Activity-dependent changes in impulse conduction of single human motor axons: A stimulated single fiber electromyography study

Yu ichi Noto, Sonoko Misawa, Kazuaki Kanai, Yasunori Sato, Kazumoto Shibuya, Sagiri Isose, Saiko Nasu, Yukari Sekiguchi, Yumi Fujimaki, Shigeki Ohmori, Masanori Nakagawa, Satoshi Kuwabara

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Abstract

Objective: The aim of this study is to develop a novel method to assess activity-dependent hyperpolarization in human single motor axons at a constant stimulus frequency by using intra-muscular axonal stimulating single fiber electromyography (s-SFEMG). Methods: We performed s-SFEMG in the extensor digitorum communis (EDC) muscle of 10 normal subjects, and measured changes in latencies for single muscle fiber action potentials (MAPs) during 500 stimuli delivered at 5, 10 and 20. Hz. The data were analyzed with a repeated measurement analysis, and multiple comparisons were performed. Results: A total of 585 MAPs were examined at 5. Hz (n=190), 10. Hz (n=210), and 20. Hz (n=185) steady stimulation. There was a progressive linear prolongation of latencies, as the stimulus rate increased (F=95.6, p<0.001); the least square means (SEM) of latency change were 100.7 (0.28)% at 5. Hz, 102.3 (0.27)% at 10. Hz and 105.3 (0.28)% at 20. Hz. There were statistically significant differences between frequencies by Tukey-Kramer's method. Despite the significant latency prolongation, no activity-dependent conduction block developed. A 20. Hz electric stimulation to intramuscular axons was well-tolerated in all the subjects. Conclusions: Tetanic stimulation at a constant rate results in significant latency increase in single human motor axons, the extent of which depends on the stimulus frequency. The findings imply that physiological discharge rates will activate the Na +/K + pump and thereby produce axonal hyperpolarization in single motor axons. Significance: This technique may detect activity-dependent conduction block if the safety margin of impulse transmission is significantly reduced by demyelination or increased branching due to collateral sprouting in a variety of neuromuscular disorders.

Original languageEnglish
Pages (from-to)2512-2517
Number of pages6
JournalClinical Neurophysiology
Volume122
Issue number12
DOIs
Publication statusPublished - 2011 Dec 1

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Keywords

  • Activity-dependent conduction block
  • Activity-dependent hyperpolarization
  • Single fiber electromyography
  • Single motor axon

ASJC Scopus subject areas

  • Sensory Systems
  • Neurology
  • Clinical Neurology
  • Physiology (medical)

Cite this

Noto, Y. I., Misawa, S., Kanai, K., Sato, Y., Shibuya, K., Isose, S., Nasu, S., Sekiguchi, Y., Fujimaki, Y., Ohmori, S., Nakagawa, M., & Kuwabara, S. (2011). Activity-dependent changes in impulse conduction of single human motor axons: A stimulated single fiber electromyography study. Clinical Neurophysiology, 122(12), 2512-2517. https://doi.org/10.1016/j.clinph.2011.05.005