Acute reduction of neuronal RNA binding Elavl2 protein and Gap43 mRNA in mouse hippocampus after kainic acid treatment

Takafumi Ohtsuka, Masato Yano, Hideyuki Okano

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Activity-dependent gene regulation in neurons has been hypothesized to be under transcriptional control and to include dramatic increases in immediate early genes (IEGs) after neuronal activity. In addition, several reports have focused on post-transcriptional regulation, which could be mediated by neuronal post-transcriptional regulators, including RNA binding proteins (RNABPs). One such protein family is the neuronal Elavls (nElavls; Elavl2, Elavl3, and Elavl4), whose members are widely expressed in peripheral and central nervous system. Previous reports showed that Elavl3 and 4 are up-regulated following repeated stimulation such as during cocaine administration, a seizure, or a spatial discrimination task. In this study, we focused on Elavl2, a candidate gene for schizophrenia and studied its role in neuronal activity. First we found that Elavl2 has a cell-type specific expression pattern that is highly expressed in hippocampal CA3 pyramidal neurons and hilar interneurons using Elavl2 specific antibody. Second, unexpectedly, we discovered that the Elavl2 protein level in the hippocampus was acutely down-regulated for 3 h after a kainic acid (KA)-induced seizure in the hippocampal CA3 region. In addition, level of Gap43 mRNA, a target mRNA of Elavl2 is decreased 12 h after KA treatment, thus suggesting the involvement of Elavl2 in activity-dependent RNA regulation.

Original languageEnglish
Article number34476
Pages (from-to)46-51
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume466
Issue number1
DOIs
Publication statusPublished - 2015 Aug 24

Fingerprint

RNA-Binding Proteins
Kainic Acid
Neurons
Hippocampus
Seizures
Genes
Hippocampal CA3 Region
RNA
Messenger RNA
Immediate-Early Genes
Pyramidal Cells
Peripheral Nervous System
Neurology
Interneurons
Cocaine
Gene expression
Schizophrenia
Proteins
Central Nervous System
Antibodies

Keywords

  • Elavl2
  • Neuronal activity
  • Post-transcriptional control
  • RNA binding protein

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Acute reduction of neuronal RNA binding Elavl2 protein and Gap43 mRNA in mouse hippocampus after kainic acid treatment. / Ohtsuka, Takafumi; Yano, Masato; Okano, Hideyuki.

In: Biochemical and Biophysical Research Communications, Vol. 466, No. 1, 34476, 24.08.2015, p. 46-51.

Research output: Contribution to journalArticle

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