated by 100 μM GTP, but not by 100-3,000 μM Ca2+. Pretreatment of membranes with PTX abolished such a κ-agonist-induced inhibition of PLC activity. The reconstitution of Gi1, but not of Go purified from porcine brains with PTX-treated membranes showed a complete recovery of the κ-agonist-inhibition of PLC activity. These findings suggest that a novel subtype κ-receptor mediates inhibition of PLC through inhibiting the intrinsic activity of PTX-substrate G-proteins.",

keywords = "Gi1-type G-protein, Opioid κ-receptor, Phospholipase C",

author = "Hidemi Misawa and Hiroshi Ueda and Toshiaki Katada and Michio Ui and Masamichi Satoh",

note = "Funding Information: Acknowledgments.{"} This work has been done at the Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kyoto University. Parts of this study were supported by research grants from the Ministry of Education, Science and Culture, Japan and from Uehara Memorial Foundation, Kowa Life Science Foundation, Terumo Life Science Foundation Research Foundation for Pharmaceutical Sciences and Kato Memorial Foundation.",

year = "1995",

month = mar,

day = "13",

doi = "10.1016/0014-5793(95)00162-3",

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T1 - A subtype of opioid κ-receptor is coupled to inhibition of Gil-mediated phospholipase C activity in the guinea pig cerebellum

AU - Misawa, Hidemi

AU - Ueda, Hiroshi

AU - Katada, Toshiaki

AU - Ui, Michio

AU - Satoh, Masamichi

N1 - Funding Information: Acknowledgments." This work has been done at the Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kyoto University. Parts of this study were supported by research grants from the Ministry of Education, Science and Culture, Japan and from Uehara Memorial Foundation, Kowa Life Science Foundation, Terumo Life Science Foundation Research Foundation for Pharmaceutical Sciences and Kato Memorial Foundation.

PY - 1995/3/13

Y1 - 1995/3/13

N2 - PLC activity was stimulated either by 1-100 μM of GTP or by 100-3,000 μM Ca2+ in lysed synaptosomal membranes of the guinea pig cerebellum. The κ-opioid receptor agonist selectively inhibited the PLC activity stimulated by 100 μM GTP, but not by 100-3,000 μM Ca2+. Pretreatment of membranes with PTX abolished such a κ-agonist-induced inhibition of PLC activity. The reconstitution of Gi1, but not of Go purified from porcine brains with PTX-treated membranes showed a complete recovery of the κ-agonist-inhibition of PLC activity. These findings suggest that a novel subtype κ-receptor mediates inhibition of PLC through inhibiting the intrinsic activity of PTX-substrate G-proteins.

AB - PLC activity was stimulated either by 1-100 μM of GTP or by 100-3,000 μM Ca2+ in lysed synaptosomal membranes of the guinea pig cerebellum. The κ-opioid receptor agonist selectively inhibited the PLC activity stimulated by 100 μM GTP, but not by 100-3,000 μM Ca2+. Pretreatment of membranes with PTX abolished such a κ-agonist-induced inhibition of PLC activity. The reconstitution of Gi1, but not of Go purified from porcine brains with PTX-treated membranes showed a complete recovery of the κ-agonist-inhibition of PLC activity. These findings suggest that a novel subtype κ-receptor mediates inhibition of PLC through inhibiting the intrinsic activity of PTX-substrate G-proteins.

KW - Gi1-type G-protein

KW - Opioid κ-receptor

KW - Phospholipase C

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A subtype of opioid κ-receptor is coupled to inhibition of Gil-mediated phospholipase C activity in the guinea pig cerebellum

Abstract

Keywords

ASJC Scopus subject areas

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