Acetylcholine synthesis is modulated by acetylcholine content of cytosolic fraction but not by that of releasable fraction

Takeshi Suzuki, Yuko Kashima, Kazuko Fujimoto, Koichiro Kawashima

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Synthesis and release of acetylcholine (ACh) in the rat hippocampal slices were examined to clarify the mechanism of modulation of ACh synthesis. Treatment with 2-(4-phenylpiperidino)cyclohexanol (AH5183, 50 μM), an inhibitor of ACh transport from cytosol to synaptic vesicles, inhibited the increase in ACh content of the membrane-bound fraction which is readily releasable, but did not affect the cytosolic ACh content. Under these conditions, the total ACh content reached a plateau value. These results indicate that ACh synthesis is modulated by cytosolic ACh content but not by the vesicular fraction.

Original languageEnglish
Pages (from-to)127-129
Number of pages3
JournalNeuroscience Letters
Volume144
Issue number1-2
DOIs
Publication statusPublished - 1992 Sep 14

Keywords

  • AH5183
  • Acetylcholine release
  • Acetylcholine synthesis
  • Hippocampus

ASJC Scopus subject areas

  • Neuroscience(all)

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