A transcriptome derived female-specific marker from the invasive western mosquitofish (Gambusia affinis)

Dunja K. Lamatsch, Sofia Adolfsson, Alistair M. Senior, Guntram Christiansen, Maria Pichler, Yuichi Ozaki, Linnea Smeds, Manfred Schartl, Shinichi Nakagawa

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Sex-specific markers are a prerequisite for understanding reproductive biology, genetic factors involved in sex differences, mechanisms of sex determination, and ultimately the evolution of sex chromosomes. The Western mosquitofish, Gambusia affinis, may be considered a model species for sex-chromosome evolution, as it displays female heterogamety (ZW/ZZ), and is also ecologically interesting as a worldwide invasive species. Here, de novo RNA-sequencing on the gonads of sexually mature G. affinis was used to identify contigs that were highly transcribed in females but not in males (i.e., transcripts with ovary-specific expression). Subsequently, 129 primer pairs spanning 79 contigs were tested by PCR to identify sex-specific transcripts. Of those primer pairs, one female-specific DNA marker was identified, Sanger sequenced and subsequently validated in 115 fish. Sequence analyses revealed a high similarity between the identified sex-specific marker and the 3′ UTR of the aminomethyl transferase (amt) gene of the closely related platyfish (Xiphophorus maculatus). This is the first time that RNA-seq has been used to successfully characterize a sexspecific marker in a fish species in the absence of a genome map. Additionally, the identified sex-specific marker represents one of only a handful of such markers in fishes.

Original languageEnglish
Article numbere0118214
JournalPLoS One
Volume10
Issue number2
DOIs
Publication statusPublished - 2015 Feb 23
Externally publishedYes

Fingerprint

Cyprinodontiformes
Gambusia affinis
Transcriptome
transcriptome
Fish
gender
Genes
Fishes
Sex Chromosomes
RNA
sex chromosomes
3' Untranslated Regions
Transferases
fish
RNA Sequence Analysis
Introduced Species
Gonads
3' untranslated regions
Genetic Markers
transferases

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Lamatsch, D. K., Adolfsson, S., Senior, A. M., Christiansen, G., Pichler, M., Ozaki, Y., ... Nakagawa, S. (2015). A transcriptome derived female-specific marker from the invasive western mosquitofish (Gambusia affinis). PLoS One, 10(2), [e0118214]. https://doi.org/10.1371/journal.pone.0118214

A transcriptome derived female-specific marker from the invasive western mosquitofish (Gambusia affinis). / Lamatsch, Dunja K.; Adolfsson, Sofia; Senior, Alistair M.; Christiansen, Guntram; Pichler, Maria; Ozaki, Yuichi; Smeds, Linnea; Schartl, Manfred; Nakagawa, Shinichi.

In: PLoS One, Vol. 10, No. 2, e0118214, 23.02.2015.

Research output: Contribution to journalArticle

Lamatsch, DK, Adolfsson, S, Senior, AM, Christiansen, G, Pichler, M, Ozaki, Y, Smeds, L, Schartl, M & Nakagawa, S 2015, 'A transcriptome derived female-specific marker from the invasive western mosquitofish (Gambusia affinis)', PLoS One, vol. 10, no. 2, e0118214. https://doi.org/10.1371/journal.pone.0118214
Lamatsch DK, Adolfsson S, Senior AM, Christiansen G, Pichler M, Ozaki Y et al. A transcriptome derived female-specific marker from the invasive western mosquitofish (Gambusia affinis). PLoS One. 2015 Feb 23;10(2). e0118214. https://doi.org/10.1371/journal.pone.0118214
Lamatsch, Dunja K. ; Adolfsson, Sofia ; Senior, Alistair M. ; Christiansen, Guntram ; Pichler, Maria ; Ozaki, Yuichi ; Smeds, Linnea ; Schartl, Manfred ; Nakagawa, Shinichi. / A transcriptome derived female-specific marker from the invasive western mosquitofish (Gambusia affinis). In: PLoS One. 2015 ; Vol. 10, No. 2.
@article{7e3563c7edc94d8db9248503554860a5,
title = "A transcriptome derived female-specific marker from the invasive western mosquitofish (Gambusia affinis)",
abstract = "Sex-specific markers are a prerequisite for understanding reproductive biology, genetic factors involved in sex differences, mechanisms of sex determination, and ultimately the evolution of sex chromosomes. The Western mosquitofish, Gambusia affinis, may be considered a model species for sex-chromosome evolution, as it displays female heterogamety (ZW/ZZ), and is also ecologically interesting as a worldwide invasive species. Here, de novo RNA-sequencing on the gonads of sexually mature G. affinis was used to identify contigs that were highly transcribed in females but not in males (i.e., transcripts with ovary-specific expression). Subsequently, 129 primer pairs spanning 79 contigs were tested by PCR to identify sex-specific transcripts. Of those primer pairs, one female-specific DNA marker was identified, Sanger sequenced and subsequently validated in 115 fish. Sequence analyses revealed a high similarity between the identified sex-specific marker and the 3′ UTR of the aminomethyl transferase (amt) gene of the closely related platyfish (Xiphophorus maculatus). This is the first time that RNA-seq has been used to successfully characterize a sexspecific marker in a fish species in the absence of a genome map. Additionally, the identified sex-specific marker represents one of only a handful of such markers in fishes.",
author = "Lamatsch, {Dunja K.} and Sofia Adolfsson and Senior, {Alistair M.} and Guntram Christiansen and Maria Pichler and Yuichi Ozaki and Linnea Smeds and Manfred Schartl and Shinichi Nakagawa",
year = "2015",
month = "2",
day = "23",
doi = "10.1371/journal.pone.0118214",
language = "English",
volume = "10",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "2",

