Activated leukocytes and endothelial cells enhance retention of ultrasound contrast microspheres containing perfluoropropane in inflamed venules

Takanori Yasu, Yigal Greener, Edward Jablonski, Anne L. Killam, Shunichi Fukuda, Makoto Suematsu, Shinichiro J. Tojo, Geert W. Schmid-Schönbein

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Purpose: To characterize the flow dynamics of albumin ultrasound contrast microspheres containing perfluoropropane (PFP) in normal and inflamed microvasculature. Materials and methods: Mesenteric microvessels of rats were examined after an intravenous injection of fluorocein-labeled erythrocytes or PFP microspheres by fluorescence intravital microscopy with and without local application of 10-8 M platelet activating factor (PAF) as an experimental form of inflammation. Results: All the microspheres passed freely through arterioles and capillaries. Mean velocities of the microspheres in each vessel were closely correlated with those of erythrocytes. Only a minor fraction of the microspheres was retained in the venules (≥0.1 s stoppage) by attachment to endothelial cells. The frequency of microsphere retention in venules was significantly enhanced by PAF (2.6±2.1%, P<0.01 vs. control), especially in regions with leukocyte adhesion. Treatment with a monoclonal antibody to intercellular adhesion molecule-1, P-selectin or the common leukocyte antigen inhibited PAF-induced microsphere retention in venules (P<0.05). In the inflamed microcirculation, a small subgroup of microspheres becomes attached to venular endothelial cells in regions with leukocyte adhesion via interaction among microspheres, activated leukocytes and endothelial cells via adhesion molecules. Conclusion: In inflamed microcirculation, a small subgroup of microspheres becomes attached to venular endothelial cells in regions with leukocyte adhesion via interaction among microspheres, activated leukocytes and endothelial cells via adhesion molecules. These results suggest that ultrasonography with microspheres has the potential to evaluate inflammatory site distribution as well as tissue perfusion.

Original languageEnglish
Pages (from-to)245-252
Number of pages8
JournalInternational Journal of Cardiology
Volume98
Issue number2
DOIs
Publication statusPublished - 2005 Feb 15

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perflutren
Venules
Microspheres
Leukocytes
Endothelial Cells
Platelet Activating Factor
Cell Adhesion Molecules
Microcirculation
Microvessels
Erythrocytes

Keywords

  • Contrast
  • Echocardiography
  • Leukocyte
  • Membrane adhesion
  • Microcirculation

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Activated leukocytes and endothelial cells enhance retention of ultrasound contrast microspheres containing perfluoropropane in inflamed venules. / Yasu, Takanori; Greener, Yigal; Jablonski, Edward; Killam, Anne L.; Fukuda, Shunichi; Suematsu, Makoto; Tojo, Shinichiro J.; Schmid-Schönbein, Geert W.

In: International Journal of Cardiology, Vol. 98, No. 2, 15.02.2005, p. 245-252.

Research output: Contribution to journalArticle

Yasu, Takanori ; Greener, Yigal ; Jablonski, Edward ; Killam, Anne L. ; Fukuda, Shunichi ; Suematsu, Makoto ; Tojo, Shinichiro J. ; Schmid-Schönbein, Geert W. / Activated leukocytes and endothelial cells enhance retention of ultrasound contrast microspheres containing perfluoropropane in inflamed venules. In: International Journal of Cardiology. 2005 ; Vol. 98, No. 2. pp. 245-252.
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abstract = "Purpose: To characterize the flow dynamics of albumin ultrasound contrast microspheres containing perfluoropropane (PFP) in normal and inflamed microvasculature. Materials and methods: Mesenteric microvessels of rats were examined after an intravenous injection of fluorocein-labeled erythrocytes or PFP microspheres by fluorescence intravital microscopy with and without local application of 10-8 M platelet activating factor (PAF) as an experimental form of inflammation. Results: All the microspheres passed freely through arterioles and capillaries. Mean velocities of the microspheres in each vessel were closely correlated with those of erythrocytes. Only a minor fraction of the microspheres was retained in the venules (≥0.1 s stoppage) by attachment to endothelial cells. The frequency of microsphere retention in venules was significantly enhanced by PAF (2.6±2.1{\%}, P<0.01 vs. control), especially in regions with leukocyte adhesion. Treatment with a monoclonal antibody to intercellular adhesion molecule-1, P-selectin or the common leukocyte antigen inhibited PAF-induced microsphere retention in venules (P<0.05). In the inflamed microcirculation, a small subgroup of microspheres becomes attached to venular endothelial cells in regions with leukocyte adhesion via interaction among microspheres, activated leukocytes and endothelial cells via adhesion molecules. Conclusion: In inflamed microcirculation, a small subgroup of microspheres becomes attached to venular endothelial cells in regions with leukocyte adhesion via interaction among microspheres, activated leukocytes and endothelial cells via adhesion molecules. These results suggest that ultrasonography with microspheres has the potential to evaluate inflammatory site distribution as well as tissue perfusion.",
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AU - Greener, Yigal

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AU - Killam, Anne L.

AU - Fukuda, Shunichi

AU - Suematsu, Makoto

AU - Tojo, Shinichiro J.

AU - Schmid-Schönbein, Geert W.

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