A web-based interactive developmental table for the Ascidian Ciona intestinalis, including 3D real-image embryo reconstructions

I. From fertilized egg to hatching larva

Kohji Hotta, Kenta Mitsuhara, Hiroki Takahashi, Kazuo Inaba, Kotaro Oka, Takashi Gojobori, Kazuho Ikeo

Research output: Contribution to journalArticle

127 Citations (Scopus)

Abstract

The ascidian chordate Ciona intestinalis is an established model organism frequently exploited to examine cellular development and a rapidly emerging model organism with a strong potential for developmental systems biology studies. However, there is no standardized developmental table for this organism. In this study, we made the standard web-based image resource called FABA: Four-dimensional Ascidian Body Atlas including ascidian's three-dimensional (3D) and cross-sectional images through the developmental time course. These images were reconstructed from more than 3,000 high-resolution real images collected by confocal laser scanning microscopy (CLSM) at newly defined 26 distinct developmental stages (stages 1-26) from fertilized egg to hatching larva, which were grouped into six periods named the zygote, cleavage, gastrula, neurula, tailbud, and larva periods. Our data set will be helpful in standardizing developmental stages for morphology comparison as well as for providing the guideline for several functional studies of a body plan in chordate.

Original languageEnglish
Pages (from-to)1790-1805
Number of pages16
JournalDevelopmental Dynamics
Volume236
Issue number7
DOIs
Publication statusPublished - 2007 Jul

Fingerprint

Ciona intestinalis
Urochordata
Computer-Assisted Image Processing
Zygote
Chordata
Larva
Embryonic Structures
Gastrula
Developmental Biology
Systems Biology
Atlases
Confocal Microscopy
Guidelines

Keywords

  • 3D
  • Ascidian
  • Body atlas
  • Ciona
  • CLSM
  • Database
  • Developmental stage
  • Morphology
  • Spatio-temporal
  • Time-lapse

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

A web-based interactive developmental table for the Ascidian Ciona intestinalis, including 3D real-image embryo reconstructions : I. From fertilized egg to hatching larva. / Hotta, Kohji; Mitsuhara, Kenta; Takahashi, Hiroki; Inaba, Kazuo; Oka, Kotaro; Gojobori, Takashi; Ikeo, Kazuho.

In: Developmental Dynamics, Vol. 236, No. 7, 07.2007, p. 1790-1805.

Research output: Contribution to journalArticle

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