Acellularity of starfish embryonic mesenchyme cells as shown in vitro

Hiroyuki Kaneko, Shigeko Takaichi, Masamichi Yamamoto, Marina Dan-Sohkawa

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Embryonic mesenchyme cells of the starfish are shown to be unexpectedly fusogenic in vitro. When archenteron complexes (archenterons and varying portions of the extracellular matrix {ECM} surrounding them) are isolated from starfish embryos and inoculated in sea water containing 4 % newborn bovine serum, the mesenchyme cells form large syncytia on the substratum underneath each archenteron. These syncytia break into smaller fragments interconnected by fine cell processes within 24h. These networks have been studied morphologically, dynamically and ultrastructurally and found to lack cell borders between the constituent fragments. These fragments contain various numbers of nuclei ranging from 0 to 6 or more and move about constantly over the substratum, sometimes breaking into two and sometimes fusing with neighbouring fragments, so that the overall pattern of the network changes constantly. Our results also indicate that the network is three-dimensional i.e. it has crossing sites, the frequency of which seems to depend on the amount of the ECM excreted on the substratum. A similar network pattern is found among mesenchyme cells in vivo, which suggests that the features found in vitro reflect those in vivo.

Original languageEnglish
Pages (from-to)129-138
Number of pages10
JournalDevelopment
Volume109
Issue number1
Publication statusPublished - 1990 May
Externally publishedYes

Fingerprint

Starfish
Mesoderm
Gastrula
Giant Cells
Extracellular Matrix
Seawater
Embryonic Structures
In Vitro Techniques
Serum

Keywords

  • Acellularity
  • Dynamic network
  • In vitro
  • Mesenchyme cells
  • Starfish embryo
  • Three-dimensional network

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Kaneko, H., Takaichi, S., Yamamoto, M., & Dan-Sohkawa, M. (1990). Acellularity of starfish embryonic mesenchyme cells as shown in vitro. Development, 109(1), 129-138.

Acellularity of starfish embryonic mesenchyme cells as shown in vitro. / Kaneko, Hiroyuki; Takaichi, Shigeko; Yamamoto, Masamichi; Dan-Sohkawa, Marina.

In: Development, Vol. 109, No. 1, 05.1990, p. 129-138.

Research output: Contribution to journalArticle

Kaneko, H, Takaichi, S, Yamamoto, M & Dan-Sohkawa, M 1990, 'Acellularity of starfish embryonic mesenchyme cells as shown in vitro', Development, vol. 109, no. 1, pp. 129-138.
Kaneko H, Takaichi S, Yamamoto M, Dan-Sohkawa M. Acellularity of starfish embryonic mesenchyme cells as shown in vitro. Development. 1990 May;109(1):129-138.
Kaneko, Hiroyuki ; Takaichi, Shigeko ; Yamamoto, Masamichi ; Dan-Sohkawa, Marina. / Acellularity of starfish embryonic mesenchyme cells as shown in vitro. In: Development. 1990 ; Vol. 109, No. 1. pp. 129-138.
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