Activation of extracellular signal-regulated kinase in the trigeminal ganglion following both treatment of the dura mater with capsaicin and cortical spreading depression

Tatsuo Iwashita, Toshihiko Shimizu, Mamoru Shibata, Haruki Toriumi, Taeko Ebine, Megumi Funakubo, Norihiro Suzuki

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Extracellular signal-regulated kinase (ERK) is known to be phosphorylated after exposure to noxious stimuli. In this study, we investigated the response in the dura mater to nociceptive stimulation, which is thought to be responsible for the pathogenesis of headaches, including migraines. We also examined the level of ERK phosphorylation in the trigeminal ganglion following cortical spreading depression (CSD), which is thought to play an important role in migraine pathophysiology. Western blot and immunohistochemical analyses showed a significant increase in the ERK phosphorylation levels 3. min following an application of 10. mM capsaicin to the dura mater. This increase was inhibited after an application of the TRPV1 antagonist capsazepine or a MEK inhibitor. An immunohistochemical analysis revealed that most of the small-sized trigeminal ganglion neurons with TRPV1-immunoreactivity that innervate the dura mater exhibited pERK-immunoreactivity, suggesting that these neurons had responded to nociceptive stimulation. CSD increased the level of ERK phosphorylation 30. min after its elicitation, and this response was inhibited by a prior intraventricular administration of TRPV1 antagonist. These results indicate that CSD can activate dural TRPV1 to send nociceptive signals to the trigeminal system, and they provide important clues regarding the relationship between CSD and the trigeminovascular system.

Original languageEnglish
Pages (from-to)110-119
Number of pages10
JournalNeuroscience Research
Volume77
Issue number1-2
DOIs
Publication statusPublished - 2013 Sep

Fingerprint

Cortical Spreading Depression
Dura Mater
Trigeminal Ganglion
Capsaicin
Extracellular Signal-Regulated MAP Kinases
Phosphorylation
Migraine Disorders
Neurons
Mitogen-Activated Protein Kinase Kinases
Western Blotting

Keywords

  • Cortical spreading depression
  • ERK
  • Trigeminal ganglion
  • TRPV1

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Activation of extracellular signal-regulated kinase in the trigeminal ganglion following both treatment of the dura mater with capsaicin and cortical spreading depression. / Iwashita, Tatsuo; Shimizu, Toshihiko; Shibata, Mamoru; Toriumi, Haruki; Ebine, Taeko; Funakubo, Megumi; Suzuki, Norihiro.

In: Neuroscience Research, Vol. 77, No. 1-2, 09.2013, p. 110-119.

Research output: Contribution to journalArticle

Iwashita, Tatsuo ; Shimizu, Toshihiko ; Shibata, Mamoru ; Toriumi, Haruki ; Ebine, Taeko ; Funakubo, Megumi ; Suzuki, Norihiro. / Activation of extracellular signal-regulated kinase in the trigeminal ganglion following both treatment of the dura mater with capsaicin and cortical spreading depression. In: Neuroscience Research. 2013 ; Vol. 77, No. 1-2. pp. 110-119.
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