A transcript map of the candidate genes in the down syndrome critical region of human chromosome 21

Nobuvoshi Shimizu, Nobuaki Shindoh, Hideto Maeda, Akiko Yamakf, Kazuhiko Kawasaki, Shuichi Asakawa, Kazutoyo Osoegawa, Eiichi Soeda, Yoshiko Shimizu, Shinsei Minoshima, Jun Kudo

Research output: Contribution to journalArticle

Abstract

To isolate genes potentially involved in the pathogenesis of Down syndrome, we have performed exon trapping for the "Down syndrome critical region". We have isolated over 100 exons including those from known human genes and homologs of Drosophila singleminded (sim) and minibrain (mnb) genes. These genes locate in the order of cen-SIM2HCS-TPRD-MNB-KCNJ6-ERG-tel. Drosophila sim and mnb genes are required for development of central nervous system. The structural characteristics of human and mouse SIM2 proteins that contain bHLH and PAS domains and expression in the diencephalon during mouse embryogenesis strongly suggest that SIM proteins function as a transcriptional regulator in the development of the central nervous system. Human MNB cDNA encodes a protein kinase with a nuclear targeting signal and a catalytic domain highly homologous to Drosophila mnb protein kinase and strikingly resembles the rat Dyrk protein kinase with a dual specificity. The MNB mRNA is expressed in various tissues including fetal and adult brains. These results implicate that human MNB protein may play a significant role in a signaling pathway regulating nuclear functions of neuronal cell proliferation, contributing to certain features of Down syndrome.

Original languageEnglish
Pages (from-to)84
Number of pages1
JournalJapanese Journal of Human Genetics
Volume42
Issue number1
Publication statusPublished - 1997

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Chromosomes, Human, Pair 21
Human Chromosomes
Protein Kinases
Genes
Down Syndrome
Drosophila
Exons
Central Nervous System
Diencephalon
Embryonic Development
Catalytic Domain
Proteins
Fetus
Complementary DNA
Cell Proliferation
Down Syndrome Critical Region
Messenger RNA
Brain

ASJC Scopus subject areas

  • Genetics(clinical)

Cite this

Shimizu, N., Shindoh, N., Maeda, H., Yamakf, A., Kawasaki, K., Asakawa, S., ... Kudo, J. (1997). A transcript map of the candidate genes in the down syndrome critical region of human chromosome 21. Japanese Journal of Human Genetics, 42(1), 84.

A transcript map of the candidate genes in the down syndrome critical region of human chromosome 21. / Shimizu, Nobuvoshi; Shindoh, Nobuaki; Maeda, Hideto; Yamakf, Akiko; Kawasaki, Kazuhiko; Asakawa, Shuichi; Osoegawa, Kazutoyo; Soeda, Eiichi; Shimizu, Yoshiko; Minoshima, Shinsei; Kudo, Jun.

In: Japanese Journal of Human Genetics, Vol. 42, No. 1, 1997, p. 84.

Research output: Contribution to journalArticle

Shimizu, N, Shindoh, N, Maeda, H, Yamakf, A, Kawasaki, K, Asakawa, S, Osoegawa, K, Soeda, E, Shimizu, Y, Minoshima, S & Kudo, J 1997, 'A transcript map of the candidate genes in the down syndrome critical region of human chromosome 21', Japanese Journal of Human Genetics, vol. 42, no. 1, pp. 84.
Shimizu, Nobuvoshi ; Shindoh, Nobuaki ; Maeda, Hideto ; Yamakf, Akiko ; Kawasaki, Kazuhiko ; Asakawa, Shuichi ; Osoegawa, Kazutoyo ; Soeda, Eiichi ; Shimizu, Yoshiko ; Minoshima, Shinsei ; Kudo, Jun. / A transcript map of the candidate genes in the down syndrome critical region of human chromosome 21. In: Japanese Journal of Human Genetics. 1997 ; Vol. 42, No. 1. pp. 84.
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