Acetylcholine synthesis and muscarinic receptor subtype mRNA expression in T-lymphocytes

K. Kawashima, T. Fujii, Y. Watanabe, H. Misawa

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

We used a sensitive and specific radioimmunoassay for acetylcholine (ACh), and detected significant amounts of ACh in the blood of various mammals, including humans. About 60% of human blood ACh was localized in mononuclear leukocytes. Human leukemic T-cell lines, used as T-lymphocyte models, contained both ACh and choline acetyltransferase (ChAT) activity. Furthermore, ChAT mRNA and protein were detected in the T-cell line MOLT-3. Phytohemagglutinin, a T-cell activator, increased both synthesis and release of ACh by MOLT-3 cells. Muscarinic receptor subtype mRNA expression was confirmed in various T-cell lines. These findings indicate that ACh synthesized by ChAT in T-lymphocytes acts on the muscarinic receptors on lymphocytes in autocrine and/or paracrine pathways and suggest that ACh in blood functions as a modulator of T-cell-dependent immune responses.

Original languageEnglish
Pages (from-to)1701-1705
Number of pages5
JournalLife Sciences
Volume62
Issue number17-18
DOIs
Publication statusPublished - 1998 Mar 27

Keywords

  • Acetylcholine
  • Choline acetyltransferase
  • Muscarinic receptor
  • Radioimmunoassay
  • T-lymphocytes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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