A surgical technique using the ovarian vein in non-human primate models of potential living-donor surgery of uterus transplantation

Iori Kisu, Kouji Banno, Makoto Mihara, Hisako Hara, Kiyoko Umene, Masataka Adachi, Yuya Nogami, Daisuke Aoki

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Aim Living donor surgery in organ transplantation should be performed in a minimally invasive manner under conditions that are as safe as possible. The objective of this study is to examine whether the procedure for using the ovarian vein makes donor surgery less invasive in a cynomolgus monkey model of potential living-donor surgery of uterus transplantation. Material and methods Twenty-two female cynomolgus monkeys aged 6-9 years and with body weights of 3.55 ± 1.28 kg were used in the study. Vessels and tissues surrounding the uterus were dissected while preserving the uterine artery/vein. The deep uterine vein was used as a venous pedicle in four monkeys (Group 1), and the ovarian vein was used instead of the deep uterine vein in 18 monkeys (Group 2). With the uterine artery/vein and deep uterine vein (Group 1) or ovarian vein (Group 2) connected to the uterus, the vaginal canal was cut. The vessels were then clamped to produce a donor surgery model. Surgical time, intraoperative organ and vascular injury were examined in each animal. Results The average surgical time from laparotomy to clamping of vessels was 230 ± 112 min in all 22 cynomolgus monkeys, and significantly longer in Group 1 (n = 4) than in Group 2 (n = 18) (393 ± 71 vs. 194 ± 84 min, p < 0.05). Surgical time in Group 2 showed a tendency to decrease in animals treated later in the study, with a significantly longer time in the first 10 monkeys compared with the last 8 (253 ± 65 vs. 120 ± 26 min, p < 0.05). All monkeys had no complications, including no injuries to other organs and no unanticipated vascular injury. Conclusion The procedure using the ovarian vein was less invasive than that using the deep uterine vein in mimicking living-donor surgery in a cynomolgus monkey model of uterus transplantation.

Original languageEnglish
Pages (from-to)942-948
Number of pages7
JournalActa Obstetricia et Gynecologica Scandinavica
Volume94
Issue number9
DOIs
Publication statusPublished - 2015 Sep 1

Fingerprint

Living Donors
Primates
Uterus
Veins
Transplantation
Macaca fascicularis
Haplorhini
Operative Time
Uterine Artery
Vascular System Injuries
Tissue Donors
Organ Transplantation
Constriction
Laparotomy
Body Weight

Keywords

  • Cynomolgus monkey
  • donor surgery
  • invasive surgery
  • uterine factor infertility
  • uterus transplantation

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

A surgical technique using the ovarian vein in non-human primate models of potential living-donor surgery of uterus transplantation. / Kisu, Iori; Banno, Kouji; Mihara, Makoto; Hara, Hisako; Umene, Kiyoko; Adachi, Masataka; Nogami, Yuya; Aoki, Daisuke.

In: Acta Obstetricia et Gynecologica Scandinavica, Vol. 94, No. 9, 01.09.2015, p. 942-948.

Research output: Contribution to journalArticle

Kisu, Iori ; Banno, Kouji ; Mihara, Makoto ; Hara, Hisako ; Umene, Kiyoko ; Adachi, Masataka ; Nogami, Yuya ; Aoki, Daisuke. / A surgical technique using the ovarian vein in non-human primate models of potential living-donor surgery of uterus transplantation. In: Acta Obstetricia et Gynecologica Scandinavica. 2015 ; Vol. 94, No. 9. pp. 942-948.
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AU - Adachi, Masataka

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AB - Aim Living donor surgery in organ transplantation should be performed in a minimally invasive manner under conditions that are as safe as possible. The objective of this study is to examine whether the procedure for using the ovarian vein makes donor surgery less invasive in a cynomolgus monkey model of potential living-donor surgery of uterus transplantation. Material and methods Twenty-two female cynomolgus monkeys aged 6-9 years and with body weights of 3.55 ± 1.28 kg were used in the study. Vessels and tissues surrounding the uterus were dissected while preserving the uterine artery/vein. The deep uterine vein was used as a venous pedicle in four monkeys (Group 1), and the ovarian vein was used instead of the deep uterine vein in 18 monkeys (Group 2). With the uterine artery/vein and deep uterine vein (Group 1) or ovarian vein (Group 2) connected to the uterus, the vaginal canal was cut. The vessels were then clamped to produce a donor surgery model. Surgical time, intraoperative organ and vascular injury were examined in each animal. Results The average surgical time from laparotomy to clamping of vessels was 230 ± 112 min in all 22 cynomolgus monkeys, and significantly longer in Group 1 (n = 4) than in Group 2 (n = 18) (393 ± 71 vs. 194 ± 84 min, p < 0.05). Surgical time in Group 2 showed a tendency to decrease in animals treated later in the study, with a significantly longer time in the first 10 monkeys compared with the last 8 (253 ± 65 vs. 120 ± 26 min, p < 0.05). All monkeys had no complications, including no injuries to other organs and no unanticipated vascular injury. Conclusion The procedure using the ovarian vein was less invasive than that using the deep uterine vein in mimicking living-donor surgery in a cynomolgus monkey model of uterus transplantation.

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KW - uterine factor infertility

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