ADAMTS-1 cleaves a cartilage proteoglycan, aggrecan

Kouji Kuno, Yasunori Okada, Hiroto Kawashima, Hiroyuki Nakamura, Masayuki Miyasaka, Hiroshi Ohno, Kouji Matsushima

Research output: Contribution to journalArticlepeer-review

215 Citations (Scopus)

Abstract

A disintegrin-like and metalloproteinase with thrombospondin type I motifs-1 (ADAMTS-1) is an extracellular matrix-anchored metalloproteinase. In this study we have demonstrated that ADAMTS-1 is able to cleave a major cartilage proteoglycan, aggrecan. N-terminal sequencing analysis of the cleavage product revealed that ADAMTS-1 cleaves the Glu1871-Leu1872 bond within the chondroitin sulfate attachment domain of aggrecan. In addition, deletional analysis demonstrated that the C-terminal spacer region of ADAMTS-1 is necessary to degrade aggrecan. These results suggest that ADAMTS-1 may be involved in the turnover of aggrecan in vivo. (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)241-245
Number of pages5
JournalFEBS Letters
Volume478
Issue number3
DOIs
Publication statusPublished - 2000 Aug 4

Keywords

  • Aggrecan
  • Cartilage degradation
  • Disintegrin and metalloproteinase thrombospondin
  • Extracellular matrix

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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