Action of nitrile hydratase from Rhodococcus rhodochrous IFO 15564 on derivatives of 2,5-anhydro-D-allononitrile

Masahiro Yokoyama, Yuko Nakatsuka, Takeshi Sugai, Hiromichi Ohta

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The conversion of 2,5-anhydro-D-allononitrile derivatives by a nitrile hydratase from Rhodococcus rhodochrous IFO 15564 was studied. The activity of the enzyme was strongly effected by the steric bulkiness of the substituents at the 3-position of the substrates, and the corresponding amides were obtained in high yields from the nitriles with free hydroxyl groups at the 3- and 4-positions.

Original languageEnglish
Pages (from-to)1540-1542
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume60
Issue number9
Publication statusPublished - 1996 Sep

Fingerprint

Rhodococcus rhodochrous
Rhodococcus
nitriles
Nitriles
Amides
Hydroxyl Radical
Derivatives
Substrates
Enzymes
amides
enzyme activity
nitrile hydratase

Keywords

  • 2,5-anhydro-D- allononitrile
  • 2,5-anhydro-D-allonamide
  • hydantocidin
  • nitrile hydratase
  • Rhodococcus rhodochrous IFO 15564

ASJC Scopus subject areas

  • Food Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology

Cite this

Action of nitrile hydratase from Rhodococcus rhodochrous IFO 15564 on derivatives of 2,5-anhydro-D-allononitrile. / Yokoyama, Masahiro; Nakatsuka, Yuko; Sugai, Takeshi; Ohta, Hiromichi.

In: Bioscience, Biotechnology and Biochemistry, Vol. 60, No. 9, 09.1996, p. 1540-1542.

Research output: Contribution to journalArticle

@article{1e9036adf8084849a1ad3d7f1cb65f3e,
title = "Action of nitrile hydratase from Rhodococcus rhodochrous IFO 15564 on derivatives of 2,5-anhydro-D-allononitrile",
abstract = "The conversion of 2,5-anhydro-D-allononitrile derivatives by a nitrile hydratase from Rhodococcus rhodochrous IFO 15564 was studied. The activity of the enzyme was strongly effected by the steric bulkiness of the substituents at the 3-position of the substrates, and the corresponding amides were obtained in high yields from the nitriles with free hydroxyl groups at the 3- and 4-positions.",
keywords = "2,5-anhydro-D- allononitrile, 2,5-anhydro-D-allonamide, hydantocidin, nitrile hydratase, Rhodococcus rhodochrous IFO 15564",
author = "Masahiro Yokoyama and Yuko Nakatsuka and Takeshi Sugai and Hiromichi Ohta",
year = "1996",
month = "9",
language = "English",
volume = "60",
pages = "1540--1542",
journal = "Bioscience, Biotechnology and Biochemistry",
issn = "0916-8451",
publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",
number = "9",

}

TY - JOUR

T1 - Action of nitrile hydratase from Rhodococcus rhodochrous IFO 15564 on derivatives of 2,5-anhydro-D-allononitrile

AU - Yokoyama, Masahiro

AU - Nakatsuka, Yuko

AU - Sugai, Takeshi

AU - Ohta, Hiromichi

PY - 1996/9

Y1 - 1996/9

N2 - The conversion of 2,5-anhydro-D-allononitrile derivatives by a nitrile hydratase from Rhodococcus rhodochrous IFO 15564 was studied. The activity of the enzyme was strongly effected by the steric bulkiness of the substituents at the 3-position of the substrates, and the corresponding amides were obtained in high yields from the nitriles with free hydroxyl groups at the 3- and 4-positions.

AB - The conversion of 2,5-anhydro-D-allononitrile derivatives by a nitrile hydratase from Rhodococcus rhodochrous IFO 15564 was studied. The activity of the enzyme was strongly effected by the steric bulkiness of the substituents at the 3-position of the substrates, and the corresponding amides were obtained in high yields from the nitriles with free hydroxyl groups at the 3- and 4-positions.

KW - 2,5-anhydro-D- allononitrile

KW - 2,5-anhydro-D-allonamide

KW - hydantocidin

KW - nitrile hydratase

KW - Rhodococcus rhodochrous IFO 15564

UR - http://www.scopus.com/inward/record.url?scp=0029771980&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029771980&partnerID=8YFLogxK

M3 - Article

VL - 60

SP - 1540

EP - 1542

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

IS - 9

ER -