Accumulated photon-echo spectroscopy for stained tissue samples

K. Uchikawa, Michiie Sakamoto, A. Koreeda, S. Saikan

Research output: Contribution to journalArticle

Abstract

We performed phase relaxation-time measurements of the accumulated photon echo for normal and cancerous tissue samples of human liver, that were stained with several kinds of dye. Each dye interacted selectively with a specific kind of biomolecule in the tissues, for example, DNA, proteins, or lipids. The cancerous tissues showed a tendency to have shorter relaxation times than the normal tissues had. The difference of relaxation time observed between the normal and the cancerous tissues depended on the dyes that were used for staining, and the difference became most clear when the tissues were stained with a cyanine dye derivative, i.e., YOPRo3 Iodide, which has a high selectivity for DNA. We performed the relaxation-time mapping of the accumulated photon echo (photon-echo imaging) for a liver tissue sample stained by YO-PR03 Iodide. The photonecho imaging was successful in showing a region of a highly differentiated hepatocellular carcinoma in the tissue sample, while fluorescence-intensity mapping could not identify the cancerous region in the same tissue sample. These results indicated a possibility that the reduction of accumulated photon-echo relaxation time observed in the cancerous tissues was caused by a change of microscopic dynamics of DNA.

Original languageEnglish
Pages (from-to)1058-1067
Number of pages10
JournalJournal of the Optical Society of America B: Optical Physics
Volume17
Issue number6
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

echoes
photons
spectroscopy
relaxation time
dyes
deoxyribonucleic acid
liver
iodides
staining
lipids
tendencies
selectivity
cancer
time measurement
proteins
fluorescence

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Accumulated photon-echo spectroscopy for stained tissue samples. / Uchikawa, K.; Sakamoto, Michiie; Koreeda, A.; Saikan, S.

In: Journal of the Optical Society of America B: Optical Physics, Vol. 17, No. 6, 2000, p. 1058-1067.

Research output: Contribution to journalArticle

@article{f85c5d830aef42ba9cfd8a100981bb6b,
title = "Accumulated photon-echo spectroscopy for stained tissue samples",
abstract = "We performed phase relaxation-time measurements of the accumulated photon echo for normal and cancerous tissue samples of human liver, that were stained with several kinds of dye. Each dye interacted selectively with a specific kind of biomolecule in the tissues, for example, DNA, proteins, or lipids. The cancerous tissues showed a tendency to have shorter relaxation times than the normal tissues had. The difference of relaxation time observed between the normal and the cancerous tissues depended on the dyes that were used for staining, and the difference became most clear when the tissues were stained with a cyanine dye derivative, i.e., YOPRo3 Iodide, which has a high selectivity for DNA. We performed the relaxation-time mapping of the accumulated photon echo (photon-echo imaging) for a liver tissue sample stained by YO-PR03 Iodide. The photonecho imaging was successful in showing a region of a highly differentiated hepatocellular carcinoma in the tissue sample, while fluorescence-intensity mapping could not identify the cancerous region in the same tissue sample. These results indicated a possibility that the reduction of accumulated photon-echo relaxation time observed in the cancerous tissues was caused by a change of microscopic dynamics of DNA.",
author = "K. Uchikawa and Michiie Sakamoto and A. Koreeda and S. Saikan",
year = "2000",
language = "English",
volume = "17",
pages = "1058--1067",
journal = "Journal of the Optical Society of America B: Optical Physics",
issn = "0740-3224",
publisher = "The Optical Society",
number = "6",

}

TY - JOUR

T1 - Accumulated photon-echo spectroscopy for stained tissue samples

AU - Uchikawa, K.

AU - Sakamoto, Michiie

AU - Koreeda, A.

AU - Saikan, S.

PY - 2000

Y1 - 2000

N2 - We performed phase relaxation-time measurements of the accumulated photon echo for normal and cancerous tissue samples of human liver, that were stained with several kinds of dye. Each dye interacted selectively with a specific kind of biomolecule in the tissues, for example, DNA, proteins, or lipids. The cancerous tissues showed a tendency to have shorter relaxation times than the normal tissues had. The difference of relaxation time observed between the normal and the cancerous tissues depended on the dyes that were used for staining, and the difference became most clear when the tissues were stained with a cyanine dye derivative, i.e., YOPRo3 Iodide, which has a high selectivity for DNA. We performed the relaxation-time mapping of the accumulated photon echo (photon-echo imaging) for a liver tissue sample stained by YO-PR03 Iodide. The photonecho imaging was successful in showing a region of a highly differentiated hepatocellular carcinoma in the tissue sample, while fluorescence-intensity mapping could not identify the cancerous region in the same tissue sample. These results indicated a possibility that the reduction of accumulated photon-echo relaxation time observed in the cancerous tissues was caused by a change of microscopic dynamics of DNA.

AB - We performed phase relaxation-time measurements of the accumulated photon echo for normal and cancerous tissue samples of human liver, that were stained with several kinds of dye. Each dye interacted selectively with a specific kind of biomolecule in the tissues, for example, DNA, proteins, or lipids. The cancerous tissues showed a tendency to have shorter relaxation times than the normal tissues had. The difference of relaxation time observed between the normal and the cancerous tissues depended on the dyes that were used for staining, and the difference became most clear when the tissues were stained with a cyanine dye derivative, i.e., YOPRo3 Iodide, which has a high selectivity for DNA. We performed the relaxation-time mapping of the accumulated photon echo (photon-echo imaging) for a liver tissue sample stained by YO-PR03 Iodide. The photonecho imaging was successful in showing a region of a highly differentiated hepatocellular carcinoma in the tissue sample, while fluorescence-intensity mapping could not identify the cancerous region in the same tissue sample. These results indicated a possibility that the reduction of accumulated photon-echo relaxation time observed in the cancerous tissues was caused by a change of microscopic dynamics of DNA.

UR - http://www.scopus.com/inward/record.url?scp=0034391378&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034391378&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0034391378

VL - 17

SP - 1058

EP - 1067

JO - Journal of the Optical Society of America B: Optical Physics

JF - Journal of the Optical Society of America B: Optical Physics

SN - 0740-3224

IS - 6

ER -