A thin layer of sucrose octasulfate protects the oesophageal mucosal epithelium in reflux oesophagitis

Takuya Hayakawa, Shizuka Kawasaki, Yutaka Hirayama, Takuya Tsutsui, Eiji Sugiyama, Kiyo Adachi, Ryo Kon, Makoto Suematsu, Yuki Sugiura

Research output: Contribution to journalArticle

Abstract

Sucralfate is effective for the treatment of gastric and duodenal ulcers owing to its protective gel-forming ability. However, the mechanism by which sucralfate protects the oesophageal mucosa against reflux oesophagitis has not been clarified. We aimed to investigate the mechanisms of action of sucralfate and sucrose octasulfate (SOS), a component of sucralfate. SOS and sucralfate were administered to oesophagitis-induced rats, and the ulcer lesion size was macroscopically examined and scored. Effective pepsin activity in the gastric juices obtained from the animal model was evaluated by a casein digestion test. Sucralfate and SOS improved the pathology scores in a dose-dependent manner, whereas gastric juice pepsin activity was not impaired by therapeutic doses of SOS. As SOS lacks the ability to form a thick gel layer by polymerisation, we examined the distribution of SOS in the mucosal lumen by imaging mass spectrometry (IMS) to determine whether SOS directly adheres to the mucosal surface. A clear homogeneous thin-layer SOS film (>100 μm thick) was visualized on the oesophageal mucosal surface. Moreover, this SOS film formation was enhanced at ulcer lesion sites. Taken together, SOS appears to protect oesophageal mucosa against reflux oesophagitis via thin-layer formation on the mucosal surface.

Original languageEnglish
Article number3559
JournalScientific reports
Volume9
Issue number1
DOIs
Publication statusPublished - 2019 Dec 1

Fingerprint

Peptic Esophagitis
Sucralfate
Epithelium
Gastric Juice
Pepsin A
Ulcer
Gels
sucrose octasulfate
Esophagitis
Stomach Ulcer
Duodenal Ulcer
Caseins
Polymerization
Digestion
Mass Spectrometry
Animal Models
Pathology

ASJC Scopus subject areas

  • General

Cite this

A thin layer of sucrose octasulfate protects the oesophageal mucosal epithelium in reflux oesophagitis. / Hayakawa, Takuya; Kawasaki, Shizuka; Hirayama, Yutaka; Tsutsui, Takuya; Sugiyama, Eiji; Adachi, Kiyo; Kon, Ryo; Suematsu, Makoto; Sugiura, Yuki.

In: Scientific reports, Vol. 9, No. 1, 3559, 01.12.2019.

Research output: Contribution to journalArticle

Hayakawa, T, Kawasaki, S, Hirayama, Y, Tsutsui, T, Sugiyama, E, Adachi, K, Kon, R, Suematsu, M & Sugiura, Y 2019, 'A thin layer of sucrose octasulfate protects the oesophageal mucosal epithelium in reflux oesophagitis', Scientific reports, vol. 9, no. 1, 3559. https://doi.org/10.1038/s41598-019-39087-4
Hayakawa, Takuya ; Kawasaki, Shizuka ; Hirayama, Yutaka ; Tsutsui, Takuya ; Sugiyama, Eiji ; Adachi, Kiyo ; Kon, Ryo ; Suematsu, Makoto ; Sugiura, Yuki. / A thin layer of sucrose octasulfate protects the oesophageal mucosal epithelium in reflux oesophagitis. In: Scientific reports. 2019 ; Vol. 9, No. 1.
@article{8239d6cd184d443d980f1aa6e796ed33,
title = "A thin layer of sucrose octasulfate protects the oesophageal mucosal epithelium in reflux oesophagitis",
abstract = "Sucralfate is effective for the treatment of gastric and duodenal ulcers owing to its protective gel-forming ability. However, the mechanism by which sucralfate protects the oesophageal mucosa against reflux oesophagitis has not been clarified. We aimed to investigate the mechanisms of action of sucralfate and sucrose octasulfate (SOS), a component of sucralfate. SOS and sucralfate were administered to oesophagitis-induced rats, and the ulcer lesion size was macroscopically examined and scored. Effective pepsin activity in the gastric juices obtained from the animal model was evaluated by a casein digestion test. Sucralfate and SOS improved the pathology scores in a dose-dependent manner, whereas gastric juice pepsin activity was not impaired by therapeutic doses of SOS. As SOS lacks the ability to form a thick gel layer by polymerisation, we examined the distribution of SOS in the mucosal lumen by imaging mass spectrometry (IMS) to determine whether SOS directly adheres to the mucosal surface. A clear homogeneous thin-layer SOS film (>100 μm thick) was visualized on the oesophageal mucosal surface. Moreover, this SOS film formation was enhanced at ulcer lesion sites. Taken together, SOS appears to protect oesophageal mucosa against reflux oesophagitis via thin-layer formation on the mucosal surface.",
author = "Takuya Hayakawa and Shizuka Kawasaki and Yutaka Hirayama and Takuya Tsutsui and Eiji Sugiyama and Kiyo Adachi and Ryo Kon and Makoto Suematsu and Yuki Sugiura",
year = "2019",
month = "12",
day = "1",
doi = "10.1038/s41598-019-39087-4",
language = "English",
volume = "9",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - A thin layer of sucrose octasulfate protects the oesophageal mucosal epithelium in reflux oesophagitis

