Aberrant expression of β-catenin and mutation of exon 3 of the β-catenin gene in renal and urothelial carcinomas

Xudong Zhu, Yae Kanai, Atsushi Saito, Yutaka Kondo, Setsuo Hirohashi

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The present study attempted to clarify the significance of aberrant expression of β-catenin protein and mutation of exon 3 of the β-catenin gene in renal and urothelial carcinogenesis. β-Catenin expression was examined immunohistochemically and mutation of the β-catenin gene was analyzed by polymerase chain reaction-single strand conformation polymorphism (SSCP) and direct sequencing. β-Catenin immunoreactivity was observed at the cell membrane in all 30 renal cell carcinomas (RCC) examined, and no RCC showed a mobility-shifted SSCP band. Of 46 transitional cell carcinomas (TCC) examined, there was reduced expression of β-catenin, as compared with its expression in non-cancerous transitional epithelium, in 22 cases (48%) and catenin accumulation in the nucleus in five cases (11%). Of four renal pelvis TCC examined, point mutation of exon 3 of the β-catenin gene at codon 45 resulting in amino acid substitution (Ser to Phe) was detected in one (25%). The incidence of reduced expression of β-catenin correlated significantly with the growth pattern (superficial type vs invasive type) of TCC (P < 0.05). These data indicate that: (1) aberrant β-catenin expression may be at least partly involved in urothelial carcinogenesis, but less significantly so in renal carcinogenesis, and (2) it may be associated with the progression of TCC showing invasive growth.

Original languageEnglish
Pages (from-to)945-952
Number of pages8
JournalPathology International
Volume50
Issue number12
DOIs
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Catenins
Exons
Carcinoma
Kidney
Mutation
Transitional Cell Carcinoma
Genes
Carcinogenesis
Renal Cell Carcinoma
Kidney Pelvis
Amino Acid Substitution
Growth
Point Mutation
Codon
Epithelium
Cell Membrane

Keywords

  • β-catenin
  • Renal cell carcinoma
  • Renal pelvis
  • Single strand conformation polymorphism
  • Transitional cell carcinoma
  • Urinary bladder

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Aberrant expression of β-catenin and mutation of exon 3 of the β-catenin gene in renal and urothelial carcinomas. / Zhu, Xudong; Kanai, Yae; Saito, Atsushi; Kondo, Yutaka; Hirohashi, Setsuo.

In: Pathology International, Vol. 50, No. 12, 2000, p. 945-952.

Research output: Contribution to journalArticle

Zhu, Xudong ; Kanai, Yae ; Saito, Atsushi ; Kondo, Yutaka ; Hirohashi, Setsuo. / Aberrant expression of β-catenin and mutation of exon 3 of the β-catenin gene in renal and urothelial carcinomas. In: Pathology International. 2000 ; Vol. 50, No. 12. pp. 945-952.
@article{b2e4ba02424c445d85ae5e450f2b21b0,
title = "Aberrant expression of β-catenin and mutation of exon 3 of the β-catenin gene in renal and urothelial carcinomas",
abstract = "The present study attempted to clarify the significance of aberrant expression of β-catenin protein and mutation of exon 3 of the β-catenin gene in renal and urothelial carcinogenesis. β-Catenin expression was examined immunohistochemically and mutation of the β-catenin gene was analyzed by polymerase chain reaction-single strand conformation polymorphism (SSCP) and direct sequencing. β-Catenin immunoreactivity was observed at the cell membrane in all 30 renal cell carcinomas (RCC) examined, and no RCC showed a mobility-shifted SSCP band. Of 46 transitional cell carcinomas (TCC) examined, there was reduced expression of β-catenin, as compared with its expression in non-cancerous transitional epithelium, in 22 cases (48{\%}) and catenin accumulation in the nucleus in five cases (11{\%}). Of four renal pelvis TCC examined, point mutation of exon 3 of the β-catenin gene at codon 45 resulting in amino acid substitution (Ser to Phe) was detected in one (25{\%}). The incidence of reduced expression of β-catenin correlated significantly with the growth pattern (superficial type vs invasive type) of TCC (P < 0.05). These data indicate that: (1) aberrant β-catenin expression may be at least partly involved in urothelial carcinogenesis, but less significantly so in renal carcinogenesis, and (2) it may be associated with the progression of TCC showing invasive growth.",
keywords = "β-catenin, Renal cell carcinoma, Renal pelvis, Single strand conformation polymorphism, Transitional cell carcinoma, Urinary bladder",
author = "Xudong Zhu and Yae Kanai and Atsushi Saito and Yutaka Kondo and Setsuo Hirohashi",
year = "2000",
doi = "10.1046/j.1440-1827.2000.01139.x",
language = "English",
volume = "50",
pages = "945--952",
journal = "Pathology International",
issn = "1320-5463",
publisher = "Wiley-Blackwell",
number = "12",

