Abnormal protein profiles in tears with dry eye syndrome

Yoshiki Ohashi, Reiko Ishida, Takashi Kojima, Eiki Goto, Yukihiro Matsumoto, Katsuhiko Watanabe, Naruhiro Ishida, Katsuhiko Nakata, Tsutomu Takeuchi, Kazuo Tsubota

Research output: Contribution to journalArticle

129 Citations (Scopus)

Abstract

PURPOSE: To verify the hypothesis that protein concentrations, such as lactoferrin, epidermal growth factor (EGF), and aquaporin 5 (AQP5), in tears are abnormal in patients with dry eye. DESIGN: Prospective case-control study. METHODS: One hundred three dry eye patients were divided into three groups: dry eye not associated with the Sjögren syndrome (non-SS; n = 71), Sjögren syndrome (SS; n = 23), and Stevens-Johnson syndrome (SJS; n = 9). Sixteen normal control subjects were also checked. The concentrations of lactoerrin, EGF, and AQP5 were measured by enzyme-linked immunosorbent assay. RESULTS: The concentration of lactoferrin was significantly decreased in tears of non-SS (P = .0001), SS (P = .00005), and SJS (P = .0006) patients compared with control subjects. The concentration of EGF was significantly decreased in non-SS (P = .0005), SS (P = .00002), and SJS (P = .0001) patients compared with control subjects. The concentration of AQP5 was significantly increased in tears of only SS patients (P = .01) compared with control subjects and increased in tears of only SS patients compared with non-SS patients (P = .007). CONCLUSIONS: The decrease in both lactoferrin and EGF was found not only in SS patients but also in non-SS patients, indicating that tear components in dry eyes differ in their quantity and quality. Quantification of AQP5 increased only in SS patients, suggesting that AQP5 protein leaks into the tears when acinar cells of the lacrimal gland are damaged by lymphocytic infiltration.

Original languageEnglish
Pages (from-to)291-299
Number of pages9
JournalAmerican Journal of Ophthalmology
Volume136
Issue number2
DOIs
Publication statusPublished - 2003 Aug 1

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Dry Eye Syndromes
Tears
Aquaporin 5
Proteins
Epidermal Growth Factor
Lactoferrin
Lacrimal Apparatus
Stevens-Johnson Syndrome
Acinar Cells
Case-Control Studies
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Ohashi, Y., Ishida, R., Kojima, T., Goto, E., Matsumoto, Y., Watanabe, K., ... Tsubota, K. (2003). Abnormal protein profiles in tears with dry eye syndrome. American Journal of Ophthalmology, 136(2), 291-299. https://doi.org/10.1016/S0002-9394(03)00203-4

Abnormal protein profiles in tears with dry eye syndrome. / Ohashi, Yoshiki; Ishida, Reiko; Kojima, Takashi; Goto, Eiki; Matsumoto, Yukihiro; Watanabe, Katsuhiko; Ishida, Naruhiro; Nakata, Katsuhiko; Takeuchi, Tsutomu; Tsubota, Kazuo.

In: American Journal of Ophthalmology, Vol. 136, No. 2, 01.08.2003, p. 291-299.

Research output: Contribution to journalArticle

Ohashi, Y, Ishida, R, Kojima, T, Goto, E, Matsumoto, Y, Watanabe, K, Ishida, N, Nakata, K, Takeuchi, T & Tsubota, K 2003, 'Abnormal protein profiles in tears with dry eye syndrome', American Journal of Ophthalmology, vol. 136, no. 2, pp. 291-299. https://doi.org/10.1016/S0002-9394(03)00203-4
Ohashi Y, Ishida R, Kojima T, Goto E, Matsumoto Y, Watanabe K et al. Abnormal protein profiles in tears with dry eye syndrome. American Journal of Ophthalmology. 2003 Aug 1;136(2):291-299. https://doi.org/10.1016/S0002-9394(03)00203-4
Ohashi, Yoshiki ; Ishida, Reiko ; Kojima, Takashi ; Goto, Eiki ; Matsumoto, Yukihiro ; Watanabe, Katsuhiko ; Ishida, Naruhiro ; Nakata, Katsuhiko ; Takeuchi, Tsutomu ; Tsubota, Kazuo. / Abnormal protein profiles in tears with dry eye syndrome. In: American Journal of Ophthalmology. 2003 ; Vol. 136, No. 2. pp. 291-299.
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AU - Ohashi, Yoshiki

AU - Ishida, Reiko

AU - Kojima, Takashi

AU - Goto, Eiki

AU - Matsumoto, Yukihiro

AU - Watanabe, Katsuhiko

AU - Ishida, Naruhiro

AU - Nakata, Katsuhiko

AU - Takeuchi, Tsutomu

AU - Tsubota, Kazuo

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N2 - PURPOSE: To verify the hypothesis that protein concentrations, such as lactoferrin, epidermal growth factor (EGF), and aquaporin 5 (AQP5), in tears are abnormal in patients with dry eye. DESIGN: Prospective case-control study. METHODS: One hundred three dry eye patients were divided into three groups: dry eye not associated with the Sjögren syndrome (non-SS; n = 71), Sjögren syndrome (SS; n = 23), and Stevens-Johnson syndrome (SJS; n = 9). Sixteen normal control subjects were also checked. The concentrations of lactoerrin, EGF, and AQP5 were measured by enzyme-linked immunosorbent assay. RESULTS: The concentration of lactoferrin was significantly decreased in tears of non-SS (P = .0001), SS (P = .00005), and SJS (P = .0006) patients compared with control subjects. The concentration of EGF was significantly decreased in non-SS (P = .0005), SS (P = .00002), and SJS (P = .0001) patients compared with control subjects. The concentration of AQP5 was significantly increased in tears of only SS patients (P = .01) compared with control subjects and increased in tears of only SS patients compared with non-SS patients (P = .007). CONCLUSIONS: The decrease in both lactoferrin and EGF was found not only in SS patients but also in non-SS patients, indicating that tear components in dry eyes differ in their quantity and quality. Quantification of AQP5 increased only in SS patients, suggesting that AQP5 protein leaks into the tears when acinar cells of the lacrimal gland are damaged by lymphocytic infiltration.

AB - PURPOSE: To verify the hypothesis that protein concentrations, such as lactoferrin, epidermal growth factor (EGF), and aquaporin 5 (AQP5), in tears are abnormal in patients with dry eye. DESIGN: Prospective case-control study. METHODS: One hundred three dry eye patients were divided into three groups: dry eye not associated with the Sjögren syndrome (non-SS; n = 71), Sjögren syndrome (SS; n = 23), and Stevens-Johnson syndrome (SJS; n = 9). Sixteen normal control subjects were also checked. The concentrations of lactoerrin, EGF, and AQP5 were measured by enzyme-linked immunosorbent assay. RESULTS: The concentration of lactoferrin was significantly decreased in tears of non-SS (P = .0001), SS (P = .00005), and SJS (P = .0006) patients compared with control subjects. The concentration of EGF was significantly decreased in non-SS (P = .0005), SS (P = .00002), and SJS (P = .0001) patients compared with control subjects. The concentration of AQP5 was significantly increased in tears of only SS patients (P = .01) compared with control subjects and increased in tears of only SS patients compared with non-SS patients (P = .007). CONCLUSIONS: The decrease in both lactoferrin and EGF was found not only in SS patients but also in non-SS patients, indicating that tear components in dry eyes differ in their quantity and quality. Quantification of AQP5 increased only in SS patients, suggesting that AQP5 protein leaks into the tears when acinar cells of the lacrimal gland are damaged by lymphocytic infiltration.

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