TY - GEN
T1 - A Three-Dimensional Artificial Intestinal Tissue With A Crypt-Like Inner Surface
AU - Tanaka, Shuma
AU - Itai, Shun
AU - Onoe, Hiroaki
N1 - Funding Information:
This work was partly supported by Mitochondrial preemptive medicine, MOONSHOT research & development program from Japan Agency for Medical Research and Development (AMED).
Publisher Copyright:
© 2023 IEEE.
PY - 2023
Y1 - 2023
N2 - Disruption of the microbiome in humans caused several diseases. To infer causality, the intestinal models are essential, but the commonly used model has still room for improvements such as biomimicry, productivity, and ability to co-culture with bacteria. This paper describes the artificial intestinal tissue by culturing intestinal cells (Caco-2) in the collagen gel tube with crypt-like cavities created by electrolysis-triggered microbubbles. This tube device has high biomimicry and productivity and also consists of glass/silicone tubes connector at both ends, which allows for connecting external pumps easily and running two types of flows: the inside of the tube (internal flow) and permeation from the outside of the tube to the inside (external flow). In order to show this high extensibility, we demonstrated the co-culture of Caco-2 cells and bacteria (Bifidobacteria). We believe that our device will be a platform for investigating complex intestinal diseases due to its high biomimetic properties.
AB - Disruption of the microbiome in humans caused several diseases. To infer causality, the intestinal models are essential, but the commonly used model has still room for improvements such as biomimicry, productivity, and ability to co-culture with bacteria. This paper describes the artificial intestinal tissue by culturing intestinal cells (Caco-2) in the collagen gel tube with crypt-like cavities created by electrolysis-triggered microbubbles. This tube device has high biomimicry and productivity and also consists of glass/silicone tubes connector at both ends, which allows for connecting external pumps easily and running two types of flows: the inside of the tube (internal flow) and permeation from the outside of the tube to the inside (external flow). In order to show this high extensibility, we demonstrated the co-culture of Caco-2 cells and bacteria (Bifidobacteria). We believe that our device will be a platform for investigating complex intestinal diseases due to its high biomimetic properties.
KW - Bacteria
KW - Cell culture
KW - Co-culture
KW - Collagen gel tube
KW - Electrolysis
KW - Intestinal tissue
KW - Intestine
KW - Organoid
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U2 - 10.1109/MEMS49605.2023.10052541
DO - 10.1109/MEMS49605.2023.10052541
M3 - Conference contribution
AN - SCOPUS:85149848219
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 456
EP - 459
BT - 2023 IEEE 36th International Conference on Micro Electro Mechanical Systems, MEMS 2023
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 36th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2023
Y2 - 15 January 2023 through 19 January 2023
ER -