}

TY - JOUR

T1 - Aberrant expression of β-catenin and mutation of exon 3 of the β-catenin gene in renal and urothelial carcinomas

AU - Zhu, Xudong

AU - Kanai, Yae

AU - Saito, Atsushi

AU - Kondo, Yutaka

AU - Hirohashi, Setsuo

PY - 2000

Y1 - 2000

N2 - The present study attempted to clarify the significance of aberrant expression of β-catenin protein and mutation of exon 3 of the β-catenin gene in renal and urothelial carcinogenesis. β-Catenin expression was examined immunohistochemically and mutation of the β-catenin gene was analyzed by polymerase chain reaction-single strand conformation polymorphism (SSCP) and direct sequencing. β-Catenin immunoreactivity was observed at the cell membrane in all 30 renal cell carcinomas (RCC) examined, and no RCC showed a mobility-shifted SSCP band. Of 46 transitional cell carcinomas (TCC) examined, there was reduced expression of β-catenin, as compared with its expression in non-cancerous transitional epithelium, in 22 cases (48%) and catenin accumulation in the nucleus in five cases (11%). Of four renal pelvis TCC examined, point mutation of exon 3 of the β-catenin gene at codon 45 resulting in amino acid substitution (Ser to Phe) was detected in one (25%). The incidence of reduced expression of β-catenin correlated significantly with the growth pattern (superficial type vs invasive type) of TCC (P < 0.05). These data indicate that: (1) aberrant β-catenin expression may be at least partly involved in urothelial carcinogenesis, but less significantly so in renal carcinogenesis, and (2) it may be associated with the progression of TCC showing invasive growth.

AB - The present study attempted to clarify the significance of aberrant expression of β-catenin protein and mutation of exon 3 of the β-catenin gene in renal and urothelial carcinogenesis. β-Catenin expression was examined immunohistochemically and mutation of the β-catenin gene was analyzed by polymerase chain reaction-single strand conformation polymorphism (SSCP) and direct sequencing. β-Catenin immunoreactivity was observed at the cell membrane in all 30 renal cell carcinomas (RCC) examined, and no RCC showed a mobility-shifted SSCP band. Of 46 transitional cell carcinomas (TCC) examined, there was reduced expression of β-catenin, as compared with its expression in non-cancerous transitional epithelium, in 22 cases (48%) and catenin accumulation in the nucleus in five cases (11%). Of four renal pelvis TCC examined, point mutation of exon 3 of the β-catenin gene at codon 45 resulting in amino acid substitution (Ser to Phe) was detected in one (25%). The incidence of reduced expression of β-catenin correlated significantly with the growth pattern (superficial type vs invasive type) of TCC (P < 0.05). These data indicate that: (1) aberrant β-catenin expression may be at least partly involved in urothelial carcinogenesis, but less significantly so in renal carcinogenesis, and (2) it may be associated with the progression of TCC showing invasive growth.

KW - β-catenin

KW - Renal cell carcinoma

KW - Renal pelvis

KW - Single strand conformation polymorphism

KW - Transitional cell carcinoma

KW - Urinary bladder

UR - http://www.scopus.com/inward/record.url?scp=0034508495&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034508495&partnerID=8YFLogxK

U2 - 10.1046/j.1440-1827.2000.01139.x

DO - 10.1046/j.1440-1827.2000.01139.x

M3 - Article

VL - 50

SP - 945

EP - 952

JO - Pathology International

JF - Pathology International

SN - 1320-5463

IS - 12

ER -