ADAM-17 associated with CD44 cleavage and metastasis in oral squamous cell carcinoma

Yasuo Takamune, Tetsuro Ikebe, Osamu Nagano, Hideki Nakayama, Kazutoshi Ota, Takehisa Obayashi, Hideyuki Saya, Masanori Shinohara

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

ADAM-17 (a disintegrin and metalloproteinase 17) is a membrane-anchored protein, which can cleave the ectodomain in a variety of transmembrane proteins. In the in vitro experiments with tumor cells, ADAM-17 is reported to cleave CD44, an adhesion molecule that interacts with hyaluronic acid, to promote tumor cell migration. In the present study, we examined ADAM-17 expression and CD44 cleavage in specimens from 50 patients diagnosed to have oral squamous cell carcinoma (SCC). Each specimen was divided into two pieces, one was studied by immunohistochemistry and the other was subjected to a Western blot. By coordinating the results of both analyses, ADAM-17 expression was evaluated to be high in 23 cases (46%). When CD44 cleavage was also studied by immunohistochemical staining as well as with Western blotting, CD44 cleavage was judged to be positive in 29 cases (58%). When the ADAM-17 expression level was compared with the CD44 cleavage state, most of the cases expressing high levels of ADAM-17 (87%) showed positive CD44 cleavage. The level of ADAM-17 expression was significantly correlated to the nodal metastasis and local recurrence in oral SCC. Our findings suggest that ADAM-17 is involved in CD44 cleavage and contributes to tumor progression in oral SCC.

Original languageEnglish
Pages (from-to)169-177
Number of pages9
JournalVirchows Archiv
Volume450
Issue number2
DOIs
Publication statusPublished - 2007 Feb 1

Keywords

  • ADAM-17
  • CD44
  • Squamous cell carcinoma
  • TACE

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

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