A two-step sandwich enzyme immunoassay for rabbit proMMP-3 using monoclonal antibodies

Y. Morita, K. Igeta, E. Azumano, T. Kunisawa, K. Iwata, Y. Okada, A. Ito, Y. Mori, H. Nagase

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3 Citations (Scopus)

Abstract

A two-step sandwich enzyme immunoassay (EIA) system for the precursor of rabbit MMP-3 (r-proMMP-3) using monoclonal antibodies has been established to quantify the proMMP-3 levels in t he rabbit body fluids. The EIA system takes 150 min for the reactions (120 min for the first and second immunoreactions and 30 min for color reaction at 20°C). Sensitivity for rabbit proMMP-3 was 4.7 ng/ml (140 pg/assay) and linearity was obtained from 17 1,067 ng/ml (0.5 32 ng/assay). The EIA system recognizes only proMMP-3, but not its reactive forms. The levels of proMMP-3 (111 ± 9.3 ng/ml) in the synovial fluids from rabbit osteoarthritic models were higher than those from the normal control animals.

Original languageEnglish
Pages (from-to)183-190
Number of pages8
JournalConnective Tissue
Volume27
Issue number3-4
Publication statusPublished - 1995 Dec 1

Keywords

  • monoclonal antibodies
  • rabbit proMMP-3
  • sandwich enzyme immunoassay

ASJC Scopus subject areas

  • Rheumatology

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    Morita, Y., Igeta, K., Azumano, E., Kunisawa, T., Iwata, K., Okada, Y., Ito, A., Mori, Y., & Nagase, H. (1995). A two-step sandwich enzyme immunoassay for rabbit proMMP-3 using monoclonal antibodies. Connective Tissue, 27(3-4), 183-190.