Acetaldehyde inhibits the formation of retinoic acid from retinal in the rat esophagus

Haruko Shiraishi-Yokoyama, Hirokazu Yokoyama, Michinaga Matsumoto, Hiroyuki Imaeda, Toshifumi Hibi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Objective. It has already been demonstrated that the rat esophagus produces retinoic acid from retinol. In this study, this process is further characterized and the effect of acetaldehyde examined to elucidate the possible mechanisms behind the epidemiological evidence that the incidence of esophageal cancer is higher in alcoholics. Material and methods. Rat esophageal samples were incubated with all-trans retinal and newly formed all-trans retinoic acid (ATRA) was quantified using high-performance liquid chromatography (HPLC). Furthermore, β-nicotinamide adenine dinucleotide (NAD)-dependent acetaldehyde oxidation by the rat esophagus was examined by tracing NAD reduction using a spectrophotometer. Results. Rat esophageal samples produced ATRA from all-trans retinal in a NAD-dependent manner and the potential was significantly attenuated by phenetyl isothiocynate, an ALDH inhibitor, or acetaldehyde depending on the concentration used. Rat esophageal samples also oxidized acetaldehyde of various concentrations NAD dependently. The ATRA formation potential that was temporarily inhibited by acetaldehyde was recovered to the control level by dialysis when the specimen was incubated with up to 50 μM of acetaldehyde. Conclusions. The rat esophagus produces retinoic acid from retinal. An ALDH isoform(s) is responsible for this process and physiological concentration of acetaldehyde hampers the process, probably in a competitive manner. Since the disturbance of retinoic acid supply has been implicated in carcinogenicity, this finding may, at least in part, explain the high incidence of esophageal cancer in alcoholics, especially in those with inactive ALDH 2 whose blood acetaldehyde levels become higher than those with active ALDH 2.

Original languageEnglish
Pages (from-to)80-86
Number of pages7
JournalScandinavian Journal of Gastroenterology
Volume41
Issue number1
DOIs
Publication statusPublished - 2006 Jan

Fingerprint

Acetaldehyde
Tretinoin
Esophagus
NAD
Alcoholics
Esophageal Neoplasms
Physiological Phenomena
Incidence
Vitamin A
Dialysis
Protein Isoforms
High Pressure Liquid Chromatography

Keywords

  • Acetaldehyde
  • Alcoholics
  • Esophageal cancer
  • Retinal
  • Retinoic acid

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Acetaldehyde inhibits the formation of retinoic acid from retinal in the rat esophagus. / Shiraishi-Yokoyama, Haruko; Yokoyama, Hirokazu; Matsumoto, Michinaga; Imaeda, Hiroyuki; Hibi, Toshifumi.

In: Scandinavian Journal of Gastroenterology, Vol. 41, No. 1, 01.2006, p. 80-86.

Research output: Contribution to journalArticle

Shiraishi-Yokoyama, Haruko ; Yokoyama, Hirokazu ; Matsumoto, Michinaga ; Imaeda, Hiroyuki ; Hibi, Toshifumi. / Acetaldehyde inhibits the formation of retinoic acid from retinal in the rat esophagus. In: Scandinavian Journal of Gastroenterology. 2006 ; Vol. 41, No. 1. pp. 80-86.
@article{40b5cbd76bf24c44b0236822ed4dd0d7,
title = "Acetaldehyde inhibits the formation of retinoic acid from retinal in the rat esophagus",
abstract = "Objective. It has already been demonstrated that the rat esophagus produces retinoic acid from retinol. In this study, this process is further characterized and the effect of acetaldehyde examined to elucidate the possible mechanisms behind the epidemiological evidence that the incidence of esophageal cancer is higher in alcoholics. Material and methods. Rat esophageal samples were incubated with all-trans retinal and newly formed all-trans retinoic acid (ATRA) was quantified using high-performance liquid chromatography (HPLC). Furthermore, β-nicotinamide adenine dinucleotide (NAD)-dependent acetaldehyde oxidation by the rat esophagus was examined by tracing NAD reduction using a spectrophotometer. Results. Rat esophageal samples produced ATRA from all-trans retinal in a NAD-dependent manner and the potential was significantly attenuated by phenetyl isothiocynate, an ALDH inhibitor, or acetaldehyde depending on the concentration used. Rat esophageal samples also oxidized acetaldehyde of various concentrations NAD dependently. The ATRA formation potential that was temporarily inhibited by acetaldehyde was recovered to the control level by dialysis when the specimen was incubated with up to 50 μM of acetaldehyde. Conclusions. The rat esophagus produces retinoic acid from retinal. An ALDH isoform(s) is responsible for this process and physiological concentration of acetaldehyde hampers the process, probably in a competitive manner. Since the disturbance of retinoic acid supply has been implicated in carcinogenicity, this finding may, at least in part, explain the high incidence of esophageal cancer in alcoholics, especially in those with inactive ALDH 2 whose blood acetaldehyde levels become higher than those with active ALDH 2.",
keywords = "Acetaldehyde, Alcoholics, Esophageal cancer, Retinal, Retinoic acid",
author = "Haruko Shiraishi-Yokoyama and Hirokazu Yokoyama and Michinaga Matsumoto and Hiroyuki Imaeda and Toshifumi Hibi",
year = "2006",
month = "1",
doi = "10.1080/00365520510023936",
language = "English",
volume = "41",
pages = "80--86",
journal = "Scandinavian Journal of Gastroenterology",
issn = "0036-5521",
publisher = "Informa Healthcare",
number = "1",

