Activation of NG2-positive oligodendrocyte progenitor cells during post-ischemic reperfusion in the rat brain

K. Tanaka, S. Nogawa, Daisuke Ito, Shigeaki Suzuki, T. Dembo, A. Kosakai, Y. Fukuuchi

Research output: Contribution to journalArticle

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Abstract

This study examines the alteration of oligodendrocyte progenitor cells which express membrane NG2 chondroitin sulfate proteoglycan after focal ischemia in the rat brain. Adult male Sprague-Dawley rats were subjected to 90 min occlusion of the middle cerebral artery, followed by reperfusion time of up to 2 weeks. The distribution and morphological changes in NG2-positive oligodendrocyte progenitor cells were immunohistochemically examined. Stellate-shaped NG2-positive cells with multiple branched processes were detected in both the gray and white matter of normal brain. After 2 weeks of reperfusion, NG2-positive cells in the area surrounding the infarction site (peri-infarct area) clearly showed enlarged cell bodies with hypertrophied processes. These stained strongly for NG2. Although the number of NG2-positive cells was increased significantly in the peri-infarct area, it decreased markedly in the infarct core compared to controls. Double immunostaining revealed that these NG2-positive cells were neither astrocytes nor microglia, but NG2-positive oligodendrocyte progenitor cells. These progenitor cells are known to differentiate into oligodendrocytes. As such, this upregulation of NG2 expression may be an adaptive mechanism attempting to remyelinate rat brain tissue after ischemic insult. Only further study will elucidate this hypothesis.

Original languageEnglish
Pages (from-to)2169-2174
Number of pages6
JournalNeuroReport
Volume12
Issue number10
Publication statusPublished - 2001 Jul 20

Fingerprint

Oligodendroglia
Reperfusion
Stem Cells
Brain
Chondroitin Sulfate Proteoglycans
Middle Cerebral Artery Infarction
Microglia
Astrocytes
Infarction
Sprague Dawley Rats
Up-Regulation
Ischemia
Membranes

Keywords

  • Cerebral infarction
  • Chondroitin sulfate
  • Myelination
  • Oligodendrocyte
  • Reperfusion

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Tanaka, K., Nogawa, S., Ito, D., Suzuki, S., Dembo, T., Kosakai, A., & Fukuuchi, Y. (2001). Activation of NG2-positive oligodendrocyte progenitor cells during post-ischemic reperfusion in the rat brain. NeuroReport, 12(10), 2169-2174.

Activation of NG2-positive oligodendrocyte progenitor cells during post-ischemic reperfusion in the rat brain. / Tanaka, K.; Nogawa, S.; Ito, Daisuke; Suzuki, Shigeaki; Dembo, T.; Kosakai, A.; Fukuuchi, Y.

In: NeuroReport, Vol. 12, No. 10, 20.07.2001, p. 2169-2174.

Research output: Contribution to journalArticle

Tanaka, K, Nogawa, S, Ito, D, Suzuki, S, Dembo, T, Kosakai, A & Fukuuchi, Y 2001, 'Activation of NG2-positive oligodendrocyte progenitor cells during post-ischemic reperfusion in the rat brain', NeuroReport, vol. 12, no. 10, pp. 2169-2174.
Tanaka, K. ; Nogawa, S. ; Ito, Daisuke ; Suzuki, Shigeaki ; Dembo, T. ; Kosakai, A. ; Fukuuchi, Y. / Activation of NG2-positive oligodendrocyte progenitor cells during post-ischemic reperfusion in the rat brain. In: NeuroReport. 2001 ; Vol. 12, No. 10. pp. 2169-2174.
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