Adaptive optics with spatio-temporal lock-in detection for temporal focusing microscopy

Tomohiro Ishikawa, Keisuke Isobe, Kenta Inazawa, Kana Namiki, Atsushi Miyawaki, Fumihiko Kannari, Katsumi Midorikawa

Research output: Contribution to journalArticlepeer-review

Abstract

Wavefront distortion in temporal focusing microscopy (TFM) results in a distorted temporal profile of the excitation pulses owing to spatio-temporal coupling. Since the pulse duration is dramatically changed in the excitation volume, it is difficult to correct the temporal profile for a thick sample. Here, we demonstrate adaptive optics (AO) correction in a thick sample. We apply structured illumination microscopy (SIM) to an AO correction in wide-field TFM to decrease the change in the pulse duration in the signal detection volume. The AO correction with SIM was very successful in a thick sample for which AO correction with TFM failed.

Original languageEnglish
Pages (from-to)29021-29033
Number of pages13
JournalOptics Express
Volume29
Issue number18
DOIs
Publication statusPublished - 2021 Aug 30

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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