}

TY - JOUR

T1 - Acetaldehyde inhibits the formation of retinoic acid from retinal in the rat esophagus

AU - Shiraishi-Yokoyama, Haruko

AU - Yokoyama, Hirokazu

AU - Matsumoto, Michinaga

AU - Imaeda, Hiroyuki

AU - Hibi, Toshifumi

PY - 2006/1

Y1 - 2006/1

N2 - Objective. It has already been demonstrated that the rat esophagus produces retinoic acid from retinol. In this study, this process is further characterized and the effect of acetaldehyde examined to elucidate the possible mechanisms behind the epidemiological evidence that the incidence of esophageal cancer is higher in alcoholics. Material and methods. Rat esophageal samples were incubated with all-trans retinal and newly formed all-trans retinoic acid (ATRA) was quantified using high-performance liquid chromatography (HPLC). Furthermore, β-nicotinamide adenine dinucleotide (NAD)-dependent acetaldehyde oxidation by the rat esophagus was examined by tracing NAD reduction using a spectrophotometer. Results. Rat esophageal samples produced ATRA from all-trans retinal in a NAD-dependent manner and the potential was significantly attenuated by phenetyl isothiocynate, an ALDH inhibitor, or acetaldehyde depending on the concentration used. Rat esophageal samples also oxidized acetaldehyde of various concentrations NAD dependently. The ATRA formation potential that was temporarily inhibited by acetaldehyde was recovered to the control level by dialysis when the specimen was incubated with up to 50 μM of acetaldehyde. Conclusions. The rat esophagus produces retinoic acid from retinal. An ALDH isoform(s) is responsible for this process and physiological concentration of acetaldehyde hampers the process, probably in a competitive manner. Since the disturbance of retinoic acid supply has been implicated in carcinogenicity, this finding may, at least in part, explain the high incidence of esophageal cancer in alcoholics, especially in those with inactive ALDH 2 whose blood acetaldehyde levels become higher than those with active ALDH 2.

AB - Objective. It has already been demonstrated that the rat esophagus produces retinoic acid from retinol. In this study, this process is further characterized and the effect of acetaldehyde examined to elucidate the possible mechanisms behind the epidemiological evidence that the incidence of esophageal cancer is higher in alcoholics. Material and methods. Rat esophageal samples were incubated with all-trans retinal and newly formed all-trans retinoic acid (ATRA) was quantified using high-performance liquid chromatography (HPLC). Furthermore, β-nicotinamide adenine dinucleotide (NAD)-dependent acetaldehyde oxidation by the rat esophagus was examined by tracing NAD reduction using a spectrophotometer. Results. Rat esophageal samples produced ATRA from all-trans retinal in a NAD-dependent manner and the potential was significantly attenuated by phenetyl isothiocynate, an ALDH inhibitor, or acetaldehyde depending on the concentration used. Rat esophageal samples also oxidized acetaldehyde of various concentrations NAD dependently. The ATRA formation potential that was temporarily inhibited by acetaldehyde was recovered to the control level by dialysis when the specimen was incubated with up to 50 μM of acetaldehyde. Conclusions. The rat esophagus produces retinoic acid from retinal. An ALDH isoform(s) is responsible for this process and physiological concentration of acetaldehyde hampers the process, probably in a competitive manner. Since the disturbance of retinoic acid supply has been implicated in carcinogenicity, this finding may, at least in part, explain the high incidence of esophageal cancer in alcoholics, especially in those with inactive ALDH 2 whose blood acetaldehyde levels become higher than those with active ALDH 2.

KW - Acetaldehyde

KW - Alcoholics

KW - Esophageal cancer

KW - Retinal

KW - Retinoic acid

UR - http://www.scopus.com/inward/record.url?scp=29744448683&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=29744448683&partnerID=8YFLogxK

U2 - 10.1080/00365520510023936

DO - 10.1080/00365520510023936

M3 - Article

C2 - 16373280

AN - SCOPUS:29744448683

VL - 41

SP - 80

EP - 86

JO - Scandinavian Journal of Gastroenterology

JF - Scandinavian Journal of Gastroenterology

SN - 0036-5521

IS - 1

ER -