Accelerated adhesion of grafted skin by laser-induced stress wave-based gene transfer of hepatocyte growth factor

Kazuya Aizawa, Shunichi Sato, Mitsuhiro Terakawa, Daizoh Saitoh, Hitoshi Tsuda, Hiroshi Ashida, Minoru Obara

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Gene therapy using wound healing-associated growth factor gene has received much attention as a new strategy for improving the outcome of tissue transplantation. We delivered plasmid DNA coding for human hepatocyte growth factor (hHGF) to rat free skin grafts by the use of laser-induced stress waves (LISWs); autografting was performed with the grafts. Systematic analysis was conducted to evaluate the adhesion properties of the grafted tissue; angiogenesis, cell proliferation, and reepithelialization were assessed by immunohistochemistry, and reperfusion was measured by laser Doppler imaging as a function of time after grafting. Both the level of angiogenesis on day 3 after grafting and the increased ratio of blood flow on day 4 to that on day 3 were significantly higher than those in five control groups: grafting with hHGF gene injection alone, grafting with control plasmid vector injection alone, grafting with LISW application alone, grafting with LISW application after control plasmid vector injection, and normal grafting. Reepithelialization was almost completed on day 7 even at the center of the graft with LISW application after hHGF gene injection, while it was not for the grafts of the five control groups. These findings demonstrate the validity of our LISW-based HGF gene transfection to accelerate the adhesion of grafted skins.

Original languageEnglish
Article number064043
JournalJournal of Biomedical Optics
Volume14
Issue number6
DOIs
Publication statusPublished - 2009

Fingerprint

Gene transfer
stress waves
Hepatocyte Growth Factor
genes
Skin
adhesion
Adhesion
Lasers
Grafts
plasmids
lasers
Genes
injection
angiogenesis
Plasmids
skin grafts
Transplantation (surgical)
gene therapy
wound healing
Gene therapy

Keywords

  • Hepatocyte growth factor (HGF)
  • Laser-induced stress wave (LISW)
  • Nonviral gene delivery
  • Skin grafting

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

Accelerated adhesion of grafted skin by laser-induced stress wave-based gene transfer of hepatocyte growth factor. / Aizawa, Kazuya; Sato, Shunichi; Terakawa, Mitsuhiro; Saitoh, Daizoh; Tsuda, Hitoshi; Ashida, Hiroshi; Obara, Minoru.

In: Journal of Biomedical Optics, Vol. 14, No. 6, 064043, 2009.

Research output: Contribution to journalArticle

Aizawa, Kazuya ; Sato, Shunichi ; Terakawa, Mitsuhiro ; Saitoh, Daizoh ; Tsuda, Hitoshi ; Ashida, Hiroshi ; Obara, Minoru. / Accelerated adhesion of grafted skin by laser-induced stress wave-based gene transfer of hepatocyte growth factor. In: Journal of Biomedical Optics. 2009 ; Vol. 14, No. 6.
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