Acetylcholine synthesis and muscarinic receptor subtype mRNA expression in T-lymphocytes

K. Kawashima, T. Fujii, Y. Watanabe, Hidemi Misawa

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

We used a sensitive and specific radioimmunoassay for acetylcholine (ACh), and detected significant amounts of ACh in the blood of various mammals, including humans. About 60% of human blood ACh was localized in mononuclear leukocytes. Human leukemic T-cell lines, used as T-lymphocyte models, contained both ACh and choline acetyltransferase (ChAT) activity. Furthermore, ChAT mRNA and protein were detected in the T-cell line MOLT-3. Phytohemagglutinin, a T-cell activator, increased both synthesis and release of ACh by MOLT-3 cells. Muscarinic receptor subtype mRNA expression was confirmed in various T-cell lines. These findings indicate that ACh synthesized by ChAT in T-lymphocytes acts on the muscarinic receptors on lymphocytes in autocrine and/or paracrine pathways and suggest that ACh in blood functions as a modulator of T-cell-dependent immune responses.

Original languageEnglish
Pages (from-to)1701-1705
Number of pages5
JournalLife Sciences
Volume62
Issue number17-18
DOIs
Publication statusPublished - 1998 Mar 27

Fingerprint

T-cells
Muscarinic Receptors
Acetylcholine
T-Lymphocytes
Messenger RNA
Choline O-Acetyltransferase
Blood
Cell Line
Mononuclear Leukocytes
Mammals
Lymphocytes
Phytohemagglutinins
Modulators
Radioimmunoassay

Keywords

  • Acetylcholine
  • Choline acetyltransferase
  • Muscarinic receptor
  • Radioimmunoassay
  • T-lymphocytes

ASJC Scopus subject areas

  • Pharmacology

Cite this

Acetylcholine synthesis and muscarinic receptor subtype mRNA expression in T-lymphocytes. / Kawashima, K.; Fujii, T.; Watanabe, Y.; Misawa, Hidemi.

In: Life Sciences, Vol. 62, No. 17-18, 27.03.1998, p. 1701-1705.

Research output: Contribution to journalArticle

Kawashima, K. ; Fujii, T. ; Watanabe, Y. ; Misawa, Hidemi. / Acetylcholine synthesis and muscarinic receptor subtype mRNA expression in T-lymphocytes. In: Life Sciences. 1998 ; Vol. 62, No. 17-18. pp. 1701-1705.
@article{16945ae9792a4ea7afcdc8f4f23b48ab,
title = "Acetylcholine synthesis and muscarinic receptor subtype mRNA expression in T-lymphocytes",
abstract = "We used a sensitive and specific radioimmunoassay for acetylcholine (ACh), and detected significant amounts of ACh in the blood of various mammals, including humans. About 60{\%} of human blood ACh was localized in mononuclear leukocytes. Human leukemic T-cell lines, used as T-lymphocyte models, contained both ACh and choline acetyltransferase (ChAT) activity. Furthermore, ChAT mRNA and protein were detected in the T-cell line MOLT-3. Phytohemagglutinin, a T-cell activator, increased both synthesis and release of ACh by MOLT-3 cells. Muscarinic receptor subtype mRNA expression was confirmed in various T-cell lines. These findings indicate that ACh synthesized by ChAT in T-lymphocytes acts on the muscarinic receptors on lymphocytes in autocrine and/or paracrine pathways and suggest that ACh in blood functions as a modulator of T-cell-dependent immune responses.",
keywords = "Acetylcholine, Choline acetyltransferase, Muscarinic receptor, Radioimmunoassay, T-lymphocytes",
author = "K. Kawashima and T. Fujii and Y. Watanabe and Hidemi Misawa",
year = "1998",
month = "3",
day = "27",
doi = "10.1016/S0024-3205(98)00131-3",
language = "English",
volume = "62",
pages = "1701--1705",
journal = "Life Sciences",
issn = "0024-3205",
publisher = "Elsevier Inc.",
number = "17-18",

}

TY - JOUR

T1 - Acetylcholine synthesis and muscarinic receptor subtype mRNA expression in T-lymphocytes

AU - Kawashima, K.

AU - Fujii, T.

AU - Watanabe, Y.

AU - Misawa, Hidemi

PY - 1998/3/27

Y1 - 1998/3/27

N2 - We used a sensitive and specific radioimmunoassay for acetylcholine (ACh), and detected significant amounts of ACh in the blood of various mammals, including humans. About 60% of human blood ACh was localized in mononuclear leukocytes. Human leukemic T-cell lines, used as T-lymphocyte models, contained both ACh and choline acetyltransferase (ChAT) activity. Furthermore, ChAT mRNA and protein were detected in the T-cell line MOLT-3. Phytohemagglutinin, a T-cell activator, increased both synthesis and release of ACh by MOLT-3 cells. Muscarinic receptor subtype mRNA expression was confirmed in various T-cell lines. These findings indicate that ACh synthesized by ChAT in T-lymphocytes acts on the muscarinic receptors on lymphocytes in autocrine and/or paracrine pathways and suggest that ACh in blood functions as a modulator of T-cell-dependent immune responses.

AB - We used a sensitive and specific radioimmunoassay for acetylcholine (ACh), and detected significant amounts of ACh in the blood of various mammals, including humans. About 60% of human blood ACh was localized in mononuclear leukocytes. Human leukemic T-cell lines, used as T-lymphocyte models, contained both ACh and choline acetyltransferase (ChAT) activity. Furthermore, ChAT mRNA and protein were detected in the T-cell line MOLT-3. Phytohemagglutinin, a T-cell activator, increased both synthesis and release of ACh by MOLT-3 cells. Muscarinic receptor subtype mRNA expression was confirmed in various T-cell lines. These findings indicate that ACh synthesized by ChAT in T-lymphocytes acts on the muscarinic receptors on lymphocytes in autocrine and/or paracrine pathways and suggest that ACh in blood functions as a modulator of T-cell-dependent immune responses.

KW - Acetylcholine

KW - Choline acetyltransferase

KW - Muscarinic receptor

KW - Radioimmunoassay

KW - T-lymphocytes

UR - http://www.scopus.com/inward/record.url?scp=0032571352&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032571352&partnerID=8YFLogxK

U2 - 10.1016/S0024-3205(98)00131-3

DO - 10.1016/S0024-3205(98)00131-3

M3 - Article

VL - 62

SP - 1701

EP - 1705

JO - Life Sciences

JF - Life Sciences

SN - 0024-3205

IS - 17-18

ER -