Activation of heparanase by ultraviolet B irradiation leads to functional loss of basement membrane at the dermal-epidermal junction in human skin

Shunsuke Iriyama, Yukiko Matsunaga, Kazuhiro Takahashi, Kyoichi Matsuzaki, Norio Kumagai, Satoshi Amano

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20 Citations (Scopus)


Recently, we reported that heparanase plays important roles in barrier-disrupted skin, leading to increased interaction of growth factors between epidermis and dermis and facilitating various cutaneous changes, including epidermal hyperplasia and wrinkle formation. However, the role of heparanase in sun-exposed skin remains unknown. Here, we show that heparanase in human keratinocytes is activated by ultraviolet B (UVB) exposure and that heparan sulfate of perlecan is markedly degraded in UVB-irradiated human skin. The degradation of heparan sulfate resulted in a marked reduction of binding activity of the basement membrane for vascular endothelial growth factor, fibroblast growth factor-2 and -7 at the dermal-epidermal junction. Degradation of heparan sulfate was observed not only in acutely UVB-irradiated skin, but also in skin chronically exposed to sun. Interestingly, heparan sulfate was found to be degraded in sun-exposed skin, but not in sun-protected skin. These findings suggest that chronic UVB exposure activates heparanase, leading to degradation of heparan sulfate in the basement membrane and increased growth factor interaction between epidermis and dermis. These changes may facilitate photo-aging.

Original languageEnglish
Pages (from-to)253-261
Number of pages9
JournalArchives of Dermatological Research
Issue number4
Publication statusPublished - 2011 May 1



  • Aging
  • Basement membrane
  • Heparanase
  • Keratinocyte

ASJC Scopus subject areas

  • Dermatology

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