A transient parabiosis skin transplantation model in mice

Annique M M J Duyverman, Mitsutomo Kohno, Dan G. Duda, Rakesh K. Jain, Dai Fukumura

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Parabiosis-conjoined surgery to provide a shared circulation between two mice-has been previously developed to study the hematopoietic system. This protocol describes the use of parabiosis for efficient transplantation of skin from a transgenic to a wild-type mouse. It can be used to study the role of stromal cells in a spontaneous model of distant cancer dissemination (metastasis). We have recently shown that primary tumor-derived stromal cells may facilitate metastasis by providing a provisional stroma at the secondary site. Studying the role of primary tumor-derived stroma cells requires methods for distinguishing and targeting stromal cells originating from the primary tumor versus their counterparts in the metastatic site. Parabiosis may also be used, taking advantage of the shared circulation between the parabiosed mice, to study tumor metastasis from one parabiont to another, or to investigate the role of circulating inflammatory cells or stem cells. Studying the role of stromal cells in metastasis using this model typically takes up to 11 weeks.

Original languageEnglish
Pages (from-to)763-770
Number of pages8
JournalNature Protocols
Volume7
Issue number4
DOIs
Publication statusPublished - 2012 Apr

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Parabiosis
Skin Transplantation
Tumors
Skin
Stromal Cells
Neoplasm Metastasis
Neoplasms
Hematopoietic System
Stem cells
Surgery
Stem Cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Duyverman, A. M. M. J., Kohno, M., Duda, D. G., Jain, R. K., & Fukumura, D. (2012). A transient parabiosis skin transplantation model in mice. Nature Protocols, 7(4), 763-770. https://doi.org/10.1038/nprot.2012.032

A transient parabiosis skin transplantation model in mice. / Duyverman, Annique M M J; Kohno, Mitsutomo; Duda, Dan G.; Jain, Rakesh K.; Fukumura, Dai.

In: Nature Protocols, Vol. 7, No. 4, 04.2012, p. 763-770.

Research output: Contribution to journalArticle

Duyverman, AMMJ, Kohno, M, Duda, DG, Jain, RK & Fukumura, D 2012, 'A transient parabiosis skin transplantation model in mice', Nature Protocols, vol. 7, no. 4, pp. 763-770. https://doi.org/10.1038/nprot.2012.032
Duyverman AMMJ, Kohno M, Duda DG, Jain RK, Fukumura D. A transient parabiosis skin transplantation model in mice. Nature Protocols. 2012 Apr;7(4):763-770. https://doi.org/10.1038/nprot.2012.032
Duyverman, Annique M M J ; Kohno, Mitsutomo ; Duda, Dan G. ; Jain, Rakesh K. ; Fukumura, Dai. / A transient parabiosis skin transplantation model in mice. In: Nature Protocols. 2012 ; Vol. 7, No. 4. pp. 763-770.
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