Activation-induced cytidine deaminase expression in CD4 + T cells is associated with a unique IL-10-producing subset that increases with age

Hongyan Qin, Keiichiro Suzuki, Mikiyo Nakata, Shunsuke Chikuma, Nakako Izumi, Le Thi Huong, Mikako Maruya, Sidonia Fagarasan, Meinrad Busslinger, Tasuku Honjo, Hitoshi Nagaoka

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Abstract

Activation-induced cytidine deaminase (AID), produced by the Aicda gene, is essential for the immunoglobulin gene (Ig) alterations that form immune memory. Using a Cre-mediated genetic system, we unexpectedly found CD4 + T cells that had expressed Aicda (exAID cells) as well as B cells. ExAID cells increased with age, reaching up to 25% of the CD4 + and B220 + cell populations. ExAID B cells remained IgM +, suggesting that class-switched memory B cells do not accumulate in the spleen. In T cells, AID was expressed in a subset that produced IFN-γ and IL-10 but little IL-4 or IL-17, and showed no evidence of genetic mutation. Interestingly, the endogenous Aicda expression in T cells was enhanced in the absence of B cells, indicating that the process is independent from the germinal center reaction. These results suggest that in addition to its roles in B cells, AID may have previously unappreciated roles in T-cell function or tumorigenesis.

Original languageEnglish
Article numbere29141
JournalPloS one
Volume6
Issue number12
DOIs
Publication statusPublished - 2011 Dec 28

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Qin, H., Suzuki, K., Nakata, M., Chikuma, S., Izumi, N., Thi Huong, L., Maruya, M., Fagarasan, S., Busslinger, M., Honjo, T., & Nagaoka, H. (2011). Activation-induced cytidine deaminase expression in CD4 + T cells is associated with a unique IL-10-producing subset that increases with age. PloS one, 6(12), [e29141]. https://doi.org/10.1371/journal.pone.0029141