Accumulation of Secretory Vesicles in the Lacrimal Gland Epithelia Is Related to Non-Sjögren's Type Dry Eye in Visual Display Terminal Users

Mizuka Kamoi, Yoko Ogawa, Shigeru Nakamura, Murat Dogru, Toshihiro Nagai, Hiroto Obata, Masataka Ito, Minako Kaido, Tetsuya Kawakita, Yasunori Okada, Yutaka Kawakami, Shigeto Shimmura, Kazuo Tsubota

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Previous observations in a rat model of a non-Sjögren's syndrome (non-SS) type of dry eye seen in users of visual display terminals (VDT) indicated that secretory vesicle (SV) accumulation in the lacrimal gland epithelia contributes to the condition. Here, to examine this possibility in humans, we compared the lacrimal gland histology and percent SV area in the cytoplasm of acinar epithelial cells using light microscopy and transmission electron microscopy, in patients with VDT work-related non-SS dry-eye (VDT group), SS-induced dry-eye, and autopsied normal controls. In addition, the VAMP8 (vesicle-associated membrane protein 8, an exocrine-pathway molecule) and Rab3D (mature vesicle marker) were histochemically examined in lacrimal gland tissue sections. The lacrimal gland acini were larger in the VDT group than in the SS group, and the percent SV area was significantly higher in the VDT group than in the normal controls (P = 0.021) or SS group (P = 0.004). Immunostaining revealed abnormal distributions of VAMP8 in the VDT and SS groups. Rab3D was more strongly expressed in the cytoplasm of acinar epithelial cells in the VDT group than in that of normal controls. The duration of VDT use was significantly longer in the VDT group than in the other groups. These findings suggest that excessive SV accumulation in the acinar epithelia may contribute to the reduced tear secretion in VDT users.

Original languageEnglish
Article numbere43688
JournalPLoS One
Volume7
Issue number9
DOIs
Publication statusPublished - 2012 Sep 4

Fingerprint

lacrimal apparatus
Lacrimal Apparatus
secretory granules
Secretory Vesicles
epithelium
Epithelium
eyes
Display devices
Dry Eye Syndromes
acinar cells
Acinar Cells
Cytoplasm
epithelial cells
cytoplasm
Epithelial Cells
R-SNARE Proteins
Transmission Electron Microscopy
Tears
membrane proteins
histology

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Accumulation of Secretory Vesicles in the Lacrimal Gland Epithelia Is Related to Non-Sjögren's Type Dry Eye in Visual Display Terminal Users. / Kamoi, Mizuka; Ogawa, Yoko; Nakamura, Shigeru; Dogru, Murat; Nagai, Toshihiro; Obata, Hiroto; Ito, Masataka; Kaido, Minako; Kawakita, Tetsuya; Okada, Yasunori; Kawakami, Yutaka; Shimmura, Shigeto; Tsubota, Kazuo.

In: PLoS One, Vol. 7, No. 9, e43688, 04.09.2012.

Research output: Contribution to journalArticle

Kamoi, M, Ogawa, Y, Nakamura, S, Dogru, M, Nagai, T, Obata, H, Ito, M, Kaido, M, Kawakita, T, Okada, Y, Kawakami, Y, Shimmura, S & Tsubota, K 2012, 'Accumulation of Secretory Vesicles in the Lacrimal Gland Epithelia Is Related to Non-Sjögren's Type Dry Eye in Visual Display Terminal Users', PLoS One, vol. 7, no. 9, e43688. https://doi.org/10.1371/journal.pone.0043688
Kamoi, Mizuka ; Ogawa, Yoko ; Nakamura, Shigeru ; Dogru, Murat ; Nagai, Toshihiro ; Obata, Hiroto ; Ito, Masataka ; Kaido, Minako ; Kawakita, Tetsuya ; Okada, Yasunori ; Kawakami, Yutaka ; Shimmura, Shigeto ; Tsubota, Kazuo. / Accumulation of Secretory Vesicles in the Lacrimal Gland Epithelia Is Related to Non-Sjögren's Type Dry Eye in Visual Display Terminal Users. In: PLoS One. 2012 ; Vol. 7, No. 9.
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