Activation-induced cytidine deaminase expression in CD4 + T cells is associated with a unique IL-10-producing subset that increases with age

Hongyan Qin, Keiichiro Suzuki, Mikiyo Nakata, Shunsuke Chikuma, Nakako Izumi, Le Thi Huong, Mikako Maruya, Sidonia Fagarasan, Meinrad Busslinger, Tasuku Honjo, Hitoshi Nagaoka

Research output: Contribution to journal › Article

34 Citations (Scopus)

Abstract

Activation-induced cytidine deaminase (AID), produced by the Aicda gene, is essential for the immunoglobulin gene (Ig) alterations that form immune memory. Using a Cre-mediated genetic system, we unexpectedly found CD4 + T cells that had expressed Aicda (exAID cells) as well as B cells. ExAID cells increased with age, reaching up to 25% of the CD4 + and B220 + cell populations. ExAID B cells remained IgM +, suggesting that class-switched memory B cells do not accumulate in the spleen. In T cells, AID was expressed in a subset that produced IFN-γ and IL-10 but little IL-4 or IL-17, and showed no evidence of genetic mutation. Interestingly, the endogenous Aicda expression in T cells was enhanced in the absence of B cells, indicating that the process is independent from the germinal center reaction. These results suggest that in addition to its roles in B cells, AID may have previously unappreciated roles in T-cell function or tumorigenesis.

Original languageEnglish
Article numbere29141
JournalPLoS One
Volume6
Issue number12
DOIs
Publication statusPublished - 2011 Dec 28
Externally publishedYes

Fingerprint

T-cells
interleukin-10
Interleukin-10
B-lymphocytes
B-Lymphocytes
T-lymphocytes
Cells
T-Lymphocytes
immunoglobulin genes
Genes
Immunoglobulin Genes
Germinal Center
Interleukin-17
Essential Genes
Data storage equipment
cells
interleukin-4
Interleukin-4
carcinogenesis
Immunoglobulin M

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Activation-induced cytidine deaminase expression in CD4 + T cells is associated with a unique IL-10-producing subset that increases with age. / Qin, Hongyan; Suzuki, Keiichiro; Nakata, Mikiyo; Chikuma, Shunsuke; Izumi, Nakako; Thi Huong, Le; Maruya, Mikako; Fagarasan, Sidonia; Busslinger, Meinrad; Honjo, Tasuku; Nagaoka, Hitoshi.

In: PLoS One, Vol. 6, No. 12, e29141, 28.12.2011.

Research output: Contribution to journal › Article

Qin, H, Suzuki, K, Nakata, M, Chikuma, S, Izumi, N, Thi Huong, L, Maruya, M, Fagarasan, S, Busslinger, M, Honjo, T & Nagaoka, H 2011, 'Activation-induced cytidine deaminase expression in CD4 + T cells is associated with a unique IL-10-producing subset that increases with age', PLoS One, vol. 6, no. 12, e29141. https://doi.org/10.1371/journal.pone.0029141
Qin H, Suzuki K, Nakata M, Chikuma S, Izumi N, Thi Huong L et al. Activation-induced cytidine deaminase expression in CD4 + T cells is associated with a unique IL-10-producing subset that increases with age. PLoS One. 2011 Dec 28;6(12). e29141. https://doi.org/10.1371/journal.pone.0029141
Qin, Hongyan ; Suzuki, Keiichiro ; Nakata, Mikiyo ; Chikuma, Shunsuke ; Izumi, Nakako ; Thi Huong, Le ; Maruya, Mikako ; Fagarasan, Sidonia ; Busslinger, Meinrad ; Honjo, Tasuku ; Nagaoka, Hitoshi. / Activation-induced cytidine deaminase expression in CD4 + T cells is associated with a unique IL-10-producing subset that increases with age. In: PLoS One. 2011 ; Vol. 6, No. 12.
@article{82d3d5915e87451ebc07c7c3c0ea75fe,
title = "Activation-induced cytidine deaminase expression in CD4 + T cells is associated with a unique IL-10-producing subset that increases with age",
abstract = "Activation-induced cytidine deaminase (AID), produced by the Aicda gene, is essential for the immunoglobulin gene (Ig) alterations that form immune memory. Using a Cre-mediated genetic system, we unexpectedly found CD4 + T cells that had expressed Aicda (exAID cells) as well as B cells. ExAID cells increased with age, reaching up to 25{\%} of the CD4 + and B220 + cell populations. ExAID B cells remained IgM +, suggesting that class-switched memory B cells do not accumulate in the spleen. In T cells, AID was expressed in a subset that produced IFN-γ and IL-10 but little IL-4 or IL-17, and showed no evidence of genetic mutation. Interestingly, the endogenous Aicda expression in T cells was enhanced in the absence of B cells, indicating that the process is independent from the germinal center reaction. These results suggest that in addition to its roles in B cells, AID may have previously unappreciated roles in T-cell function or tumorigenesis.",
author = "Hongyan Qin and Keiichiro Suzuki and Mikiyo Nakata and Shunsuke Chikuma and Nakako Izumi and {Thi Huong}, Le and Mikako Maruya and Sidonia Fagarasan and Meinrad Busslinger and Tasuku Honjo and Hitoshi Nagaoka",
year = "2011",
month = "12",
day = "28",
doi = "10.1371/journal.pone.0029141",
language = "English",
volume = "6",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "12",

