Acceleration of osteogenesis via stimulation of angiogenesis by combination with scaffold and connective tissue growth factor

Michiyo Honda, Ryo Hariya, Morio Matsumoto, Mamoru Aizawa

Research output: Contribution to journalArticle

Abstract

In bone regeneration, there are some important cellular biological processes, such as mineralization, cell organization, and differentiation. In particular, vascularization into regenerative tissues is a key step for the survival of cells and tissues. In this study, to fabricate biomimetic-engineered bone, including vascular networks, we focused on connective tissue growth factor (CTGF), a multifunctional protein which could regulate the extracellular matrix remodeling. By combination with CTGF and hydroxyapatite (HAp) ceramics (2D) or apatite-fiber scaffold (AFS, 3D), we have fabricated bioactive materials. The CTGF-loaded HAp ceramics could enhance the cellular attachment through interaction with integrin and promote actin cytoskeletal reorganization. CTGF-loaded HAp also enhanced the differentiation of osteoblasts by integrin-mediated activation of the signaling pathway. Under co-culture conditions, both osteoblasts and endothelial cells in the CTGF-loaded AFS were stimulated by CTGF, and each cell could penetrate the central region of the scaffold in vitro and in vivo. Direct cell-cell interaction would also improve the functionality of cells in bone formation. These results suggest that coupling between effective optimized scaffold and CTGF with multifunction could provide better mimicking natural bone by stimulation of angiogenesis.

Original languageEnglish
Article number2068
JournalMaterials
Volume12
Issue number13
DOIs
Publication statusPublished - 2019 Jul 1

Fingerprint

Connective Tissue Growth Factor
Scaffolds
Tissue
Bone
Durapatite
Hydroxyapatite
Osteoblasts
Integrins
Apatites
Endothelial cells
Biomimetics
Intercellular Signaling Peptides and Proteins
Cell culture
Actins
Apatite
Chemical activation
Cells
Fibers
Proteins

Keywords

  • CTGF
  • Hydroxyapatite
  • Osteogenesis
  • Scaffold
  • Vascularization

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Acceleration of osteogenesis via stimulation of angiogenesis by combination with scaffold and connective tissue growth factor. / Honda, Michiyo; Hariya, Ryo; Matsumoto, Morio; Aizawa, Mamoru.

In: Materials, Vol. 12, No. 13, 2068, 01.07.2019.

Research output: Contribution to journalArticle

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