Activation of human neutrophils by Arg-Gly-Asp-Ser immobilized on microspheres

Y. Kasuya, Keiji Fujimoto, M. Miyamoto, H. Kawaguchi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The adhesive interaction of cells with extracellular matrix components is essential for a variety of cellular functions, and is frequently mediated by a tetra peptide, Arg-Gly-Asp-Ser (RGDS), located within fibronectin and other proteins. In this study, the RGDS-mediated activation of polymorphonuclear leukocytes accompanied by phagocytosis was investigated using monodisperse polymeric microspheres carrying RGDS. The parent and Arg-Gly-Glu-Ser (RGES)- carrying microspheres, which have no adhesion activity, were employed as controls. The ingestion of microspheres into PMN was not enhanced by immobilizing RGDS. However, PMNs exhibited unique oxygen consumption and enhanced liberation of reactive oxygen when RGDS-carrying microspheres were phagocytosed. These PMN responses disappeared with the addition of soluble RGDS. Furthermore, cytochalasin D, which inhibits actin polymerization, showed a marked inhibitory effect on oxygen consumption in the RGDS-carrying microsphere system, as compared with those in other systems. These findings show that RGDS-carrying microspheres induced the biospecific activation of PMNs by the signal transduction via RGDS-integrin binding without alteration in the degree of phagocytosis.

Original languageEnglish
Pages (from-to)397-404
Number of pages8
JournalJournal of Biomedical Materials Research
Volume28
Issue number3
Publication statusPublished - 1994 Mar

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Microspheres
Chemical activation
Oxygen
Cytochalasin D
Signal transduction
Fibronectins
Integrins
Peptides
Actins
Adhesives
Adhesion
Polymerization
Proteins

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

Activation of human neutrophils by Arg-Gly-Asp-Ser immobilized on microspheres. / Kasuya, Y.; Fujimoto, Keiji; Miyamoto, M.; Kawaguchi, H.

In: Journal of Biomedical Materials Research, Vol. 28, No. 3, 03.1994, p. 397-404.

Research output: Contribution to journalArticle

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