Aberrant IL-4 production by SOCS3-over-expressing T cells during infection with Leishmania major exacerbates disease manifestations

Mako Nakaya, Shinjiro Hamano, Miyuri Kawasumi, Hiroki Yoshida, Akihiko Yoshimura, Takashi Kobayashi

Research output: Contribution to journalArticle

10 Citations (Scopus)


Suppressor of cytokine signaling (SOCS) 3 is a major negative feedback regulator of signal transducer and activator of transcription 3-activating cytokines. Studies using T-cell-specific SOCS3-deficient mice indicate that the absence of SOCS3 in T cells results in exacerbation of disease progression after infection by Leishmania major due to skewing of the Th3 cell phenotype accompanied by hyper-production of IL-10 and transforming growth factor β (TGF-β). Here we show that transgenic mice over-expressing the SOCS3 gene in T cells (Lck-SOCS3 Tg mice) are also susceptible to infection by L. major. Forced expression of SOCS3 in T cells did not affect the production of the anti-inflammatory cytokines IL-10 and TGF-β or that of the protective Th1 type cytokine IFN-γ, which is required for parasite clearance. CD41 T cells isolated from infected-Lck-SOCS3 Tg mice produced much higher levels of IL-4 when they were restimulated with L. major antigen in vitro. Exacerbation of disease progression in Lck-SOCS3 Tg mice was completely reversed by administration of a neutralizing antibody against IL-4. These data suggest that tight regulation of SOCS3 expression in Th cells is crucial for disease control during infection by L. major.

Original languageEnglish
Pages (from-to)195-202
Number of pages8
JournalInternational immunology
Issue number3
Publication statusPublished - 2011 Mar 18



  • Cytokine
  • Regulatory T cell
  • T1
  • T2

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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