A subtype of opioid κ-receptor is coupled to inhibition of Gil-mediated phospholipase C activity in the guinea pig cerebellum

Hidemi Misawa; Hiroshi Ueda; Toshiaki Katada; Michio Ui; Masamichi Satoh

doi:10.1016/0014-5793(95)00162-3

A subtype of opioid κ-receptor is coupled to inhibition of Gil-mediated phospholipase C activity in the guinea pig cerebellum

Hidemi Misawa, Hiroshi Ueda, Toshiaki Katada, Michio Ui, Masamichi Satoh

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

PLC activity was stimulated either by 1-100 μM of GTP or by 100-3,000 μM Ca²⁺ in lysed synaptosomal membranes of the guinea pig cerebellum. The κ-opioid receptor agonist selectively inhibited the PLC activity stimulated by 100 μM GTP, but not by 100-3,000 μM Ca²⁺. Pretreatment of membranes with PTX abolished such a κ-agonist-induced inhibition of PLC activity. The reconstitution of Gi1, but not of Go purified from porcine brains with PTX-treated membranes showed a complete recovery of the κ-agonist-inhibition of PLC activity. These findings suggest that a novel subtype κ-receptor mediates inhibition of PLC through inhibiting the intrinsic activity of PTX-substrate G-proteins.

Original language	English
Pages (from-to)	106-110
Number of pages	5
Journal	FEBS Letters
Volume	361
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(95)00162-3
Publication status	Published - 1995 Mar 13
Externally published	Yes

Keywords

Gi1-type G-protein
Opioid κ-receptor
Phospholipase C

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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title = "A subtype of opioid κ-receptor is coupled to inhibition of Gil-mediated phospholipase C activity in the guinea pig cerebellum",

abstract = "PLC activity was stimulated either by 1-100 μM of GTP or by 100-3,000 μM Ca2+ in lysed synaptosomal membranes of the guinea pig cerebellum. The κ-opioid receptor agonist selectively inhibited the PLC activity stimulated by 100 μM GTP, but not by 100-3,000 μM Ca2+. Pretreatment of membranes with PTX abolished such a κ-agonist-induced inhibition of PLC activity. The reconstitution of Gi1, but not of Go purified from porcine brains with PTX-treated membranes showed a complete recovery of the κ-agonist-inhibition of PLC activity. These findings suggest that a novel subtype κ-receptor mediates inhibition of PLC through inhibiting the intrinsic activity of PTX-substrate G-proteins.",

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AU - Misawa, Hidemi

AU - Ueda, Hiroshi

AU - Katada, Toshiaki

AU - Ui, Michio

AU - Satoh, Masamichi

PY - 1995/3/13

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