Adaptation to HIF1a deletion in hypoxic cancer cells by upregulation of GLUT14 and creatine metabolism

Alessandro Valli, Matteo Morotti, Christos E. Zois, Patrick K. Albers, Tomoyoshi Soga, Katharina Feldinger, Roman Fischer, Martin Frejno, Alan McIntyre, Esther Bridges, Syed Haider, Francesca M. Buffa, Dilair Baban, Miguel Rodriguez, Oscar Yanes, Hannah J. Whittington, Hannah A. Lake, Sevasti Zervou, Craig A. Lygate, Benedikt M. KesslerAdrian L. Harris

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Hypoxia-inducible factor 1a is a key regulator of the hypoxia response in normal and cancer tissues. It is well recognized to regulate glycolysis and is a target for therapy. However, how tumor cells adapt to grow in the absence of HIF1a is poorly understood and an important concept to understand for developing targeted therapies is the flexibility of the metabolic response to hypoxia via alternative pathways. We analyzed pathways that allow cells to survive hypoxic stress in the absence of HIF1a, using the HCT116 colon cancer cell line with deleted HIF1a versus control. Spheroids were used to provide a 3D model of metabolic gradients. We conducted a metabolomic, transcriptomic, and proteomic analysis and integrated the results. These showed surprisingly that in three-dimensional growth, a key regulatory step of glycolysis is Aldolase A rather than phosphofructokinase. Furthermore, glucose uptake could be maintained in hypoxia through upregulation of GLUT14, not previously recognized in this role. Finally, there was a marked adaptation and change of phosphocreatine energy pathways, which made the cells susceptible to inhibition of creatine metabolism in hypoxic conditions. Overall, our studies show a complex adaptation to hypoxia that can bypass HIF1a, but it is targetable and it provides new insight into the key metabolic pathways involved in cancer growth.

Original languageEnglish
Pages (from-to)1531-1544
Number of pages14
JournalMolecular Cancer Research
Volume17
Issue number7
DOIs
Publication statusPublished - 2019 Jul 1

ASJC Scopus subject areas

  • Molecular Biology
  • Oncology
  • Cancer Research

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    Valli, A., Morotti, M., Zois, C. E., Albers, P. K., Soga, T., Feldinger, K., Fischer, R., Frejno, M., McIntyre, A., Bridges, E., Haider, S., Buffa, F. M., Baban, D., Rodriguez, M., Yanes, O., Whittington, H. J., Lake, H. A., Zervou, S., Lygate, C. A., ... Harris, A. L. (2019). Adaptation to HIF1a deletion in hypoxic cancer cells by upregulation of GLUT14 and creatine metabolism. Molecular Cancer Research, 17(7), 1531-1544. https://doi.org/10.1158/1541-7786.MCR-18-0315