Abstract
PLC activity was stimulated either by 1-100 μM of GTP or by 100-3,000 μM Ca2+ in lysed synaptosomal membranes of the guinea pig cerebellum. The κ-opioid receptor agonist selectively inhibited the PLC activity stimulated by 100 μM GTP, but not by 100-3,000 μM Ca2+. Pretreatment of membranes with PTX abolished such a κ-agonist-induced inhibition of PLC activity. The reconstitution of Gi1, but not of Go purified from porcine brains with PTX-treated membranes showed a complete recovery of the κ-agonist-inhibition of PLC activity. These findings suggest that a novel subtype κ-receptor mediates inhibition of PLC through inhibiting the intrinsic activity of PTX-substrate G-proteins.
| Original language | English |
|---|---|
| Pages (from-to) | 106-110 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 361 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1995 Mar 13 |
| Externally published | Yes |
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Keywords
- Gi1-type G-protein
- Opioid κ-receptor
- Phospholipase C
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Cell Biology
- Genetics
- Molecular Biology
- Structural Biology
Cite this
A subtype of opioid κ-receptor is coupled to inhibition of Gil-mediated phospholipase C activity in the guinea pig cerebellum. / Misawa, Hidemi; Ueda, Hiroshi; Katada, Toshiaki; Ui, Michio; Satoh, Masamichi.
In: FEBS Letters, Vol. 361, No. 1, 13.03.1995, p. 106-110.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A subtype of opioid κ-receptor is coupled to inhibition of Gil-mediated phospholipase C activity in the guinea pig cerebellum
AU - Misawa, Hidemi
AU - Ueda, Hiroshi
AU - Katada, Toshiaki
AU - Ui, Michio
AU - Satoh, Masamichi
PY - 1995/3/13
Y1 - 1995/3/13
N2 - PLC activity was stimulated either by 1-100 μM of GTP or by 100-3,000 μM Ca2+ in lysed synaptosomal membranes of the guinea pig cerebellum. The κ-opioid receptor agonist selectively inhibited the PLC activity stimulated by 100 μM GTP, but not by 100-3,000 μM Ca2+. Pretreatment of membranes with PTX abolished such a κ-agonist-induced inhibition of PLC activity. The reconstitution of Gi1, but not of Go purified from porcine brains with PTX-treated membranes showed a complete recovery of the κ-agonist-inhibition of PLC activity. These findings suggest that a novel subtype κ-receptor mediates inhibition of PLC through inhibiting the intrinsic activity of PTX-substrate G-proteins.
AB - PLC activity was stimulated either by 1-100 μM of GTP or by 100-3,000 μM Ca2+ in lysed synaptosomal membranes of the guinea pig cerebellum. The κ-opioid receptor agonist selectively inhibited the PLC activity stimulated by 100 μM GTP, but not by 100-3,000 μM Ca2+. Pretreatment of membranes with PTX abolished such a κ-agonist-induced inhibition of PLC activity. The reconstitution of Gi1, but not of Go purified from porcine brains with PTX-treated membranes showed a complete recovery of the κ-agonist-inhibition of PLC activity. These findings suggest that a novel subtype κ-receptor mediates inhibition of PLC through inhibiting the intrinsic activity of PTX-substrate G-proteins.
KW - Gi1-type G-protein
KW - Opioid κ-receptor
KW - Phospholipase C
UR - http://www.scopus.com/inward/record.url?scp=0028987260&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028987260&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(95)00162-3
DO - 10.1016/0014-5793(95)00162-3
M3 - Article
C2 - 7890026
AN - SCOPUS:0028987260
VL - 361
SP - 106
EP - 110
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1
ER -