Abnormal phospholipids distribution in the prefrontal cortex from a patient with schizophrenia revealed by matrix-assisted laser desorption/ionization imaging mass spectrometry

Junya Matsumoto, Yuki Sugiura, Dai Yuki, Takahiro Hayasaka, Naoko Goto-Inoue, Nobuhiro Zaima, Yasuto Kunii, Akira Wada, Qiaohui Yang, Keisuke Nishiura, Hiroyasu Akatsu, Akira Hori, Yoshio Hashizume, Takayuki Yamamoto, Keiko Ikemoto, Mitsutoshi Setou, Shin Ichi Niwa

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Schizophrenia is one of the major psychiatric disorders, and lipids have focused on the important roles in this disorder. In fact, lipids related to various functions in the brain. Previous studies have indicated that phospholipids, particularly ones containing polyunsaturated fatty acyl residues, are deficient in postmortem brains from patients with schizophrenia. However, due to the difficulties in handling human postmortem brains, particularly the large size and complex structures of the human brain, there is little agreement regarding the qualitative and quantitative abnormalities of phospholipids in brains from patients with schizophrenia, particularly if corresponding brain regions are not used. In this study, to overcome these problems, we employed matrix-assisted laser desorption/ionization imaging mass spectrometry (IMS), enabling direct microregion analysis of phospholipids in the postmortem brain of a patient with schizophrenia via brain sections prepared on glass slides. With integration of traditional histochemical examination, we could analyze regions of interest in the brain at the micrometric level. We found abnormal phospholipid distributions within internal brain structures, namely, the frontal cortex and occipital cortex. IMS revealed abnormal distributions of phosphatidylcholine molecular species particularly in the cortical layer of frontal cortex region. In addition, the combined use of liquid chromatography/electrospray ionization tandem mass spectrometry strengthened the capability for identification of numerous lipid molecular species. Our results are expected to further elucidate various metabolic processes in the neural system.

Original languageEnglish
Pages (from-to)1933-1943
Number of pages11
JournalAnalytical and Bioanalytical Chemistry
Volume400
Issue number7
DOIs
Publication statusPublished - 2011 Jun
Externally publishedYes

Fingerprint

Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Prefrontal Cortex
Ionization
Mass spectrometry
Desorption
Brain
Phospholipids
Schizophrenia
Imaging techniques
Lasers
Frontal Lobe
Lipids
Occipital Lobe
Electrospray ionization
Electrospray Ionization Mass Spectrometry
Liquid chromatography
Tandem Mass Spectrometry
Phosphatidylcholines
Liquid Chromatography
Glass

Keywords

  • Imaging mass spectrometry (IMS)
  • Matrix-assisted laser desorption/ionization (MALDI)
  • Phospholipids
  • Postmortem brain
  • Schizophrenia

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Cite this

Abnormal phospholipids distribution in the prefrontal cortex from a patient with schizophrenia revealed by matrix-assisted laser desorption/ionization imaging mass spectrometry. / Matsumoto, Junya; Sugiura, Yuki; Yuki, Dai; Hayasaka, Takahiro; Goto-Inoue, Naoko; Zaima, Nobuhiro; Kunii, Yasuto; Wada, Akira; Yang, Qiaohui; Nishiura, Keisuke; Akatsu, Hiroyasu; Hori, Akira; Hashizume, Yoshio; Yamamoto, Takayuki; Ikemoto, Keiko; Setou, Mitsutoshi; Niwa, Shin Ichi.

In: Analytical and Bioanalytical Chemistry, Vol. 400, No. 7, 06.2011, p. 1933-1943.

Research output: Contribution to journalArticle

Matsumoto, J, Sugiura, Y, Yuki, D, Hayasaka, T, Goto-Inoue, N, Zaima, N, Kunii, Y, Wada, A, Yang, Q, Nishiura, K, Akatsu, H, Hori, A, Hashizume, Y, Yamamoto, T, Ikemoto, K, Setou, M & Niwa, SI 2011, 'Abnormal phospholipids distribution in the prefrontal cortex from a patient with schizophrenia revealed by matrix-assisted laser desorption/ionization imaging mass spectrometry', Analytical and Bioanalytical Chemistry, vol. 400, no. 7, pp. 1933-1943. https://doi.org/10.1007/s00216-011-4909-3
Matsumoto, Junya ; Sugiura, Yuki ; Yuki, Dai ; Hayasaka, Takahiro ; Goto-Inoue, Naoko ; Zaima, Nobuhiro ; Kunii, Yasuto ; Wada, Akira ; Yang, Qiaohui ; Nishiura, Keisuke ; Akatsu, Hiroyasu ; Hori, Akira ; Hashizume, Yoshio ; Yamamoto, Takayuki ; Ikemoto, Keiko ; Setou, Mitsutoshi ; Niwa, Shin Ichi. / Abnormal phospholipids distribution in the prefrontal cortex from a patient with schizophrenia revealed by matrix-assisted laser desorption/ionization imaging mass spectrometry. In: Analytical and Bioanalytical Chemistry. 2011 ; Vol. 400, No. 7. pp. 1933-1943.
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