Activation of matrix metalloproteinase-2 in head and neck squamous cell carcinoma: Studies of clinical samples and in vitro cell lines co-cultured with fibroblasts

Yutaka Tokumaru, Masato Fujii, Yoshihide Otani, Kaori Kameyama, Yorihisa Imanishi, Naoki Igarashi, Jin Kanzaki

Research output: Contribution to journalArticle

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Abstract

We undertook this present study to investigate the activation of matrix metalloproteinase-2 (MMP-2) in human head and neck squamous cell carcinomas (HNSCC) tissues and cell lines. Gelatinolytic activities of active MMP-2 were significantly higher in carcinoma samples than in normal portions. Furthermore, the activation ratio of proMMP-2 significantly correlated with cervical lymph node metastasis. In vitro studies revealed an HNSCC cell line, HEp-2, to produce neither the pro form nor the active form of MMP-2, but human fibroblasts were found to produce proMMP-2. However, coculture of HEp-2 cells with fibroblasts resulted in the production of not only proMMP-2 but also activeMMP-2 in the culture medium. Northern blot analysis revealed a stronger expression of membrane-type 1 matrix metalloproteinase (MT1-MMP), which is a specific activator of MMP-2, mRNA in HEp-2 cells than in fibroblasts. These results suggest the activation of proMMP-2 as an important event in the process of HNSCC metastasis. They also suggest MMP-2 is secreted in its pro form by stromal fibroblasts surrounding the cancer cells and activated by MT1-MMP localized on the cancer cells. (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)15-21
Number of pages7
JournalCancer Letters
Volume150
Issue number1
DOIs
Publication statusPublished - 2000 Mar 13

Fingerprint

Matrix Metalloproteinase 2
Fibroblasts
Matrix Metalloproteinase 14
Cell Line
Neoplasm Metastasis
Coculture Techniques
Northern Blotting
Culture Media
Neoplasms
Lymph Nodes
Cell Membrane
Carcinoma
Messenger RNA
In Vitro Techniques
progelatinase
Carcinoma, squamous cell of head and neck
Clinical Studies
human MMP2 protein

Keywords

  • Fibroblast
  • Head and neck carcinoma
  • Matrix metalloproteinase-2
  • Membrane-type 1 matrix metalloproteinase

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

Cite this

Activation of matrix metalloproteinase-2 in head and neck squamous cell carcinoma : Studies of clinical samples and in vitro cell lines co-cultured with fibroblasts. / Tokumaru, Yutaka; Fujii, Masato; Otani, Yoshihide; Kameyama, Kaori; Imanishi, Yorihisa; Igarashi, Naoki; Kanzaki, Jin.

In: Cancer Letters, Vol. 150, No. 1, 13.03.2000, p. 15-21.

Research output: Contribution to journalArticle

Tokumaru, Yutaka ; Fujii, Masato ; Otani, Yoshihide ; Kameyama, Kaori ; Imanishi, Yorihisa ; Igarashi, Naoki ; Kanzaki, Jin. / Activation of matrix metalloproteinase-2 in head and neck squamous cell carcinoma : Studies of clinical samples and in vitro cell lines co-cultured with fibroblasts. In: Cancer Letters. 2000 ; Vol. 150, No. 1. pp. 15-21.
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