A temperate phage with cohesive ends induced by mitomycin C treatment of Lactobacillus casei: Brief report

Y. Nakashima, Hidetoshi Hasuwa, Y. Kakita, K. Murata, A. Kuroiwa, F. Miake, K. Watanabe

Research output: Contribution to journalArticle

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Abstract

A temperate phage, named PL-2, was induced from Lactobacillus casei ATCC 27092 by mitomycin C treatment of the cells at exponential growth phase. The phage had an isometric head of 45 nm in diameter and a flexible, noncontractile tail, 150 nm long and 10 nm wide, with a sharp tip. Along the tail axis, about 40 regularly spaced striae were seen. The phage DNA had complementary cohesive ends. The restriction enzyme map of the DNA was constructed by using 13 different restriction endonucleases. The size of the DNA was 35.2 kb, 83% in size of that of phage PL-1 lytic for the same Lb. casei strain.

Original languageEnglish
Pages (from-to)1621-1626
Number of pages6
JournalArchives of Virology
Volume143
Issue number8
DOIs
Publication statusPublished - 1998 Sep 21
Externally publishedYes

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Lactobacillus casei
Mitomycin
Bacteriophages
DNA Restriction Enzymes
Complementary DNA
Head
DNA
Growth

ASJC Scopus subject areas

  • Virology

Cite this

A temperate phage with cohesive ends induced by mitomycin C treatment of Lactobacillus casei : Brief report. / Nakashima, Y.; Hasuwa, Hidetoshi; Kakita, Y.; Murata, K.; Kuroiwa, A.; Miake, F.; Watanabe, K.

In: Archives of Virology, Vol. 143, No. 8, 21.09.1998, p. 1621-1626.

Research output: Contribution to journalArticle

Nakashima, Y. ; Hasuwa, Hidetoshi ; Kakita, Y. ; Murata, K. ; Kuroiwa, A. ; Miake, F. ; Watanabe, K. / A temperate phage with cohesive ends induced by mitomycin C treatment of Lactobacillus casei : Brief report. In: Archives of Virology. 1998 ; Vol. 143, No. 8. pp. 1621-1626.
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