Activity-Dependent Proteolytic Cleavage of Neuroligin-1

Kunimichi Suzuki, Yukari Hayashi, Soichiro Nakahara, Hiroshi Kumazaki, Johannes Prox, Keisuke Horiuchi, Mingshuo Zeng, Shun Tanimura, Yoshitake Nishiyama, Satoko Osawa, Atsuko Sehara-Fujisawa, Paul Saftig, Satoshi Yokoshima, Tohru Fukuyama, Norio Matsuki, Ryuta Koyama, Taisuke Tomita, Takeshi Iwatsubo

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

Neuroligin (NLG), a postsynaptic adhesion molecule, is involved in the formation of synapses by binding to a cognate presynaptic ligand, neurexin. Here we report that neuroligin-1 (NLG1) undergoes ectodomain shedding at the juxtamembrane stalk region to generate a secreted form of NLG1 and a membrane-tethered C-terminal fragment (CTF) in adult rat brains in vivo as well as in neuronal cultures. Pharmacological and genetic studies identified ADAM10 as the major protease responsible for NLG1 shedding, the latter being augmented by synaptic NMDA receptor activation or interaction with soluble neurexin ligands. NLG1-CTF was subsequently cleaved by presenilin/γ-secretase. Secretion of soluble NLG1 was significantly upregulated under a prolonged epileptic seizure condition, and inhibition of NLG1 shedding led to an increase in numbers of dendritic spines in neuronal cultures. Collectively, neuronal activity-dependent proteolytic processing of NLG1 may negatively regulate the remodeling of spines at excitatory synapses.

Original languageEnglish
Pages (from-to)410-422
Number of pages13
JournalNeuron
Volume76
Issue number2
DOIs
Publication statusPublished - 2012 Oct 18

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Synapses
Presenilins
Ligands
Amyloid Precursor Protein Secretases
Dendritic Spines
Neurotransmitter Receptor
neuroligin 1
N-Methyl-D-Aspartate Receptors
Epilepsy
Spine
Peptide Hydrolases
Pharmacology
Membranes
Brain

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Suzuki, K., Hayashi, Y., Nakahara, S., Kumazaki, H., Prox, J., Horiuchi, K., ... Iwatsubo, T. (2012). Activity-Dependent Proteolytic Cleavage of Neuroligin-1. Neuron, 76(2), 410-422. https://doi.org/10.1016/j.neuron.2012.10.003

Activity-Dependent Proteolytic Cleavage of Neuroligin-1. / Suzuki, Kunimichi; Hayashi, Yukari; Nakahara, Soichiro; Kumazaki, Hiroshi; Prox, Johannes; Horiuchi, Keisuke; Zeng, Mingshuo; Tanimura, Shun; Nishiyama, Yoshitake; Osawa, Satoko; Sehara-Fujisawa, Atsuko; Saftig, Paul; Yokoshima, Satoshi; Fukuyama, Tohru; Matsuki, Norio; Koyama, Ryuta; Tomita, Taisuke; Iwatsubo, Takeshi.

In: Neuron, Vol. 76, No. 2, 18.10.2012, p. 410-422.

Research output: Contribution to journalArticle

Suzuki, K, Hayashi, Y, Nakahara, S, Kumazaki, H, Prox, J, Horiuchi, K, Zeng, M, Tanimura, S, Nishiyama, Y, Osawa, S, Sehara-Fujisawa, A, Saftig, P, Yokoshima, S, Fukuyama, T, Matsuki, N, Koyama, R, Tomita, T & Iwatsubo, T 2012, 'Activity-Dependent Proteolytic Cleavage of Neuroligin-1', Neuron, vol. 76, no. 2, pp. 410-422. https://doi.org/10.1016/j.neuron.2012.10.003
Suzuki K, Hayashi Y, Nakahara S, Kumazaki H, Prox J, Horiuchi K et al. Activity-Dependent Proteolytic Cleavage of Neuroligin-1. Neuron. 2012 Oct 18;76(2):410-422. https://doi.org/10.1016/j.neuron.2012.10.003
Suzuki, Kunimichi ; Hayashi, Yukari ; Nakahara, Soichiro ; Kumazaki, Hiroshi ; Prox, Johannes ; Horiuchi, Keisuke ; Zeng, Mingshuo ; Tanimura, Shun ; Nishiyama, Yoshitake ; Osawa, Satoko ; Sehara-Fujisawa, Atsuko ; Saftig, Paul ; Yokoshima, Satoshi ; Fukuyama, Tohru ; Matsuki, Norio ; Koyama, Ryuta ; Tomita, Taisuke ; Iwatsubo, Takeshi. / Activity-Dependent Proteolytic Cleavage of Neuroligin-1. In: Neuron. 2012 ; Vol. 76, No. 2. pp. 410-422.
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