Accumulation of AMPA Receptors in Autophagosomes in Neuronal Axons Lacking Adaptor Protein AP-4

Shinji Matsuda, Eriko Miura, Keiko Matsuda, Wataru Kakegawa, Kazuhisa Kohda, Masahiko Watanabe, Michisuke Yuzaki

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

AP-4 is a member of the adaptor protein complexes, which control vesicular trafficking of membrane proteins. Although AP-4 has been suggested to contribute to basolateral sorting in epithelial cells, its function in neurons is unknown. Here, we show that disruption of the gene encoding the β subunit of AP-4 resulted in increased accumulation of axonal autophagosomes, which contained AMPA receptors and transmembrane AMPA receptor regulatory proteins (TARPs), in axons of hippocampal neurons and cerebellar Purkinje cells both in vitro and in vivo. AP-4 indirectly associated with the AMPA receptor via TARPs, and the specific disruption of the interaction between AP-4 and TARPs caused the mislocalization of endogenous AMPA receptors in axons of wild-type neurons. These results indicate that AP-4 may regulate proper somatodendritic-specific distribution of its cargo proteins, including AMPA receptor-TARP complexes and the autophagic pathway in neurons.

Original languageEnglish
Pages (from-to)730-745
Number of pages16
JournalNeuron
Volume57
Issue number5
DOIs
Publication statusPublished - 2008 Mar 13

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AMPA Receptors
Axons
Proteins
Neurons
Autophagosomes
Purkinje Cells
Membrane Proteins
Epithelial Cells

Keywords

  • CELLBIO
  • MOLNEURO
  • PROTEINS

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Accumulation of AMPA Receptors in Autophagosomes in Neuronal Axons Lacking Adaptor Protein AP-4. / Matsuda, Shinji; Miura, Eriko; Matsuda, Keiko; Kakegawa, Wataru; Kohda, Kazuhisa; Watanabe, Masahiko; Yuzaki, Michisuke.

In: Neuron, Vol. 57, No. 5, 13.03.2008, p. 730-745.

Research output: Contribution to journalArticle

Matsuda, Shinji ; Miura, Eriko ; Matsuda, Keiko ; Kakegawa, Wataru ; Kohda, Kazuhisa ; Watanabe, Masahiko ; Yuzaki, Michisuke. / Accumulation of AMPA Receptors in Autophagosomes in Neuronal Axons Lacking Adaptor Protein AP-4. In: Neuron. 2008 ; Vol. 57, No. 5. pp. 730-745.
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