Acidic electrolysed water in the disinfection of the ocular surface

Shigeto Shimmura, Koki Matsumoto, Hiromoto Yaguchi, Toshiya Okuda, Seiya Miyajima, Akira Negi, Jun Shimazaki, Kazuo Tsubot

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Acidic electrolysed water (AEW) is a colorless solution prepared by the electrolysis of sodium chloride solution, which has potent anti-microbial activity against a wide variety of bacteria, fungi and virus. In this study, the safety and efficacy of an isotonic AEW solution (iAEW) was investigated both in vitro and in vivo using denuded corneas of guinea pigs. Povidone iodine (0.01-1.0%) was used as control. Exposure of P. aeruginosa to iAEW for 5 sec caused total inhibition of bacterial growth in vitro. Comparable results were obtained with 1% povidone iodine, while more dilute solutions required longer exposure times for similar effects. Cytotoxicity of iAEW on corneal epithelial cells in vitro was significantly less compared to 1% povidone iodine (P < 0.05), while minimal damage was observed in vivo by irrigation of the cornea with either iAEW or 1% povidone iodine. Treatment of P. aeruginosa with iAEW (15 sec) significantly inhibited the onset of corneal infection in guinea pigs. iAEW may offer an effective and safe method for disinfection of the ocular surface.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalExperimental Eye Research
Volume70
Issue number1
DOIs
Publication statusPublished - 2000 Jan
Externally publishedYes

Fingerprint

Isotonic Solutions
Disinfection
Povidone-Iodine
Water
Cornea
Guinea Pigs
Electrolysis
Sodium Chloride
Fungi
Epithelial Cells
Viruses
Bacteria
Safety
Growth
Infection
In Vitro Techniques

Keywords

  • Corneal ulcer
  • Disinfection
  • Free radicals
  • Oxidative damage
  • Pseudomonas keratitis

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Shimmura, S., Matsumoto, K., Yaguchi, H., Okuda, T., Miyajima, S., Negi, A., ... Tsubot, K. (2000). Acidic electrolysed water in the disinfection of the ocular surface. Experimental Eye Research, 70(1), 1-6. https://doi.org/10.1006/exer.1999.0776

Acidic electrolysed water in the disinfection of the ocular surface. / Shimmura, Shigeto; Matsumoto, Koki; Yaguchi, Hiromoto; Okuda, Toshiya; Miyajima, Seiya; Negi, Akira; Shimazaki, Jun; Tsubot, Kazuo.

In: Experimental Eye Research, Vol. 70, No. 1, 01.2000, p. 1-6.

Research output: Contribution to journalArticle

Shimmura, S, Matsumoto, K, Yaguchi, H, Okuda, T, Miyajima, S, Negi, A, Shimazaki, J & Tsubot, K 2000, 'Acidic electrolysed water in the disinfection of the ocular surface', Experimental Eye Research, vol. 70, no. 1, pp. 1-6. https://doi.org/10.1006/exer.1999.0776
Shimmura, Shigeto ; Matsumoto, Koki ; Yaguchi, Hiromoto ; Okuda, Toshiya ; Miyajima, Seiya ; Negi, Akira ; Shimazaki, Jun ; Tsubot, Kazuo. / Acidic electrolysed water in the disinfection of the ocular surface. In: Experimental Eye Research. 2000 ; Vol. 70, No. 1. pp. 1-6.
@article{8f15dd9d339644ce86ed38e8cd78bbe8,
title = "Acidic electrolysed water in the disinfection of the ocular surface",
abstract = "Acidic electrolysed water (AEW) is a colorless solution prepared by the electrolysis of sodium chloride solution, which has potent anti-microbial activity against a wide variety of bacteria, fungi and virus. In this study, the safety and efficacy of an isotonic AEW solution (iAEW) was investigated both in vitro and in vivo using denuded corneas of guinea pigs. Povidone iodine (0.01-1.0{\%}) was used as control. Exposure of P. aeruginosa to iAEW for 5 sec caused total inhibition of bacterial growth in vitro. Comparable results were obtained with 1{\%} povidone iodine, while more dilute solutions required longer exposure times for similar effects. Cytotoxicity of iAEW on corneal epithelial cells in vitro was significantly less compared to 1{\%} povidone iodine (P < 0.05), while minimal damage was observed in vivo by irrigation of the cornea with either iAEW or 1{\%} povidone iodine. Treatment of P. aeruginosa with iAEW (15 sec) significantly inhibited the onset of corneal infection in guinea pigs. iAEW may offer an effective and safe method for disinfection of the ocular surface.",
keywords = "Corneal ulcer, Disinfection, Free radicals, Oxidative damage, Pseudomonas keratitis",
author = "Shigeto Shimmura and Koki Matsumoto and Hiromoto Yaguchi and Toshiya Okuda and Seiya Miyajima and Akira Negi and Jun Shimazaki and Kazuo Tsubot",
year = "2000",
month = "1",
doi = "10.1006/exer.1999.0776",
language = "English",
volume = "70",
pages = "1--6",
journal = "Experimental Eye Research",
issn = "0014-4835",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Acidic electrolysed water in the disinfection of the ocular surface

