Acute cell death rate of vascular smooth muscle cells during or after short heating up to 20s ranging 50 to 60°C as a basic study of thermal angioplasty

Machiko Shinozuka, Natsumi Shimazaki, Emiyu Ogawa, Naoki Machida, Tsunenori Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We studied the relations between the time history of smooth muscle cells (SMCs) death rate and heating condition in vitro to clarify cell death mechanism in heating angioplasty, in particular under the condition in which intimal hyperplasia growth had been prevented in vivo swine experiment. A flow heating system on the microscope stage was used for the SMCs death rate measurement during or after the heating. The cells were loaded step-heating by heated flow using a heater equipped in a Photo-thermo dynamic balloon. The heating temperature was set to 37, 50-60°C. The SMCs death rate was calculated by a division of PI stained cell number by Hoechst33342 stained cell number. The SMCs death rate increased 5-10% linearly during 20 s with the heating. The SMCs death rate increased with duration up to 15 min after 5 s heating. Because fragmented nuclei were observed from approximately 5 min after the heating, we defined that acute necrosis and late necrosis were corresponded to within 5 min after the heating and over 5 min after the heating, respectively. This late necrosis is probably corresponding to apoptosis. The ratio of necrotic interaction divided the acute necrosis rate by the late necrosis was calculated based on this consideration as 1.3 under the particular condition in which intimal hyperplasia growth was prevented in vivo previous porcine experiment. We think that necrotic interaction rate is larger than expected rate to obtain intimal hyperplasia suppression.

Original languageEnglish
Title of host publicationOptical Interactions with Tissue and Cells XXV; and Terahertz for Biomedical Applications
PublisherSPIE
ISBN (Print)9780819498540
DOIs
Publication statusPublished - 2014 Jan 1
EventOptical Interactions with Tissue and Cells XXV; and Terahertz for Biomedical Applications - San Francisco, CA, United States
Duration: 2014 Feb 22014 Feb 4

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8941
ISSN (Print)1605-7422

Other

OtherOptical Interactions with Tissue and Cells XXV; and Terahertz for Biomedical Applications
CountryUnited States
CitySan Francisco, CA
Period14/2/214/2/4

Keywords

  • Apoptosis
  • Intimal hyperplasia
  • Necrosis
  • Short heating
  • Smooth muscle cell (SMC)

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Shinozuka, M., Shimazaki, N., Ogawa, E., Machida, N., & Arai, T. (2014). Acute cell death rate of vascular smooth muscle cells during or after short heating up to 20s ranging 50 to 60°C as a basic study of thermal angioplasty. In Optical Interactions with Tissue and Cells XXV; and Terahertz for Biomedical Applications [89410L] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8941). SPIE. https://doi.org/10.1117/12.2039040