Acute cell death rate of vascular smooth muscle cells during or after short heating up to 20s ranging 50 to 60°C as a basic study of thermal angioplasty

Machiko Shinozuka, Natsumi Shimazaki, Emiyu Ogawa, Naoki Machida, Tsunenori Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We studied the relations between the time history of smooth muscle cells (SMCs) death rate and heating condition in vitro to clarify cell death mechanism in heating angioplasty, in particular under the condition in which intimal hyperplasia growth had been prevented in vivo swine experiment. A flow heating system on the microscope stage was used for the SMCs death rate measurement during or after the heating. The cells were loaded step-heating by heated flow using a heater equipped in a Photo-thermo dynamic balloon. The heating temperature was set to 37, 50-60°C. The SMCs death rate was calculated by a division of PI stained cell number by Hoechst33342 stained cell number. The SMCs death rate increased 5-10% linearly during 20 s with the heating. The SMCs death rate increased with duration up to 15 min after 5 s heating. Because fragmented nuclei were observed from approximately 5 min after the heating, we defined that acute necrosis and late necrosis were corresponded to within 5 min after the heating and over 5 min after the heating, respectively. This late necrosis is probably corresponding to apoptosis. The ratio of necrotic interaction divided the acute necrosis rate by the late necrosis was calculated based on this consideration as 1.3 under the particular condition in which intimal hyperplasia growth was prevented in vivo previous porcine experiment. We think that necrotic interaction rate is larger than expected rate to obtain intimal hyperplasia suppression.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
PublisherSPIE
Volume8941
ISBN (Print)9780819498540
DOIs
Publication statusPublished - 2014
EventOptical Interactions with Tissue and Cells XXV; and Terahertz for Biomedical Applications - San Francisco, CA, United States
Duration: 2014 Feb 22014 Feb 4

Other

OtherOptical Interactions with Tissue and Cells XXV; and Terahertz for Biomedical Applications
CountryUnited States
CitySan Francisco, CA
Period14/2/214/2/4

Fingerprint

smooth muscle
muscle cells
Cell death
Vascular Smooth Muscle
Angioplasty
death
Heating
Smooth Muscle Myocytes
Muscle
Cell Death
Hot Temperature
Cells
heating
Mortality
necrosis
Tunica Intima
Necrosis
Hyperplasia
Swine
Cell Count

Keywords

  • Apoptosis
  • Intimal hyperplasia
  • Necrosis
  • Short heating
  • Smooth muscle cell (SMC)

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Shinozuka, M., Shimazaki, N., Ogawa, E., Machida, N., & Arai, T. (2014). Acute cell death rate of vascular smooth muscle cells during or after short heating up to 20s ranging 50 to 60°C as a basic study of thermal angioplasty. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 8941). [89410L] SPIE. https://doi.org/10.1117/12.2039040

Acute cell death rate of vascular smooth muscle cells during or after short heating up to 20s ranging 50 to 60°C as a basic study of thermal angioplasty. / Shinozuka, Machiko; Shimazaki, Natsumi; Ogawa, Emiyu; Machida, Naoki; Arai, Tsunenori.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8941 SPIE, 2014. 89410L.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Shinozuka, M, Shimazaki, N, Ogawa, E, Machida, N & Arai, T 2014, Acute cell death rate of vascular smooth muscle cells during or after short heating up to 20s ranging 50 to 60°C as a basic study of thermal angioplasty. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 8941, 89410L, SPIE, Optical Interactions with Tissue and Cells XXV; and Terahertz for Biomedical Applications, San Francisco, CA, United States, 14/2/2. https://doi.org/10.1117/12.2039040
Shinozuka M, Shimazaki N, Ogawa E, Machida N, Arai T. Acute cell death rate of vascular smooth muscle cells during or after short heating up to 20s ranging 50 to 60°C as a basic study of thermal angioplasty. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8941. SPIE. 2014. 89410L https://doi.org/10.1117/12.2039040
Shinozuka, Machiko ; Shimazaki, Natsumi ; Ogawa, Emiyu ; Machida, Naoki ; Arai, Tsunenori. / Acute cell death rate of vascular smooth muscle cells during or after short heating up to 20s ranging 50 to 60°C as a basic study of thermal angioplasty. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8941 SPIE, 2014.
@inproceedings{0ec384ce4cbb483e9db232bf206d5dfe,
title = "Acute cell death rate of vascular smooth muscle cells during or after short heating up to 20s ranging 50 to 60°C as a basic study of thermal angioplasty",
abstract = "We studied the relations between the time history of smooth muscle cells (SMCs) death rate and heating condition in vitro to clarify cell death mechanism in heating angioplasty, in particular under the condition in which intimal hyperplasia growth had been prevented in vivo swine experiment. A flow heating system on the microscope stage was used for the SMCs death rate measurement during or after the heating. The cells were loaded step-heating by heated flow using a heater equipped in a Photo-thermo dynamic balloon. The heating temperature was set to 37, 50-60°C. The SMCs death rate was calculated by a division of PI stained cell number by Hoechst33342 stained cell number. The SMCs death rate increased 5-10{\%} linearly during 20 s with the heating. The SMCs death rate increased with duration up to 15 min after 5 s heating. Because fragmented nuclei were observed from approximately 5 min after the heating, we defined that acute necrosis and late necrosis were corresponded to within 5 min after the heating and over 5 min after the heating, respectively. This late necrosis is probably corresponding to apoptosis. The ratio of necrotic interaction divided the acute necrosis rate by the late necrosis was calculated based on this consideration as 1.3 under the particular condition in which intimal hyperplasia growth was prevented in vivo previous porcine experiment. We think that necrotic interaction rate is larger than expected rate to obtain intimal hyperplasia suppression.",
keywords = "Apoptosis, Intimal hyperplasia, Necrosis, Short heating, Smooth muscle cell (SMC)",
author = "Machiko Shinozuka and Natsumi Shimazaki and Emiyu Ogawa and Naoki Machida and Tsunenori Arai",
year = "2014",
doi = "10.1117/12.2039040",
language = "English",
isbn = "9780819498540",
volume = "8941",
booktitle = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",

