Activated platelets and endothelial cell interaction with neutrophils under flow conditions

S. Mine, T. Fujisaki, M. Suematsu, Y. Tanaka

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Objective. The mechanism of neutrophil adhesion to the endothelium during the earliest stages of acute inflammation, especially before the induction of adhesion molecules on endothelial cells, remains unknown. We studied the possible involvement of platelets in this process. Methods. Neutrophils were added to human umbilical vein-derived endothelial cells (HUVEC) with or without adherent platelets in the presence or absence of adhesion-blocking monoclonal antibodies (mAbs). Adhesion of neutrophils to HUVEC at dynamic flow conditions was assessed using a flow chamber. Results. 1) Thrombin-activated platelets adhered to resting HUVEC at dynamic flow conditions through platelet glycoprotein IIb/IIIa and RGD proteins. 2) Neutrophils tethered to P-selectin induced on thrombin-activated platelets, which were immobilized on HUVEC. 3) Activated neutrophils adhered, via LFA-1, to ICAM-1 on HUVEC. 4) Activated platelets induced interleukin (IL)-8 secretion by HUVEC. Conclusions. Immobilized platelets on the vessel wall with induced P-selectin on the surface biochemically and functionally promote the adhesion of neutrophils to endothelial cells.

Original languageEnglish
Pages (from-to)1085-1092
Number of pages8
JournalInternal Medicine
Volume40
Issue number11
DOIs
Publication statusPublished - 2001 Jan 1
Externally publishedYes

Keywords

  • Endothelium
  • GPIIb/IIIa
  • Integrin
  • Neutrophil
  • P-selectin
  • Platelet

ASJC Scopus subject areas

  • Internal Medicine

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