ADAMTS-1 cleaves a cartilage proteoglycan, aggrecan

Kouji Kuno, Yasunori Okada, Hiroto Kawashima, Hiroyuki Nakamura, Masayuki Miyasaka, Hiroshi Ohno, Kouji Matsushima

Research output: Contribution to journalArticle

211 Citations (Scopus)

Abstract

A disintegrin-like and metalloproteinase with thrombospondin type I motifs-1 (ADAMTS-1) is an extracellular matrix-anchored metalloproteinase. In this study we have demonstrated that ADAMTS-1 is able to cleave a major cartilage proteoglycan, aggrecan. N-terminal sequencing analysis of the cleavage product revealed that ADAMTS-1 cleaves the Glu1871-Leu1872 bond within the chondroitin sulfate attachment domain of aggrecan. In addition, deletional analysis demonstrated that the C-terminal spacer region of ADAMTS-1 is necessary to degrade aggrecan. These results suggest that ADAMTS-1 may be involved in the turnover of aggrecan in vivo. (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)241-245
Number of pages5
JournalFEBS Letters
Volume478
Issue number3
DOIs
Publication statusPublished - 2000 Aug 4

Fingerprint

Aggrecans
Cartilage
Proteoglycans
Metalloproteases
Thrombospondins
Disintegrins
Chondroitin Sulfates
Matrix Metalloproteinases
Extracellular Matrix

Keywords

  • Aggrecan
  • Cartilage degradation
  • Disintegrin and metalloproteinase thrombospondin
  • Extracellular matrix

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Kuno, K., Okada, Y., Kawashima, H., Nakamura, H., Miyasaka, M., Ohno, H., & Matsushima, K. (2000). ADAMTS-1 cleaves a cartilage proteoglycan, aggrecan. FEBS Letters, 478(3), 241-245. https://doi.org/10.1016/S0014-5793(00)01854-8

ADAMTS-1 cleaves a cartilage proteoglycan, aggrecan. / Kuno, Kouji; Okada, Yasunori; Kawashima, Hiroto; Nakamura, Hiroyuki; Miyasaka, Masayuki; Ohno, Hiroshi; Matsushima, Kouji.

In: FEBS Letters, Vol. 478, No. 3, 04.08.2000, p. 241-245.

Research output: Contribution to journalArticle

Kuno, K, Okada, Y, Kawashima, H, Nakamura, H, Miyasaka, M, Ohno, H & Matsushima, K 2000, 'ADAMTS-1 cleaves a cartilage proteoglycan, aggrecan', FEBS Letters, vol. 478, no. 3, pp. 241-245. https://doi.org/10.1016/S0014-5793(00)01854-8
Kuno K, Okada Y, Kawashima H, Nakamura H, Miyasaka M, Ohno H et al. ADAMTS-1 cleaves a cartilage proteoglycan, aggrecan. FEBS Letters. 2000 Aug 4;478(3):241-245. https://doi.org/10.1016/S0014-5793(00)01854-8
Kuno, Kouji ; Okada, Yasunori ; Kawashima, Hiroto ; Nakamura, Hiroyuki ; Miyasaka, Masayuki ; Ohno, Hiroshi ; Matsushima, Kouji. / ADAMTS-1 cleaves a cartilage proteoglycan, aggrecan. In: FEBS Letters. 2000 ; Vol. 478, No. 3. pp. 241-245.
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