Activin A stimulates mitogenesis in Swiss 3T3 fibroblasts without activation of mitogen-activated protein kinases

Takeshi Sakurai, Yoichiro Abe, Yoshitoshi Kasuya, Noriko Takuwa, Reiko Shiba, Takayuki Yamashita, Takeshi Endo, Katsutoshi Goto

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Abstract

Activin A stimulated DNA synthesis and transient c-fos expression in quiescent Swiss 3T3 fibroblasts. The activin A-induced DNA synthesis was dose-dependent with a half-maximal effect obtained at 0.3 nM. The maximal response obtained at 10 nM was comparable with that induced by 5 ng/ml basic fibroblast growth factor, Swiss 3T3 fibroblasts expressed abundant high affinity binding sites for 125I-labeled activin A with a K(d) value of 0.63 nM and the number of binding sites at 24,000/cell. Northern blot analysis revealed that Swiss 3T3 fibroblasts express a high level of type II activin receptor mRNA. In an attempt to elucidate the mechanism of mitogenic action of activin A2 we examined the effect of activin A on mitogen- activated protein kinase activation. Unexpectedly, however, activin A did not induce kinase activation under conditions in which basic fibroblast growth factor and endothelin-I at similar or even less potent mitogenic concentrations did. Furthermore, activin A did not induce phosphorylation of the Erk2 species of mitogen-activated protein kinase. These observations strongly suggest that the activation of mitogen-activated protein kinase is not a necessary step for activin A-induced DNA synthesis in Swiss 3T3 fibroblasts.

Original languageEnglish
Pages (from-to)14118-14122
Number of pages5
JournalJournal of Biological Chemistry
Volume269
Issue number19
Publication statusPublished - 1994 May 13
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Sakurai, T., Abe, Y., Kasuya, Y., Takuwa, N., Shiba, R., Yamashita, T., Endo, T., & Goto, K. (1994). Activin A stimulates mitogenesis in Swiss 3T3 fibroblasts without activation of mitogen-activated protein kinases. Journal of Biological Chemistry, 269(19), 14118-14122.