AU - Shimmura, Shigeto

AU - Matsumoto, Koki

AU - Yaguchi, Hiromoto

AU - Okuda, Toshiya

AU - Miyajima, Seiya

AU - Negi, Akira

AU - Shimazaki, Jun

AU - Tsubot, Kazuo

PY - 2000/1

Y1 - 2000/1

N2 - Acidic electrolysed water (AEW) is a colorless solution prepared by the electrolysis of sodium chloride solution, which has potent anti-microbial activity against a wide variety of bacteria, fungi and virus. In this study, the safety and efficacy of an isotonic AEW solution (iAEW) was investigated both in vitro and in vivo using denuded corneas of guinea pigs. Povidone iodine (0.01-1.0%) was used as control. Exposure of P. aeruginosa to iAEW for 5 sec caused total inhibition of bacterial growth in vitro. Comparable results were obtained with 1% povidone iodine, while more dilute solutions required longer exposure times for similar effects. Cytotoxicity of iAEW on corneal epithelial cells in vitro was significantly less compared to 1% povidone iodine (P < 0.05), while minimal damage was observed in vivo by irrigation of the cornea with either iAEW or 1% povidone iodine. Treatment of P. aeruginosa with iAEW (15 sec) significantly inhibited the onset of corneal infection in guinea pigs. iAEW may offer an effective and safe method for disinfection of the ocular surface.

AB - Acidic electrolysed water (AEW) is a colorless solution prepared by the electrolysis of sodium chloride solution, which has potent anti-microbial activity against a wide variety of bacteria, fungi and virus. In this study, the safety and efficacy of an isotonic AEW solution (iAEW) was investigated both in vitro and in vivo using denuded corneas of guinea pigs. Povidone iodine (0.01-1.0%) was used as control. Exposure of P. aeruginosa to iAEW for 5 sec caused total inhibition of bacterial growth in vitro. Comparable results were obtained with 1% povidone iodine, while more dilute solutions required longer exposure times for similar effects. Cytotoxicity of iAEW on corneal epithelial cells in vitro was significantly less compared to 1% povidone iodine (P < 0.05), while minimal damage was observed in vivo by irrigation of the cornea with either iAEW or 1% povidone iodine. Treatment of P. aeruginosa with iAEW (15 sec) significantly inhibited the onset of corneal infection in guinea pigs. iAEW may offer an effective and safe method for disinfection of the ocular surface.

KW - Corneal ulcer

KW - Disinfection

KW - Free radicals

KW - Oxidative damage

KW - Pseudomonas keratitis

UR - http://www.scopus.com/inward/record.url?scp=0033979062&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033979062&partnerID=8YFLogxK

U2 - 10.1006/exer.1999.0776

DO - 10.1006/exer.1999.0776

M3 - Article

VL - 70

SP - 1

EP - 6

JO - Experimental Eye Research

JF - Experimental Eye Research

SN - 0014-4835

IS - 1

ER -