Accurate multiplex polymerase chain reaction assay for gender determination from a single cell

T. Hashiba, Kou Sueoka, M. Kuroshima, H. Asada, N. Kuji, Y. Yoshimura

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

For gender determination of preimplantation embryos or circulating fetal cells in maternal blood, we developed a multiplex polymerase chain reaction assay from a single cell. This assay which co-amplifies X (DXZ1)- and Y (DYZ1)-specific repeat sequences, yields a 308-bp band in females and two bands of 154 and 308 bp in males. In a randomized, blinded assay of 100 isolated single amniocytes, 99 (99%) were amplified successfully. All 50 of the XY cells were correctly diagnosed as male (100%), whereas 49 of the 50 XX cells were diagnosed as female (98%). This accurate and efficient assay may be applicable in these clinical settings. Copyright (C) 2000 S. Karger AG, Basel.

Original languageEnglish
Pages (from-to)217-220
Number of pages4
JournalGynecologic and Obstetric Investigation
Volume49
Issue number4
Publication statusPublished - 2000

Fingerprint

Multiplex Polymerase Chain Reaction
Blastocyst
Mothers

Keywords

  • DXZ1
  • DYZ1
  • Gender determination
  • Multiplex polymerase chain reaction
  • Preimplantation genetic diagnosis

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

Accurate multiplex polymerase chain reaction assay for gender determination from a single cell. / Hashiba, T.; Sueoka, Kou; Kuroshima, M.; Asada, H.; Kuji, N.; Yoshimura, Y.

In: Gynecologic and Obstetric Investigation, Vol. 49, No. 4, 2000, p. 217-220.

Research output: Contribution to journalArticle

Hashiba, T, Sueoka, K, Kuroshima, M, Asada, H, Kuji, N & Yoshimura, Y 2000, 'Accurate multiplex polymerase chain reaction assay for gender determination from a single cell', Gynecologic and Obstetric Investigation, vol. 49, no. 4, pp. 217-220.
Hashiba, T. ; Sueoka, Kou ; Kuroshima, M. ; Asada, H. ; Kuji, N. ; Yoshimura, Y. / Accurate multiplex polymerase chain reaction assay for gender determination from a single cell. In: Gynecologic and Obstetric Investigation. 2000 ; Vol. 49, No. 4. pp. 217-220.
@article{a590de61e11a465da6ff037939f8a72b,
title = "Accurate multiplex polymerase chain reaction assay for gender determination from a single cell",
abstract = "For gender determination of preimplantation embryos or circulating fetal cells in maternal blood, we developed a multiplex polymerase chain reaction assay from a single cell. This assay which co-amplifies X (DXZ1)- and Y (DYZ1)-specific repeat sequences, yields a 308-bp band in females and two bands of 154 and 308 bp in males. In a randomized, blinded assay of 100 isolated single amniocytes, 99 (99{\%}) were amplified successfully. All 50 of the XY cells were correctly diagnosed as male (100{\%}), whereas 49 of the 50 XX cells were diagnosed as female (98{\%}). This accurate and efficient assay may be applicable in these clinical settings. Copyright (C) 2000 S. Karger AG, Basel.",
keywords = "DXZ1, DYZ1, Gender determination, Multiplex polymerase chain reaction, Preimplantation genetic diagnosis",
author = "T. Hashiba and Kou Sueoka and M. Kuroshima and H. Asada and N. Kuji and Y. Yoshimura",
year = "2000",
language = "English",
volume = "49",
pages = "217--220",
journal = "Gynecologic and Obstetric Investigation",
issn = "0378-7346",
publisher = "S. Karger AG",
number = "4",

}

TY - JOUR

T1 - Accurate multiplex polymerase chain reaction assay for gender determination from a single cell

AU - Hashiba, T.

AU - Sueoka, Kou

AU - Kuroshima, M.

AU - Asada, H.

AU - Kuji, N.

AU - Yoshimura, Y.

PY - 2000

Y1 - 2000

N2 - For gender determination of preimplantation embryos or circulating fetal cells in maternal blood, we developed a multiplex polymerase chain reaction assay from a single cell. This assay which co-amplifies X (DXZ1)- and Y (DYZ1)-specific repeat sequences, yields a 308-bp band in females and two bands of 154 and 308 bp in males. In a randomized, blinded assay of 100 isolated single amniocytes, 99 (99%) were amplified successfully. All 50 of the XY cells were correctly diagnosed as male (100%), whereas 49 of the 50 XX cells were diagnosed as female (98%). This accurate and efficient assay may be applicable in these clinical settings. Copyright (C) 2000 S. Karger AG, Basel.

AB - For gender determination of preimplantation embryos or circulating fetal cells in maternal blood, we developed a multiplex polymerase chain reaction assay from a single cell. This assay which co-amplifies X (DXZ1)- and Y (DYZ1)-specific repeat sequences, yields a 308-bp band in females and two bands of 154 and 308 bp in males. In a randomized, blinded assay of 100 isolated single amniocytes, 99 (99%) were amplified successfully. All 50 of the XY cells were correctly diagnosed as male (100%), whereas 49 of the 50 XX cells were diagnosed as female (98%). This accurate and efficient assay may be applicable in these clinical settings. Copyright (C) 2000 S. Karger AG, Basel.

KW - DXZ1

KW - DYZ1

KW - Gender determination

KW - Multiplex polymerase chain reaction

KW - Preimplantation genetic diagnosis

UR - http://www.scopus.com/inward/record.url?scp=0034066333&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034066333&partnerID=8YFLogxK

M3 - Article

C2 - 10828701

AN - SCOPUS:0034066333

VL - 49

SP - 217

EP - 220

JO - Gynecologic and Obstetric Investigation

JF - Gynecologic and Obstetric Investigation

SN - 0378-7346

IS - 4

ER -