AU - Hayakawa, Takuya

AU - Kawasaki, Shizuka

AU - Hirayama, Yutaka

AU - Tsutsui, Takuya

AU - Sugiyama, Eiji

AU - Adachi, Kiyo

AU - Kon, Ryo

AU - Suematsu, Makoto

AU - Sugiura, Yuki

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Sucralfate is effective for the treatment of gastric and duodenal ulcers owing to its protective gel-forming ability. However, the mechanism by which sucralfate protects the oesophageal mucosa against reflux oesophagitis has not been clarified. We aimed to investigate the mechanisms of action of sucralfate and sucrose octasulfate (SOS), a component of sucralfate. SOS and sucralfate were administered to oesophagitis-induced rats, and the ulcer lesion size was macroscopically examined and scored. Effective pepsin activity in the gastric juices obtained from the animal model was evaluated by a casein digestion test. Sucralfate and SOS improved the pathology scores in a dose-dependent manner, whereas gastric juice pepsin activity was not impaired by therapeutic doses of SOS. As SOS lacks the ability to form a thick gel layer by polymerisation, we examined the distribution of SOS in the mucosal lumen by imaging mass spectrometry (IMS) to determine whether SOS directly adheres to the mucosal surface. A clear homogeneous thin-layer SOS film (>100 μm thick) was visualized on the oesophageal mucosal surface. Moreover, this SOS film formation was enhanced at ulcer lesion sites. Taken together, SOS appears to protect oesophageal mucosa against reflux oesophagitis via thin-layer formation on the mucosal surface.

AB - Sucralfate is effective for the treatment of gastric and duodenal ulcers owing to its protective gel-forming ability. However, the mechanism by which sucralfate protects the oesophageal mucosa against reflux oesophagitis has not been clarified. We aimed to investigate the mechanisms of action of sucralfate and sucrose octasulfate (SOS), a component of sucralfate. SOS and sucralfate were administered to oesophagitis-induced rats, and the ulcer lesion size was macroscopically examined and scored. Effective pepsin activity in the gastric juices obtained from the animal model was evaluated by a casein digestion test. Sucralfate and SOS improved the pathology scores in a dose-dependent manner, whereas gastric juice pepsin activity was not impaired by therapeutic doses of SOS. As SOS lacks the ability to form a thick gel layer by polymerisation, we examined the distribution of SOS in the mucosal lumen by imaging mass spectrometry (IMS) to determine whether SOS directly adheres to the mucosal surface. A clear homogeneous thin-layer SOS film (>100 μm thick) was visualized on the oesophageal mucosal surface. Moreover, this SOS film formation was enhanced at ulcer lesion sites. Taken together, SOS appears to protect oesophageal mucosa against reflux oesophagitis via thin-layer formation on the mucosal surface.

UR - http://www.scopus.com/inward/record.url?scp=85062584543&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85062584543&partnerID=8YFLogxK

U2 - 10.1038/s41598-019-39087-4

DO - 10.1038/s41598-019-39087-4

M3 - Article

C2 - 30837505

AN - SCOPUS:85062584543

VL - 9

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 3559

ER -