}

TY - JOUR

T1 - Activation-induced cytidine deaminase expression in CD4 + T cells is associated with a unique IL-10-producing subset that increases with age

AU - Qin, Hongyan

AU - Suzuki, Keiichiro

AU - Nakata, Mikiyo

AU - Chikuma, Shunsuke

AU - Izumi, Nakako

AU - Thi Huong, Le

AU - Maruya, Mikako

AU - Fagarasan, Sidonia

AU - Busslinger, Meinrad

AU - Honjo, Tasuku

AU - Nagaoka, Hitoshi

PY - 2011/12/28

Y1 - 2011/12/28

N2 - Activation-induced cytidine deaminase (AID), produced by the Aicda gene, is essential for the immunoglobulin gene (Ig) alterations that form immune memory. Using a Cre-mediated genetic system, we unexpectedly found CD4 + T cells that had expressed Aicda (exAID cells) as well as B cells. ExAID cells increased with age, reaching up to 25% of the CD4 + and B220 + cell populations. ExAID B cells remained IgM +, suggesting that class-switched memory B cells do not accumulate in the spleen. In T cells, AID was expressed in a subset that produced IFN-γ and IL-10 but little IL-4 or IL-17, and showed no evidence of genetic mutation. Interestingly, the endogenous Aicda expression in T cells was enhanced in the absence of B cells, indicating that the process is independent from the germinal center reaction. These results suggest that in addition to its roles in B cells, AID may have previously unappreciated roles in T-cell function or tumorigenesis.

AB - Activation-induced cytidine deaminase (AID), produced by the Aicda gene, is essential for the immunoglobulin gene (Ig) alterations that form immune memory. Using a Cre-mediated genetic system, we unexpectedly found CD4 + T cells that had expressed Aicda (exAID cells) as well as B cells. ExAID cells increased with age, reaching up to 25% of the CD4 + and B220 + cell populations. ExAID B cells remained IgM +, suggesting that class-switched memory B cells do not accumulate in the spleen. In T cells, AID was expressed in a subset that produced IFN-γ and IL-10 but little IL-4 or IL-17, and showed no evidence of genetic mutation. Interestingly, the endogenous Aicda expression in T cells was enhanced in the absence of B cells, indicating that the process is independent from the germinal center reaction. These results suggest that in addition to its roles in B cells, AID may have previously unappreciated roles in T-cell function or tumorigenesis.

UR - http://www.scopus.com/inward/record.url?scp=84555203265&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84555203265&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0029141

DO - 10.1371/journal.pone.0029141

M3 - Article

C2 - 22216188

AN - SCOPUS:84555203265

VL - 6

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 12

M1 - e29141

ER -

Access to Document