Activation-induced cytidine deaminase expression in CD4 + T cells is associated with a unique IL-10-producing subset that increases with age

Hongyan Qin, Keiichiro Suzuki, Mikiyo Nakata, Shunsuke Chikuma, Nakako Izumi, Le Thi Huong, Mikako Maruya, Sidonia Fagarasan, Meinrad Busslinger, Tasuku Honjo, Hitoshi Nagaoka

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Abstract

Activation-induced cytidine deaminase (AID), produced by the Aicda gene, is essential for the immunoglobulin gene (Ig) alterations that form immune memory. Using a Cre-mediated genetic system, we unexpectedly found CD4 + T cells that had expressed Aicda (exAID cells) as well as B cells. ExAID cells increased with age, reaching up to 25% of the CD4 + and B220 + cell populations. ExAID B cells remained IgM +, suggesting that class-switched memory B cells do not accumulate in the spleen. In T cells, AID was expressed in a subset that produced IFN-γ and IL-10 but little IL-4 or IL-17, and showed no evidence of genetic mutation. Interestingly, the endogenous Aicda expression in T cells was enhanced in the absence of B cells, indicating that the process is independent from the germinal center reaction. These results suggest that in addition to its roles in B cells, AID may have previously unappreciated roles in T-cell function or tumorigenesis.

Original languageEnglish
Article numbere29141
JournalPLoS One
Volume6
Issue number12
DOIs
Publication statusPublished - 2011 Dec 28
Externally publishedYes

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T-cells
interleukin-10
Interleukin-10
B-lymphocytes
B-Lymphocytes
T-lymphocytes
Cells
T-Lymphocytes
immunoglobulin genes
Genes
Immunoglobulin Genes
Germinal Center
Interleukin-17
Essential Genes
Data storage equipment
cells
interleukin-4
Interleukin-4
carcinogenesis
Immunoglobulin M

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Activation-induced cytidine deaminase expression in CD4 + T cells is associated with a unique IL-10-producing subset that increases with age. / Qin, Hongyan; Suzuki, Keiichiro; Nakata, Mikiyo; Chikuma, Shunsuke; Izumi, Nakako; Thi Huong, Le; Maruya, Mikako; Fagarasan, Sidonia; Busslinger, Meinrad; Honjo, Tasuku; Nagaoka, Hitoshi.

In: PLoS One, Vol. 6, No. 12, e29141, 28.12.2011.

Research output: Contribution to journalArticle

Qin, H, Suzuki, K, Nakata, M, Chikuma, S, Izumi, N, Thi Huong, L, Maruya, M, Fagarasan, S, Busslinger, M, Honjo, T & Nagaoka, H 2011, 'Activation-induced cytidine deaminase expression in CD4 + T cells is associated with a unique IL-10-producing subset that increases with age', PLoS One, vol. 6, no. 12, e29141. https://doi.org/10.1371/journal.pone.0029141
Qin, Hongyan ; Suzuki, Keiichiro ; Nakata, Mikiyo ; Chikuma, Shunsuke ; Izumi, Nakako ; Thi Huong, Le ; Maruya, Mikako ; Fagarasan, Sidonia ; Busslinger, Meinrad ; Honjo, Tasuku ; Nagaoka, Hitoshi. / Activation-induced cytidine deaminase expression in CD4 + T cells is associated with a unique IL-10-producing subset that increases with age. In: PLoS One. 2011 ; Vol. 6, No. 12.
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