A tissue microRNA signature that predicts the prognosis of breast cancer in young women

Ai Hironaka-Mitsuhashi, Juntaro Matsuzaki, Ryou U. Takahashi, Masayuki Yoshida, Yutaka Nezu, Yusuke Yamamoto, Sho Shiino, Takayuki Kinoshita, Toshikazu Ushijima, Nobuyoshi Hiraoka, Chikako Shimizu, Kenji Tamura, Takahiro Ochiya

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Since breast cancers in young women are generally aggressive, young patients tend to be intensively treated with anti-cancer drugs. To optimize the strategy for treatment, particularly in young women, prognostic biomarkers are urgently required. The objective of this study was to identify a tissue microRNA (miRNA) signature that predicts prognosis in young breast cancer patients. Total RNA from 45 breast cancer patients aged <35 years was extracted from formalin-fixed paraffin-embedded (FFPE) tissues and analyzed using miRNA microarrays. Patients were categorized into two groups according to recurrence status within the 5 year period after surgery: recurrence (n = 11) and non-recurrent (n = 34). Histological parameters of hormone receptors and Ki-67 were statistically compared between the two groups. Differentially expressed miRNAs were identified, and their associations with overall survival (OS) were evaluated by log-rank test. The median observation period was 5.8 years for the recurrent group, and 9.1 years for the non-recurrent group. Nine miRNAs were significantly differentially expressed between the recurrent and non-recurrent groups. Receiver Operating Characteristic curve analysis was performed to evaluate the prediction accuracy of the identified miRNAs, and the resultant area under the curve was >0.7. Five of the miRNAs were validated by qRT-PCR, and the expression levels of three of those five (miR-183-5p, miR-194-5p, and miR-1285-5p), both alone and in combination, were associated with OS. In conclusion, we identified three candidate miRNAs that could be used separately or in combination as prognostic biomarkers in young breast cancer patients. This miRNA signature may enable selection of better treatment choices for young women with this disease.

Original languageEnglish
Article numbere0187638
JournalPloS one
Volume12
Issue number11
DOIs
Publication statusPublished - 2017 Nov 1
Externally publishedYes

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MicroRNAs
microRNA
breast neoplasms
prognosis
Tissue
Breast Neoplasms
Biomarkers
biomarkers
antineoplastic agents
RNA
Polymerase Chain Reaction
tissues
Therapeutics
Pharmaceutical Preparations
Neoplasms

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Hironaka-Mitsuhashi, A., Matsuzaki, J., Takahashi, R. U., Yoshida, M., Nezu, Y., Yamamoto, Y., ... Ochiya, T. (2017). A tissue microRNA signature that predicts the prognosis of breast cancer in young women. PloS one, 12(11), [e0187638]. https://doi.org/10.1371/journal.pone.0187638

A tissue microRNA signature that predicts the prognosis of breast cancer in young women. / Hironaka-Mitsuhashi, Ai; Matsuzaki, Juntaro; Takahashi, Ryou U.; Yoshida, Masayuki; Nezu, Yutaka; Yamamoto, Yusuke; Shiino, Sho; Kinoshita, Takayuki; Ushijima, Toshikazu; Hiraoka, Nobuyoshi; Shimizu, Chikako; Tamura, Kenji; Ochiya, Takahiro.

In: PloS one, Vol. 12, No. 11, e0187638, 01.11.2017.

Research output: Contribution to journalArticle

Hironaka-Mitsuhashi, A, Matsuzaki, J, Takahashi, RU, Yoshida, M, Nezu, Y, Yamamoto, Y, Shiino, S, Kinoshita, T, Ushijima, T, Hiraoka, N, Shimizu, C, Tamura, K & Ochiya, T 2017, 'A tissue microRNA signature that predicts the prognosis of breast cancer in young women', PloS one, vol. 12, no. 11, e0187638. https://doi.org/10.1371/journal.pone.0187638
Hironaka-Mitsuhashi A, Matsuzaki J, Takahashi RU, Yoshida M, Nezu Y, Yamamoto Y et al. A tissue microRNA signature that predicts the prognosis of breast cancer in young women. PloS one. 2017 Nov 1;12(11). e0187638. https://doi.org/10.1371/journal.pone.0187638
Hironaka-Mitsuhashi, Ai ; Matsuzaki, Juntaro ; Takahashi, Ryou U. ; Yoshida, Masayuki ; Nezu, Yutaka ; Yamamoto, Yusuke ; Shiino, Sho ; Kinoshita, Takayuki ; Ushijima, Toshikazu ; Hiraoka, Nobuyoshi ; Shimizu, Chikako ; Tamura, Kenji ; Ochiya, Takahiro. / A tissue microRNA signature that predicts the prognosis of breast cancer in young women. In: PloS one. 2017 ; Vol. 12, No. 11.
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