A typical Y1 receptor regulates feeding behaviors: Effects of a potent and selective Y1 antagonist, J-115814

Akio Kanatani, Mikiko Hata, Satoshi Mashiko, Akane Ishihara, Osamu Okamoto, Yuji Haga, Tomoyuki Ohe, Tetsuya Kanno, Naomi Murai, Yasuyuki Ishii, Takahiro Fukuroda, Takehiro Fukami, Masaki Ihara

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90 Citations (Scopus)

Abstract

Neuropeptide Y (NPY) is a potent feeding stimulant. The orexigenic effect of NPY might be caused in part by the action of Y1 receptors. However, the existence of multiple NPY receptors including a possible novel feeding receptor has made it difficult to determine the relative importance of the Y1 receptor in feeding regulation. Herein we certified that the Y1 receptor is a major feeding receptor of NPY by using the potent and selective Y1 antagonist (-)-2-[1-(3-chloro-5isopropyloxycarbon-ylaminophenyl)ethylamino]-6-[2-(5-ethy l-4-methyl-1,3-thiazol-2-yl)ethyl]-4-morpholinopyridine (J-115814) and Y1 receptordeficient (Y1-/-) mice. J-115814 displaced 125l-peptide YY binding to cell membranes expressing cloned human, rat, and murine Y1 receptors with Ki values of 1.4, 1.8, and 1.9 nM, respectively, and inhibited NPY (10 nM)-induced increases in intracellular calcium levels via human Y1 receptors (IC50 = 6.8 nM). In contrast, J-115814 showed low affinities for human Y2 (Ki > 10 μM), Y4 (Ki = 640 nM) and Y5 receptors (Ki = 6000 nM). Intracerebroventricular (ICV) (10-100 μg) and intravenous (IV) (0.3-30 mg/kg) administration of J-115814 significantly and dose-dependently suppressed feeding induced by ICV NPY (5 μg) in satiated Sprague-Dawley rats. Intraperitoneal (IP) administration of J-115814 (3-30 mg/kg) significantly attenuated spontaneous feeding in db/db and C57BL6 mice. Feeding induced by ICV NPY (5 μg) was unaffected by IP-injected J-115814 (30 mg/kg) in Y1-/- mice and was suppressed in wild-type and Y5-/- mice. These findings clearly suggest that J-115814 inhibits feeding behaviors through the inhibition of the typical Y1 receptor. We conclude that the Y1 receptor plays a key role in regulating food intake.

Original languageEnglish
Pages (from-to)501-505
Number of pages5
JournalMolecular Pharmacology
Volume59
Issue number3
DOIs
Publication statusPublished - 2001 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

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    Kanatani, A., Hata, M., Mashiko, S., Ishihara, A., Okamoto, O., Haga, Y., Ohe, T., Kanno, T., Murai, N., Ishii, Y., Fukuroda, T., Fukami, T., & Ihara, M. (2001). A typical Y1 receptor regulates feeding behaviors: Effects of a potent and selective Y1 antagonist, J-115814. Molecular Pharmacology, 59(3), 501-505. https://doi.org/10.1124/mol.59.3.501