}

TY - GEN

T1 - Acute cell death rate of vascular smooth muscle cells during or after short heating up to 20s ranging 50 to 60°C as a basic study of thermal angioplasty

AU - Shinozuka, Machiko

AU - Shimazaki, Natsumi

AU - Ogawa, Emiyu

AU - Machida, Naoki

AU - Arai, Tsunenori

PY - 2014

Y1 - 2014

N2 - We studied the relations between the time history of smooth muscle cells (SMCs) death rate and heating condition in vitro to clarify cell death mechanism in heating angioplasty, in particular under the condition in which intimal hyperplasia growth had been prevented in vivo swine experiment. A flow heating system on the microscope stage was used for the SMCs death rate measurement during or after the heating. The cells were loaded step-heating by heated flow using a heater equipped in a Photo-thermo dynamic balloon. The heating temperature was set to 37, 50-60°C. The SMCs death rate was calculated by a division of PI stained cell number by Hoechst33342 stained cell number. The SMCs death rate increased 5-10% linearly during 20 s with the heating. The SMCs death rate increased with duration up to 15 min after 5 s heating. Because fragmented nuclei were observed from approximately 5 min after the heating, we defined that acute necrosis and late necrosis were corresponded to within 5 min after the heating and over 5 min after the heating, respectively. This late necrosis is probably corresponding to apoptosis. The ratio of necrotic interaction divided the acute necrosis rate by the late necrosis was calculated based on this consideration as 1.3 under the particular condition in which intimal hyperplasia growth was prevented in vivo previous porcine experiment. We think that necrotic interaction rate is larger than expected rate to obtain intimal hyperplasia suppression.

AB - We studied the relations between the time history of smooth muscle cells (SMCs) death rate and heating condition in vitro to clarify cell death mechanism in heating angioplasty, in particular under the condition in which intimal hyperplasia growth had been prevented in vivo swine experiment. A flow heating system on the microscope stage was used for the SMCs death rate measurement during or after the heating. The cells were loaded step-heating by heated flow using a heater equipped in a Photo-thermo dynamic balloon. The heating temperature was set to 37, 50-60°C. The SMCs death rate was calculated by a division of PI stained cell number by Hoechst33342 stained cell number. The SMCs death rate increased 5-10% linearly during 20 s with the heating. The SMCs death rate increased with duration up to 15 min after 5 s heating. Because fragmented nuclei were observed from approximately 5 min after the heating, we defined that acute necrosis and late necrosis were corresponded to within 5 min after the heating and over 5 min after the heating, respectively. This late necrosis is probably corresponding to apoptosis. The ratio of necrotic interaction divided the acute necrosis rate by the late necrosis was calculated based on this consideration as 1.3 under the particular condition in which intimal hyperplasia growth was prevented in vivo previous porcine experiment. We think that necrotic interaction rate is larger than expected rate to obtain intimal hyperplasia suppression.

KW - Apoptosis

KW - Intimal hyperplasia

KW - Necrosis

KW - Short heating

KW - Smooth muscle cell (SMC)

UR - http://www.scopus.com/inward/record.url?scp=84901760870&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84901760870&partnerID=8YFLogxK

U2 - 10.1117/12.2039040

DO - 10.1117/12.2039040

M3 - Conference contribution

AN - SCOPUS:84901760870

SN - 9780819498540

VL - 8941

BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

PB - SPIE

ER -