Activation of apoptosis by caspase-3-dependent specific RelB cleavage in anticancer agent-treated cancer cells: Involvement of positive feedback mechanism

Mizuki Kuboki, Ayumi Ito, Siro Simizu, Kazuo Umezawa

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

DTCM-glutarimide (DTCM-G) is a newly found anti-inflammatory agent. In the course of experiments with lymphoma cells, we found that DTCM-G induced specific RelB cleavage. Anticancer agent vinblastine also induced the specific RelB cleavage in human fibrosarcoma HT1080 cells. The site-directed mutagenesis analysis revealed that the Asp205 site in RelB was specifically cleaved possibly by caspase-3 in vinblastine-treated HT1080 cells. Moreover, the cells stably overexpressing RelB Asp205Ala were resistant to vinblastine-induced apoptosis. Thus, the specific Asp205 cleavage of RelB by caspase-3 would be involved in the apoptosis induction by anticancer agents, which would provide the positive feedback mechanism.

Original languageEnglish
Pages (from-to)810-814
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume456
Issue number3
DOIs
Publication statusPublished - 2015 Jan 16

Keywords

  • Apoptosis
  • Caspase-3
  • DTCM-glutarimide
  • RelB
  • Vinblastine

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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