Accurate multiplex polymerase chain reaction assay for gender determination from a single cell

T. Hashiba, K. Sueoka, M. Kuroshima, H. Asada, N. Kuji, Y. Yoshimura

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

For gender determination of preimplantation embryos or circulating fetal cells in maternal blood, we developed a multiplex polymerase chain reaction assay from a single cell. This assay which co-amplifies X (DXZ1)- and Y (DYZ1)-specific repeat sequences, yields a 308-bp band in females and two bands of 154 and 308 bp in males. In a randomized, blinded assay of 100 isolated single amniocytes, 99 (99%) were amplified successfully. All 50 of the XY cells were correctly diagnosed as male (100%), whereas 49 of the 50 XX cells were diagnosed as female (98%). This accurate and efficient assay may be applicable in these clinical settings. Copyright (C) 2000 S. Karger AG, Basel.

Original languageEnglish
Pages (from-to)217-220
Number of pages4
JournalGynecologic and Obstetric Investigation
Volume49
Issue number4
DOIs
Publication statusPublished - 2000 Jan 1

Keywords

  • DXZ1
  • DYZ1
  • Gender determination
  • Multiplex polymerase chain reaction
  • Preimplantation genetic diagnosis

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynaecology

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