}

TY - JOUR

T1 - A transcriptome derived female-specific marker from the invasive western mosquitofish (Gambusia affinis)

AU - Lamatsch, Dunja K.

AU - Adolfsson, Sofia

AU - Senior, Alistair M.

AU - Christiansen, Guntram

AU - Pichler, Maria

AU - Ozaki, Yuichi

AU - Smeds, Linnea

AU - Schartl, Manfred

AU - Nakagawa, Shinichi

PY - 2015/2/23

Y1 - 2015/2/23

N2 - Sex-specific markers are a prerequisite for understanding reproductive biology, genetic factors involved in sex differences, mechanisms of sex determination, and ultimately the evolution of sex chromosomes. The Western mosquitofish, Gambusia affinis, may be considered a model species for sex-chromosome evolution, as it displays female heterogamety (ZW/ZZ), and is also ecologically interesting as a worldwide invasive species. Here, de novo RNA-sequencing on the gonads of sexually mature G. affinis was used to identify contigs that were highly transcribed in females but not in males (i.e., transcripts with ovary-specific expression). Subsequently, 129 primer pairs spanning 79 contigs were tested by PCR to identify sex-specific transcripts. Of those primer pairs, one female-specific DNA marker was identified, Sanger sequenced and subsequently validated in 115 fish. Sequence analyses revealed a high similarity between the identified sex-specific marker and the 3′ UTR of the aminomethyl transferase (amt) gene of the closely related platyfish (Xiphophorus maculatus). This is the first time that RNA-seq has been used to successfully characterize a sexspecific marker in a fish species in the absence of a genome map. Additionally, the identified sex-specific marker represents one of only a handful of such markers in fishes.

AB - Sex-specific markers are a prerequisite for understanding reproductive biology, genetic factors involved in sex differences, mechanisms of sex determination, and ultimately the evolution of sex chromosomes. The Western mosquitofish, Gambusia affinis, may be considered a model species for sex-chromosome evolution, as it displays female heterogamety (ZW/ZZ), and is also ecologically interesting as a worldwide invasive species. Here, de novo RNA-sequencing on the gonads of sexually mature G. affinis was used to identify contigs that were highly transcribed in females but not in males (i.e., transcripts with ovary-specific expression). Subsequently, 129 primer pairs spanning 79 contigs were tested by PCR to identify sex-specific transcripts. Of those primer pairs, one female-specific DNA marker was identified, Sanger sequenced and subsequently validated in 115 fish. Sequence analyses revealed a high similarity between the identified sex-specific marker and the 3′ UTR of the aminomethyl transferase (amt) gene of the closely related platyfish (Xiphophorus maculatus). This is the first time that RNA-seq has been used to successfully characterize a sexspecific marker in a fish species in the absence of a genome map. Additionally, the identified sex-specific marker represents one of only a handful of such markers in fishes.

UR - http://www.scopus.com/inward/record.url?scp=84923308614&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84923308614&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0118214

DO - 10.1371/journal.pone.0118214

M3 - Article

AN - SCOPUS:84923308614

VL - 10

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 2

M1 - e0118214

ER -